Mca-PLGL-Dpa-AR-NH2 Fluorogenic MMP Substrate

Substrate for MMP-1, MMP-2, MMP-7, MMP-8, MMP-9, MMP-12, MMP-13, MMP-14, MMP-15, MMP-16, Cathepsin D, and Cathepsin E
Catalog # Availability Size / Price Qty
ES001
Product Details
Citations (23)
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Mca-PLGL-Dpa-AR-NH2 Fluorogenic MMP Substrate Summary

Activity
The peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2, 4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence. It is also an excellent substrate for active Cathepsins D and E.
Source

Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2
Mca: (7-Methoxycoumarin-4-yl)acetyl, Dpa: N-3-(2, 4-Dinitrophenyl)-L-2,3-diaminopropionyl.
Predicted Molecular Mass
1093 Da

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

ES001

Formulation Supplied as a stock solution in dimethyl sulfoxide (DMSO) at a concentration of 7.99 mg/mL or 6.07 mM.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light.
Reconstitution Calculator

Reconstitution Calculator

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Citations for Mca-PLGL-Dpa-AR-NH2 Fluorogenic MMP Substrate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Design and synthesis of selective and blood-brain barrier-permeable hydroxamate-based gelatinase inhibitors
    Authors: A Bertran, D Khomiak, A Konopka, E Rejmak, E Bulska, J Seco, L Kaczmarek, T Tarragó, R Prades
    Bioorg. Chem., 2019;0(0):103365.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  2. Matrix metalloproteinases inactivate the proinflammatory functions of secreted moonlighting tryptophanyl-tRNA synthetase
    Authors: PG Jobin, N Solis, Y Machado, PA Bell, NH Kwon, S Kim, CM Overall, GS Butler
    J. Biol. Chem., 2019;0(0):.
    Applications: Enzyme Assay Substrate
  3. Mitochondrial function in liver cells is resistant to perturbations in NAD+ salvage capacity
    Authors: M Dall, SAJ Trammell, M Asping, AS Hassing, M Agerholm, SG Vienberg, MP Gillum, S Larsen, JT Treebak
    J. Biol. Chem., 2019;0(0):.
    Species: Human
    Sample Types:
    Applications: Enzyme Assay
  4. MMP-12, Secreted by Pro-Inflammatory Macrophages, Targets Endoglin in Human Macrophages and Endothelial Cells
    Authors: M Aristorena, E Gallardo-V, M Vicen, M de Las Cas, L Ojeda-Fern, C Nieto, FJ Blanco, AC Valbuena-D, LM Botella, P Nachtigal, AL Corbi, M Colmenares, C Bernabeu
    Int J Mol Sci, 2019;20(12):.
    Species: Human
    Sample Types: Protein
  5. Evaluation of an Allosteric BACE inhibitor peptide to identify mimetics that can interact with the Loop F Region of the enzyme and prevent APP cleavage
    Authors: J Campagna, K Vadivel, B Jagodzinsk, M Jun, T Bilousova, P Spilman, V John
    J. Mol. Biol., 2018;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. Matrix Metalloproteinase-Mediated Blood-Brain Barrier Dysfunction in Epilepsy
    Authors: RG Rempe, AMS Hartz, ELB Soldner, BS Sokola, SR Alluri, EL Abner, RJ Kryscio, A Pekcec, J Schlichtig, B Bauer
    J. Neurosci., 2018;0(0):.
    Species: Rat
    Sample Types: Protein
    Applications: Bioassay
  7. Novel Arginine-containing Macrocyclic MMP Inhibitors: Synthesis, 99mTc-labeling, and Evaluation
    Authors: Y Ye, J Toczek, K Gona, HY Kim, J Han, M Razavian, R Golestani, J Zhang, TL Wu, M Ghosh, JJ Jung, MM Sadeghi
    Sci Rep, 2018;8(1):11647.
    Species: Human
    Sample Types: Recombinant Protein
  8. The selectivity of galardin and an azasugar-based hydroxamate compound for human matrix metalloproteases and bacterial metalloproteases
    Authors: I Sylte, R Dawadi, N Malla, S von Hofste, TM Nguyen, AI Solli, E Berg, OA Adekoya, G Svineng, JO Winberg
    PLoS ONE, 2018;13(8):e0200237.
    Species: Human
    Sample Types: Protein
  9. Acute- and late-phase matrix metalloproteinase (MMP)-9 activity is comparable in female and male rats after peripheral nerve injury
    Authors: AG Remacle, SK Hullugundi, J Dolkas, M Angert, AV Chernov, AY Strongin, VI Shubayev
    J Neuroinflammation, 2018;15(1):89.
    Species: Rat
    Sample Types: Urine
    Applications: Bioassay
  10. Coagulating Colubrids: Evolutionary, Pathophysiological and Biodiscovery Implications of Venom Variations between Boomslang (Dispholidus typus) and Twig Snake (Thelotornis mossambicanus)
    Authors: J Debono, J Dobson, NR Casewell, A Romilio, B Li, N Kurniawan, K Mardon, V Weisbecker, A Nouwens, HF Kwok, BG Fry
    Toxins (Basel), 2017;9(5):.
    Species: Snake
    Sample Types: Venom
    Applications: Enzyme Assay Substrate
  11. Harnessing the natural inhibitory domain to control TNF? Converting Enzyme (TACE) activity in vivo
    Sci Rep, 2016;6(0):35598.
  12. Insulin-Like Growth Factor I (IGF-I) Expressed from an AAV1 Vector Leads to a Complete Reversion of Liver Cirrhosis in Rats
    PLoS ONE, 2016;11(9):e0162955.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  13. High-grade serous ovarian cancer cell lines exhibit heterogeneous responses to growth factor stimulation.
    Authors: Bourgeois D, Kabarowski K, Porubsky V, Kreeger P
    Cancer Cell Int, 2015;15(0):112.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Plantamajoside, a potential anti-tumor herbal medicine inhibits breast cancer growth and pulmonary metastasis by decreasing the activity of matrix metalloproteinase-9 and -2.
    Authors: Pei S, Yang X, Wang H, Zhang H, Zhou B, Zhang D, Lin D
    BMC Cancer, 2015;15(0):965.
    Species: Human
    Sample Types: Recombinant Protein
  15. Soft matrix is a natural stimulator for cellular invasiveness.
    Authors: Gu, Zhizhan, Liu, Fei, Tonkova, Elina A, Lee, Soo Youn, Tschumperlin, Daniel J, Brenner, Michael
    Mol Biol Cell, 2014;25(4):457-69.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  16. Cellular contractility and extracellular matrix stiffness regulate matrix metalloproteinase activity in pancreatic cancer cells.
    Authors: Haage A, Schneider I
    FASEB J, 2014;28(8):3589-99.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Novel proteolytic microvesicles released from human macrophages after exposure to tobacco smoke.
    Authors: Li C, Liu Y, Chen Y, Yu D, Williams K, Liu M
    Am J Pathol, 2013;182(5):1552-62.
    Species: Human
    Sample Types: Cell Lysates
  18. Simple pseudo-dipeptides with a P2' glutamate: a novel inhibitor family of matrix metalloproteases and other metzincins.
    Authors: Devel L, Beau F, Amoura M, Vera L, Cassar-Lajeunesse E, Garcia S, Czarny B, Stura E, Dive V
    J Biol Chem, 2012;287(32):26647-56.
    Species: N/A
    Sample Types: N/A
    Applications: Enzyme Assay Substrate
  19. The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea.
    Authors: Zeeuwen PL, van Vlijmen-Willems IM, Cheng T
    FASEB J., 2010;24(10):3744-55.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay Substrate
  20. Decreased pathology and prolonged survival of human DC-SIGN transgenic mice during mycobacterial infection.
    Authors: Schaefer M, Reiling N, Fessler C, Stephani J, Taniuchi I, Hatam F, Yildirim AO, Fehrenbach H, Walter K, Ruland J, Wagner H, Ehlers S, Sparwasser T
    J. Immunol., 2008;180(10):6836-45.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. IFN-gamma regulation of vacuolar pH, cathepsin D processing and autophagy in mammary epithelial cells.
    Authors: Khalkhali-Ellis Z, Abbott DE, Bailey CM, Goossens W, Margaryan NV, Gluck SL, Reuveni M, Hendrix MJ
    J. Cell. Biochem., 2008;105(1):208-18.
    Species: Human
    Sample Types: Cell Lysates
  22. Microparticles of human atherosclerotic plaques enhance the shedding of the tumor necrosis factor-alpha converting enzyme/ADAM17 substrates, tumor necrosis factor and tumor necrosis factor receptor-1.
    Authors: Canault M, Leroyer AS, Peiretti F, Leseche G, Tedgui A, Bonardo B, Alessi MC, Boulanger CM, Nalbone G
    Am. J. Pathol., 2007;171(5):1713-23.
    Species: Human
    Sample Types: Biofilm
    Applications: Bioassay
  23. Targeting ADAM-mediated ligand cleavage to inhibit HER3 and EGFR pathways in non-small cell lung cancer.
    Authors: Zhou BB, Peyton M, He B, Liu C, Girard L, Caudler E, Lo Y, Baribaud F, Mikami I, Reguart N, Yang G, Li Y, Yao W, Vaddi K, Gazdar AF, Friedman SM, Jablons DM, Newton RC, Fridman JS, Minna JD, Scherle PA
    Cancer Cell, 2006;10(1):39-50.
    Species: Human
    Sample Types: Protein

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