Recombinant Human BTN2A1/Butyrophilin 2A1 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human BTN2A1 is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human DC‑SIGN/CD209 Fc Chimera (Catalog # 161-DC) that produces 50% of the optimal binding response is approximately
    0.1-0.5 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Gln29-Ala248, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained. Gln29 predicted
  • Predicted Molecular Mass
    25 kDa
  • SDS-PAGE
    39-44 kDa, reducing conditions
9058-BT
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Human BTN2A1/Butyrophilin 2A1 (Catalog # 9058-BT) is coated at 1 µg/mL, Recombinant Human DC-SIGN/CD209 Fc Chimera (Catalog # 161-DC) binds with a ED50 of
0.1-0.5 μg/mL.
Background: BTN2A1
Butyrophilin 2A1 (BTN2A1) is an approximately 70 kDa widely expressed and variably glycosylated type I transmembrane glycoprotein (1). Mature human Butyrophilin 2A1 consisits of a 220 amino acid (aa) extracellular domain with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 258 aa cytoplasmic domain (2). Alternative splicing generates additional isoforms of human Butyrophilin 2A1 that lack the first Ig-like domain or transmembrane segment as well as isoforms with substitutions and deletions in the cytoplasmic region. BTN2A1 is widely expressed including on colonic epithelial cells, on immune cells, and in milk fat globules (3, 4). It binds to the C-type lectin DC-SIGN on monocyte-derived dendritic cells, and this interaction can be blocked by soluble gp130 from HIV (3). The polymorphism of BTN2A1 has been associated with metabolic syndrome, type II diabetes mellitus, chronic kidney disease, and hypertension (5, 6).
  • References:
    1. Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
    2. Tazi-Ahnini, R. et al. (1997) Immunogenetics 47:55.
    3. Malcherek, G. et al. (2007) J. Immunol. 179:3804.
    4. Cavaletto, M. et al. (2002) Proteomics 2:850.
    5. Oguri, M. et al.(2011) J. Med. Genet. 48:787.
    6. Horibe, H. et al. (2014) Mol. Med. Rep. 9:808.
  • Long Name:
    Butyrophilin Subfamily 2 Member A1
  • Entrez Gene IDs:
    11120 (Human)
  • Alternate Names:
    BK14H9.1; BT2.1; BTF1; BTF1BT2.1butyrophilin BTF1; BTN2A1; butyrophilin subfamily 2 member A1; butyrophilin, subfamily 2, member A1; DJ3E1.1; FLJ36567
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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