Recombinant Human BTN2A1/Butyrophilin 2A1 Protein, CF

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Recombinant Human BTN2A1/Butyrophilin 2A1 Protein Bioactivity
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Recombinant Human BTN2A1/Butyrophilin 2A1 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human BTN2A1 is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human DC‑SIGN/CD209 Fc Chimera (Catalog # 161-DC) that produces 50% of the optimal binding response is approximately
0.1-0.5 μg/mL.
Human embryonic kidney cell, HEK293-derived human BTN2A1 protein
Gln29-Ala248, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
No results obtained. Gln29 predicted
Predicted Molecular Mass
25 kDa
39-44 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human BTN2A1/Butyrophilin 2A1 Protein Bioactivity View Larger

When Recombinant Human BTN2A1/Butyrophilin 2A1 (Catalog # 9058-BT) is coated at 1 µg/mL, Recombinant Human DC-SIGN/CD209 Fc Chimera (Catalog # 161-DC) binds with a ED50 of 0.1-0.5 μg/mL.

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Background: BTN2A1

Butyrophilin 2A1 (BTN2A1) is an approximately 70 kDa widely expressed and variably glycosylated type I transmembrane glycoprotein (1). Mature human Butyrophilin 2A1 consisits of a 220 amino acid (aa) extracellular domain with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 258 aa cytoplasmic domain (2). Alternative splicing generates additional isoforms of human Butyrophilin 2A1 that lack the first Ig-like domain or transmembrane segment as well as isoforms with substitutions and deletions in the cytoplasmic region. BTN2A1 is widely expressed including on colonic epithelial cells, on immune cells, and in milk fat globules (3, 4). It binds to the C-type lectin DC-SIGN on monocyte-derived dendritic cells, and this interaction can be blocked by soluble gp130 from HIV (3). The polymorphism of BTN2A1 has been associated with metabolic syndrome, type II diabetes mellitus, chronic kidney disease, and hypertension (5, 6).

  1. Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
  2. Tazi-Ahnini, R. et al. (1997) Immunogenetics 47:55.
  3. Malcherek, G. et al. (2007) J. Immunol. 179:3804.
  4. Cavaletto, M. et al. (2002) Proteomics 2:850.
  5. Oguri, M. et al.(2011) J. Med. Genet. 48:787.
  6. Horibe, H. et al. (2014) Mol. Med. Rep. 9:808.
Long Name
Butyrophilin Subfamily 2 Member A1
Entrez Gene IDs
11120 (Human)
Alternate Names
BK14H9.1; BT2.1; BTF1; BTF1BT2.1butyrophilin BTF1; BTN2A1; butyrophilin subfamily 2 member A1; butyrophilin, subfamily 2, member A1; DJ3E1.1; FLJ36567

Citation for Recombinant Human BTN2A1/Butyrophilin 2A1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. BTN2A1, an immune checkpoint targeting Vgamma9Vdelta2 T cell cytotoxicity against malignant cells
    Authors: CE Cano, C Pasero, A De Gassart, C Kerneur, M Gabriac, M Fullana, E Granarolo, R Hoet, E Scotet, C Rafia, T Hermman, C Imbert, L Gorvel, N Vey, A Briantais, AC le Floch, D Olive
    Cell Reports, 2021;36(2):109359.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Capture


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