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Recombinant Human BMP-4 Protein

R&D Systems | Catalog # 314-BP

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human BMP-4 Protein (314-BP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q53XC5

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all BMP-4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human BMP-4 protein
Ser293-Arg408

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser293

Predicted Molecular Mass

13 kDa (monomer)

SDS-PAGE

19-24 kDa, reducing conditions
35-41 kDa, non-reducing conditions

Activity

Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.70-7.00 ng/mL.

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272.
The ED50 for this effect is 3.00-30.0 ng/mL.

Reviewed Applications

Read 14 reviews rated 4.9 using 314-BP in the following applications:

Scientific Data Images for Recombinant Human BMP-4 Protein

Recombinant Human BMP-4 Protein Bioactivity

Recombinant Human BMP‑4 (Catalog # 314-BP) induces BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.70-7.00 ng/mL.
Recombinant Human BMP-4 Protein SDS-PAGE

Recombinant Human BMP-4 Protein SDS-PAGE

1 μg/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 19-24 kDa and 35-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 314-BP
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 50-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Carrier Free: 314-BP/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 50-200 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-4

BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1-5). The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide-linked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7
disulfide-linked heterodimer (7-9). Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13-15).

 

References

  1. Zhang, P. et al. (2008) Blood 111:1933.
  2. Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al. (2004) Stem Cells 22:457.
  5. Frank, D.B. et al. (2005) Circ. Res. 97:496.
  6. Wozney, J. et al. (1988) Science 242:1528.
  7. Cui, Y. et al. (1998) EMBO J. 17:4735.
  8. Cui, Y. et al. (2001) Genes Dev. 15:2797.
  9. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  10. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  11. Chen, D. et al. (2004) Growth Factors 22:233.
  12. Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
  13. Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
  14. Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
  15. Takada, T. et al. (2003) J. Biol. Chem. 278:43229.

Long Name

Bone Morphogenetic Protein 4

Alternate Names

BMP2B, BMP2B1, BMP4, MCOPS6, OFC11

Entrez Gene IDs

652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)

Gene Symbol

BMP4

UniProt

Q53XC5

Additional BMP-4 Products

Product Documents for Recombinant Human BMP-4 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 314-BP (with carrier)
Download SDS 314-BP/CF (carrier free)

Product Specific Notices for Recombinant Human BMP-4 Protein

For research use only

Citations for Recombinant Human BMP-4 Protein

Customer Reviews for Recombinant Human BMP-4 Protein (14)

4.9 out of 5
14 Customer Ratings
5 Stars
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4 Stars
14%
3 Stars
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  • Recombinant Human BMP-4 Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 11/05/2025
    Recombinant Human BMP-4 Protein 314-BP
  • Recombinant Human BMP-4 Protein
    Name: Anonymous
    Application: In vivo study
    Verified Customer | Posted 05/20/2023
    Recombinant Human BMP-4 Protein 314-BP
  • Recombinant Human BMP-4 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 08/23/2022
    PGCLC induction
    Recombinant Human BMP-4 Protein 314-BP
  • Recombinant Human BMP-4 Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 06/18/2022
    Recombinant Human BMP-4 Protein 314-BP
  • Recombinant Human BMP-4 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 05/01/2022
    It was used for cerebral organoid culture.
    Recombinant Human BMP-4 Protein 314-BP
  • Recombinant Human BMP-4 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 08/23/2021
    Recombinant Human BMP-4 Protein 314-BP
  • Recombinant Human BMP-4 Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 10/13/2020
    Recombinant Human BMP-4 Protein 314-BP
  • Recombinant Human BMP-4 Protein
    Name: CHING-WEN HSIAO
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 10/13/2020
  • Recombinant Human BMP-4 Protein
    Name: Lisa McDonnell
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 07/24/2020
  • Recombinant Human BMP-4 Protein
    Name: Pablo Hurtado
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 05/28/2020
  • Recombinant Human BMP-4 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 04/07/2020
    Recombinant Human BMP-4 Protein 314-BP
  • Recombinant Human BMP-4 Protein
    Name: Danny Tam
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 12/21/2017
  • Recombinant Human BMP-4 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 05/19/2016
  • Recombinant Human BMP-4 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 05/18/2016
    Recombinant Human BMP-4 Protein 314-BP

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FAQs for Recombinant Human BMP-4 Protein

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  • Q: Can Recombinant Human BMP-4 Protein (Catalog # 314-BP or 314-BP/CF) be reconstituted in PBS or water?

    A: Recombinant Human BMP-4 (Catalog # 314-BP or 314-BP/CF) needs to be reconstituted to 50-200 μg/mL in sterile 4 mM HCl containing 0.1% BSA, or 4 mM HCl to ensure optimal recovery, stability, and activity. This protein is hydrophobic by nature, so it requires an acidic buffer for reconstitution to go completely into solution. Stability testing has been evaluated on the protein with these reconstitution conditions; we can guarantee performance under these reconstitution conditions. When the protein is diluted with the cell culture media or buffers to the working concentration, the concentration of the acid should be buffered or negligible.

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