Best Seller

Recombinant Human BDNF Protein, CF

R&D Systems | Catalog # 11166-BD

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems CHO-derived Recombinant Human BDNF Protein (11166-BD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P23560.1

Applications

Bioactivity
View all BDNF Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human BDNF protein
His129-Arg247

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His129

Predicted Molecular Mass

13.5 kDa

SDS-PAGE

11-15 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.200-2.00 ng/mL.

Scientific Data Images for Recombinant Human BDNF Protein, CF

Recombinant Human BDNF Protein Bioactivity.

Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.200-2.00 ng/mL.

Recombinant Human BDNF Protein SDS-PAGE.

2 μg/lane of Recombinant Human BDNF Protein (Catalog # 11166-BD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 11-15 kDa.

Formulation, Preparation, and Storage

11166-BD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100-500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: BDNF

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including BDNF, nerve growth factor (NGF), neurotrophin-3 (NT3), and NT4/5. Human BDNF is initially expressed as a proprotein, which is then cleaved to yield a mature protein. Mature BDNF is a non-covalently linked homodimer, with each monomer containing antiparallel beta -strands and a characteristic cystine knot motif. Within the mature domain, human BDNF shares the identical amino acid sequence with mature mouse and rat BDNF. BDNF is strongly expressed in various regions of the brain, including the hippocampus and cerebellum, and weaker expression has been detected in the thymus, liver, spleen, heart, and lung. BDNF participates in axonal growth and pathfinding and in the modulation of dendritic growth and morphology and in later stages of development regulates synaptic transmission and plasticity and acts as a central modulator of pain. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor. BDNF signaling via TrkB is essential for adult synaptic plasticity and the formation of memories. The BDNF signaling pathway utilizes both AKT and ERK pathways to exert its pleiotrophic effects in the central nervous system. Decreased expression of BDNF is seen in many neurological diseases such as Alzheimer's disease, Parkinson's disease, Huntington's disease, and autism. Further, BDNF is proposed as a biomarker for psychiatric disorders such as bipolar disease and depression and has been implicated in posttraumatic stress disorder, phobia, and panic disorder. A single amino acid substitution, Val66Met, has been shown to lead to reduced, activity-dependent BDNF secretion and memory impairment.

References

  1. Numakawa, T. et al. (2010) Histol. Histopathol. 25:237.
  2. Egan, M.F. et al. (2003) Cell 112:257.
  3. Pruunsild, P. et al. (2007) Genomics 90:397.
  4. Bathina, S. and Das, U.N. (2015) AMS 11:1164.
  5. Binder, D.K. and Scharfman, H.E.(2004) Growth Factors 22:123.
  6. Cattaneo, A. et al. (2016) Transl. Psychiatry 6:e958.
  7. Andero, R. et al. (2011) Am J. Psychiatry 168:163.

Long Name

Brain-derived Neurotrophic Factor

Alternate Names

Abrineurin, ANON2, BULN2, Neurotrophin

Entrez Gene IDs

627 (Human); 12064 (Mouse); 24225 (Rat)

Gene Symbol

BDNF

UniProt

P23560.1

Additional BDNF Products

Product Documents for Recombinant Human BDNF Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human BDNF Protein, CF

For research use only

Citations for Recombinant Human BDNF Protein, CF

Customer Reviews for Recombinant Human BDNF Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human BDNF Protein, CF and earn rewards!

Have you used Recombinant Human BDNF Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes