Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

Newer Version Available: 9136-DC
A New and Improved rh DC-SIGN is Now Available! The new protein has enhanced purity and a HA-tag!
Catalog # Availability Size / Price Qty
161-DC-050
Product Details
Citations (14)
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Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. When 5 x 104 cells/well are added to Recombinant Human DC‑SIGN/CD209 Fc Chimera coated plates (5 µg/mL with 100 µL/well), approximately 50-80% of added cells will adhere after 1 hour at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human DC-SIGN/CD209 protein
MD Human IgG1
(Pro100-Lys330)
IEGR Human DC-SIGN
(Lys62-Ala404)
Accession # Q9NNX6
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
65.7 kDa (monomer)
SDS-PAGE
65-75 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

161-DC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: DC-SIGN/CD209

Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca2+-dependent carbohydrate-binding lectin domain (2). Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms (3). DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (5, 6).

References
  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
  4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
  5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
  6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.
Long Name
Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1
Entrez Gene IDs
30835 (Human); 170786 (Mouse); 102121984 (Cynomolgus Monkey)
Alternate Names
CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Citations for Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Schistosoma mansoni soluble egg antigen (SEA) and recombinant Omega-1 modulate induced CD4+ T-lymphocyte responses and HIV-1 infection in vitro
    Authors: EE Mouser, G Pollakis, HH Smits, J Thomas, M Yazdanbakh, EC de Jong, WA Paxton
    PLoS Pathog., 2019;15(9):e1007924.
    Species: Parasite - Schistosoma mansoni
    Sample Types: Protein
    Applications: Bioassay
  2. A recombinant human IgG1 Fc multimer designed to mimic the active fraction of IVIG in autoimmunity
    Authors: X Zhang, J Owens, HS Olsen, E So, E Burch, MC McCroskey, X Li, GL Weber, D Bennett, D Rybin, H Zhou, H Hao, EY Mérigeon, DS Block, G LaRosa, SE Strome
    JCI Insight, 2019;4(2):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  3. An innovative immunotherapeutic strategy for ovarian cancer: CLEC10A and glycomimetic peptides
    Authors: LL Eggink, KF Roby, R Cote, J Kenneth Ho
    J Immunother Cancer, 2018;6(1):28.
    Species: Human
    Sample Types: Peptide
    Applications: Binding Assay
  4. Overexpression of Ebola virus envelope GP1 protein
    Authors: Z Zou, J Misasi, N Sullivan, PD Sun
    Protein Expr. Purif., 2017;0(0):.
    Species: Virus - Ebola
    Sample Types: Protein
    Applications: Enzyme Assay
  5. Brugia malayi Antigen (BmA) Inhibits HIV-1 Trans-Infection but Neither BmA nor ES-62 Alter HIV-1 Infectivity of DC Induced CD4+ Th-Cells.
    Authors: Mouser E, Pollakis G, Yazdanbakhsh M, Harnett W, de Jong E, Paxton W
    PLoS ONE, 2016;11(1):e0146527.
    Species: Nematode - Brugia malayi
    Sample Types: Protein
    Applications: ELISA Developmet
  6. Allergy-Protective Arabinogalactan Modulates Human Dendritic Cells via C-Type Lectins and Inhibition of NF-kappaB.
    Authors: Peters M, Guidato P, Peters K, Megger D, Sitek B, Classen B, Heise E, Bufe A
    J Immunol, 2015;196(4):1626-35.
  7. Colorectal mucus binds DC-SIGN and inhibits HIV-1 trans-infection of CD4+ T-lymphocytes.
    Authors: Stax M, Mouser E, van Montfort T, Sanders R, de Vries H, Dekker H, Herrera C, Speijer D, Pollakis G, Paxton W
    PLoS ONE, 2015;10(3):e0122020.
    Species: Human
    Sample Types: Mucus
    Applications: Bioassay
  8. The clinical significance of DC-SIGN and DC-SIGNR, which are novel markers expressed in human colon cancer.
    Authors: Jiang, Yanmei, Zhang, Changfu, Chen, Kai, Chen, Zhe, Sun, Zhigang, Zhang, Zhuqing, Ding, Dongbing, Ren, Shuangyi, Zuo, Yunfei
    PLoS ONE, 2014;9(12):e114748.
    Species: Human
    Sample Types: Serum
    Applications: ELISA (Standard)
  9. Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite.
    Authors: Emara M, Royer PJ, Mahdavi J
    J. Biol. Chem., 2012;287(8):5756-63.
    Species: Canine
    Sample Types: Peptide
    Applications: ELISA (Detection)
  10. Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2.
    Authors: Johnson TR, McLellan JS, Graham BS
    J. Virol., 2012;86(3):1339-47.
    Species: Virus
    Sample Types: Virus
    Applications: Surface Plasmon Resonance
  11. Pichia pastoris-produced mucin-type fusion proteins with multivalent O-glycan substitution as targeting molecules for mannose-specific receptors of the immune system.
    Authors: Gustafsson A, Sjoblom M, Strindelius L, Johansson T, Fleckenstein T, Chatzissavidou N, Lindberg L, Angstrom J, Rova U, Holgersson J
    Glycobiology, 2011;21(8):1071-86.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  12. Semen clusterin is a novel DC-SIGN ligand.
    Authors: Sabatte J, Faigle W, Ceballos A, Morelle W, Rodrígues CR, Lenicov FR, Thepaut M, Fieschi F, Malchiodi E, Fernandez M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S
    J. Immunol., 2011;187(10):5299-309.
    Species: Human
    Sample Types: Seminal Plasma
    Applications: Surface Plasmon Resonance
  13. Mucin 6 in seminal plasma binds DC-SIGN and potently blocks dendritic cell mediated transfer of HIV-1 to CD4(+) T-lymphocytes.
    Authors: Stax MJ, van Montfort T, Sprenger RR, Melchers M, Sanders RW, van Leeuwen E, Repping S, Pollakis G, Speijer D, Paxton WA
    Virology, 2009;391(2):203-11.
    Species: Human
    Sample Types: Seminal Plasma
    Applications: Western Blot (Detection)
  14. Glycosylation-dependent interactions of C-type lectin DC-SIGN with colorectal tumor-associated Lewis glycans impair the function and differentiation of monocyte-derived dendritic cells.
    Authors: Nonaka M, Ma BY, Murai R, Nakamura N, Baba M, Kawasaki N, Hodohara K, Asano S, Kawasaki T
    J. Immunol., 2008;180(5):3347-56.

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