Cathepsin E is an intracellular aspartic protease of the pepsin family (1). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsin D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (2). Both enzymes are efficient in cleaving Swedish mutant of amyloid precursor protein (APP) at the beta site but show almost no reactivity with wild-type APP (3). Human Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑17), a propeptide (residues 18‑53), and a mature chain (residues 54‑396) (4).
Recombinant Human Cathepsin E Protein, CF
R&D Systems | Catalog # 1294-AS
Key Product Details
- R&D Systems NS0-derived Recombinant Human Cathepsin E Protein (1294-AS)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Structure / Form
Applications
Product Specifications
Source
Gln18-Pro396, with a C-terminal 10-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001).
The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
1294-AS
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM MES, 150 mM NaCl, pH 6.5. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: Cathepsin E
References
- Kay, J. and P.J. Tatnell (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. eds.), p. 33, Academic Press, San Diego.
- Tsukuba, T. et al. (2000) Mol. Cells 10:601.
- Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.
- Azuma, T. et al. (1989) J. Biol. Chem. 264:16748.
Alternate Names
Gene Symbol
UniProt
Additional Cathepsin E Products
Product Documents for Recombinant Human Cathepsin E Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Cathepsin E Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human Cathepsin E Protein, CF
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Protocols
View specific protocols for Recombinant Human Cathepsin E Protein, CF (1294-AS):
- Assay Buffer: 0.1 M NaOAc, 0.5 M NaCl, pH 3.5
- Recombinant Human Cathepsin E (rhCathepsin E) (Catalog # 1294-AS)
- Fluorogenic Peptide Substrate I: MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 (Catalog # ES001)
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rhCathepsin E to 1.0 µg/mL in Assay Buffer.
- Incubate at room temperature for 30 minutes (required to fully activate).
- Dilute activated rhCathepsin E to 0.2 ng/µL in Assay Buffer.
- Dilute Substrate to 40 µM in Assay Buffer.
- Load 50 µL of the 0.2 ng/µL rhCathepsin E into a black well plate, and start the reaction by adding 50 µL of 40 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 40 µM Substrate without any rhCathepsin E.
- Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).
Per Well:
- rhCathepsin E: 0.01 µg
- Substrate: 20 µM