Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
2406-BR-100
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit rhBMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.03-0.12 μg/mL in the presence of 15 ng/mL of Recombinant Human BMP‑4 (Catalog # 314-BP).
Source
Mouse myeloma cell line, NS0-derived human BMPR-IA/ALK-3 protein
Human BMPR-IA
(Met1-Arg152)
Accession # NP_004320
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Gln24 predicted: No results obtained, sequencing might be blocked
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40.8 kDa (monomer)
SDS-PAGE
59 kDa, reducing conditions

Product Datasheets

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2406-BR

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2406-BR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: BMPR-IA/ALK-3

Bone Morphogenetic Protein Receptor IA (BMPR‑IA), also known as ALK‑3, BRK‑1, and CD292, is a glycosylated 60‑65 kDa type I receptor in the TGF‑ beta serine/threonine kinase receptor family (1‑3). Binding of TGF‑ beta  superfamily ligands induces formation of a heterotetrameric complex that contains two chains each of a type I and a type II receptor in multiple combinations. The type II receptors phosphorylate the type I receptors which then phosphorylate and activate Smad signal transduction proteins (1, 2). Mature human BMPR‑IA consisits of a 129 amino acid (aa) extracellular domain (ECD), a 24 aa transmembrane segment, and a 356 aa cytoplasmic region that contains the tyrosine kinase domain (4). Within the ECD, human BMPR-IA shares 98% and 97% aa sequence identity with mouse and rat BMPR‑IA, respectively. BMPR‑IA is involved in the development and function of a wide range of tissues. During early embryogenesis it is required for migration of the anterior visceral endoderm (AVE) and proper development of the anterior-posterior axis (5). Tissue‑specific conditional knockout experiments have demonstrated the importance of BMPR‑IA in the development and morphogenesis of the heart, lung, palate, teeth, and mandible (6‑8). In the adult, BMPR‑IA plays a role in glucose‑stimulated insulin secretion by pancreatic beta cells, osteoclast activity and bone remodeling, reactive astrocyte‑mediated scar formation following spinal cord injury, and ovulation and fertility (9‑12).

References
  1. Wu, M.Y. and C.S. Hill (2009) Dev. Cell 16:329.
  2. Nickel, J. et al. (2009) Cytokine Growth Factor Rev. 20:367.
  3. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  4. ten Dijke, P. et al. (1993) Oncogene 8:2879.
  5. Miura, S. et al. (2010) Dev. Biol. 341:246.
  6. Gaussin, V. et al. (2002) Proc. Natl. Acad. Sci. 99:2878.
  7. Sun, J. et al. (2008) Am. J. Pathol. 172:571.
  8. Li, L. et al. (2011) Dev. Biol. 349:451.
  9. Goulley, J. et al. (2007) Cell Metab. 5:207.
  10. Kamiya, N. et al. (2008) J. Bone Miner. Res. 23:2007.
  11. Sahni, V. et al. (2010) J. Neurosci. 30:1839.
  12. Edson, M.A. et al. (2010) Mol. Endocrinol. 24:1251.
Long Name
Bone Morphogenetic Protein Receptor IA/Activin Receptor-like Kinase 3
Entrez Gene IDs
657 (Human); 12166 (Mouse)
Alternate Names
ACVRLK310q23del; ALK-3; ALK3EC 2.7.11.30; BMP type-1A receptor; BMPR1A; BMPR-1A; BMPRIA; BMPR-IA; bone morphogenetic protein receptor type-1A; bone morphogenetic protein receptor, type IA; CD292 antigen; CD292; EC 2.7.11; Serine/threonine-protein kinase receptor R5; SKR5; type II-like kinase 3

Citations for Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. BMPR1A is necessary for chondrogenesis and osteogenesis while BMPR1B prevents hypertrophic differentiation
    Authors: T Mang, K Kleinschmi, F Ploeger, A Schoeneman, S Lindemann, A Gigout
    J. Cell. Sci., 2020;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  2. BMPR1A is necessary for chondrogenesis and osteogenesis while BMPR1B prevents hypertrophic differentiation
    Authors: T Mang, K Kleinschmi, F Ploeger, A Schoeneman, S Lindemann, A Gigout
    J. Cell. Sci., 2020;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Surface Plasmon Resonance

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