Recombinant Human BTN2A1/Butyrophilin 2A1 Protein, CF

    
Datasheet / CoA / SDS
Product Details
FAQs
Supplemental Products
Reviews
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human BTN2A1 is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human DC‑SIGN/CD209 Fc Chimera (Catalog # 161-DC) that produces 50% of the optimal binding response is approximately
    0.1-0.5 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived human BTN2A1 protein
    Gln29-Ala248, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained. Gln29 predicted
  • Predicted Molecular Mass
    25 kDa
  • SDS-PAGE
    39-44 kDa, reducing conditions
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

9058-BT
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 100 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
When Recombinant Human BTN2A1/Butyrophilin 2A1 (Catalog # 9058-BT) is coated at 1 µg/mL, Recombinant Human DC-SIGN/CD209 Fc Chimera (Catalog # 161-DC) binds with a ED50 of
0.1-0.5 μg/mL.
Background: BTN2A1
Butyrophilin 2A1 (BTN2A1) is an approximately 70 kDa widely expressed and variably glycosylated type I transmembrane glycoprotein (1). Mature human Butyrophilin 2A1 consisits of a 220 amino acid (aa) extracellular domain with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 258 aa cytoplasmic domain (2). Alternative splicing generates additional isoforms of human Butyrophilin 2A1 that lack the first Ig-like domain or transmembrane segment as well as isoforms with substitutions and deletions in the cytoplasmic region. BTN2A1 is widely expressed including on colonic epithelial cells, on immune cells, and in milk fat globules (3, 4). It binds to the C-type lectin DC-SIGN on monocyte-derived dendritic cells, and this interaction can be blocked by soluble gp130 from HIV (3). The polymorphism of BTN2A1 has been associated with metabolic syndrome, type II diabetes mellitus, chronic kidney disease, and hypertension (5, 6).
  • References:
    1. Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
    2. Tazi-Ahnini, R. et al. (1997) Immunogenetics 47:55.
    3. Malcherek, G. et al. (2007) J. Immunol. 179:3804.
    4. Cavaletto, M. et al. (2002) Proteomics 2:850.
    5. Oguri, M. et al.(2011) J. Med. Genet. 48:787.
    6. Horibe, H. et al. (2014) Mol. Med. Rep. 9:808.
  • Long Name:
    Butyrophilin Subfamily 2 Member A1
  • Entrez Gene IDs:
    11120 (Human)
  • Alternate Names:
    BK14H9.1; BT2.1; BTF1; BTF1BT2.1butyrophilin BTF1; BTN2A1; butyrophilin subfamily 2 member A1; butyrophilin, subfamily 2, member A1; DJ3E1.1; FLJ36567
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 31  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag APC-conjugated Antibody

ICFlow IC050A  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PE-conjugated Antibody

ICFlow IC050P 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Antibody

WB , Flow , AP MAB050R 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Biotinylated Antibody

WB BAM050 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PerCP-conjugated Antibody

ICFlow IC050C  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Human BTN2A1/Butyrophilin 2A1 Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Related Products
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee