Recombinant Human CCL11/Eotaxin Protein

Carrier Free

Catalog # Availability Size / Price Qty
320-EO-020/CF
320-EO-100/CF

With Carrier

Catalog # Availability Size / Price Qty
320-EO-020
320-EO-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (29)
FAQs
Reviews (1)

Recombinant Human CCL11/Eotaxin Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR3. The ED50 for this effect is 1-5 ng/mL.
Source
E. coli-derived human CCL11/Eotaxin protein
Gly24-Pro97
Accession #
N-terminal Sequence
Analysis
Gly24
Predicted Molecular Mass
8.4 kDa

Product Datasheets

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320-EO (with carrier)

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320-EO/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

320-EO

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

320-EO/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL11/Eotaxin

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2 and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1 ‑ 3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4).

References
  1. Kitamura, M. et al. (1996) J. Biol. Chem 271:7725.
  2. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
  3. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
  4. Choe, H. et al. (1996) Cell 85:1135.
 
Entrez Gene IDs
6356 (Human); 20292 (Mouse)
Alternate Names
C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Citations for Recombinant Human CCL11/Eotaxin Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Chemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors
    Authors: L Déry, G Charest, B Guérin, M Akbari, D Fortin
    International Journal of Molecular Sciences, 2021;22(22):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The Release Kinetics of Eosinophil Peroxidase and Mitochondrial DNA Is Different in Association with Eosinophil Extracellular Trap Formation
    Authors: N Germic, T Fettrelet, D Stojkov, A Hosseini, MP Horn, A Karaulov, D Simon, S Yousefi, HU Simon
    Cells, 2021;10(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. A field-applicable method for flow cytometric analysis of granulocyte activation: Cryopreservation of fixed granulocytes
    Authors: K de Ruiter, S van Staver, B Hilvering, E Knol, N Vrisekoop, L Koenderman, M Yazdanbakh
    Cytometry A, 2018;0(0):.
    Species: Human
    Sample Types: Whole Blood
    Applications: Bioassay
  4. Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study
    Authors: Benjamin Malard
    Artif Organs, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: Hemofiltration Procedure
  5. The soluble guanylyl cyclase activator BAY 60-2770 inhibits murine allergic airways inflammation and human eosinophil chemotaxis
    Authors: Edson Antunes
    Pulm Pharmacol Ther, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Anti-Inflammatory Effects of Metformin Irrespective of Diabetes Status
    Circ Res, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: Bioassay
  7. Extracellular Microvesicle Production by Human Eosinophils Activated by Inflammatory Stimuli
    Authors: Rossana C N Melo
    Front Cell Dev Biol, 2016;4(0):117.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Helminth infections and type 2 diabetes: a cluster-randomized placebo controlled SUGARSPIN trial in Nangapanda, Flores, Indonesia.
    Authors: Tahapary D, de Ruiter K, Martin I, van Lieshout L, Guigas B, Soewondo P, Djuardi Y, Wiria A, Mayboroda O, Houwing-Duistermaat J, Tasman H, Sartono E, Yazdanbakhsh M, Smit J, Supali T
    BMC Infect Dis, 2015;15(0):133.
    Species: Human
    Sample Types: Whole Blood
    Applications: Bioassay
  9. Functional analysis of free fatty acid receptor GPR120 in human eosinophils: implications in metabolic homeostasis.
    Authors: Konno, Yasunori, Ueki, Shigehar, Takeda, Masahide, Kobayashi, Yoshiki, Tamaki, Mami, Moritoki, Yuki, Oyamada, Hajime, Itoga, Masamich, Kayaba, Hiroyuki, Omokawa, Ayumi, Hirokawa, Makoto
    PLoS ONE, 2015;10(3):e0120386.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Eotaxin-1 promotes prostate cancer cell invasion via activation of the CCR3-ERK pathway and upregulation of MMP-3 expression.
    Authors: Zhu F, Liu P, Li J, Zhang Y
    Oncol Rep, 2014;31(5):2049-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. NADPH oxidase-independent formation of extracellular DNA traps by basophils.
    Authors: Morshed M, Hlushchuk R, Simon D, Walls A, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon H, Yousefi S
    J Immunol, 2014;192(11):5314-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. The internal architecture of leukocyte lipid body organelles captured by three-dimensional electron microscopy tomography.
    Authors: Melo, Rossana, Paganoti, Guillher, Dvorak, Ann M, Weller, Peter F
    PLoS ONE, 2013;8(3):e59578.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Novel association between vasoactive intestinal peptide and CRTH2 receptor in recruiting eosinophils: a possible biochemical mechanism for allergic eosinophilic inflammation of the airways.
    Authors: El-Shazly A, Begon D, Kustermans G, Arafa M, Dortu E, Henket M, Lefebvre P, Louis R, Delvenne P
    J Biol Chem, 2013;288(2):1374-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. The ageing systemic milieu negatively regulates neurogenesis and cognitive function.
    Authors: Villeda SA, Luo J, Mosher KI, Zou B, Britschgi M, Bieri G, Stan TM, Fainberg N, Ding Z, Eggel A, Lucin KM, Czirr E, Park JS, Couillard-Despres S, Aigner L, Li G, Peskind ER, Kaye JA, Quinn JF, Galasko DR, Xie XS, Rando TA, Wyss-Coray T
    Nature, 2011;477(7362):90-4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Leptin enhances survival and induces migration, degranulation, and cytokine synthesis of human basophils.
    Authors: Suzukawa M, Nagase H, Ogahara I, Han K, Tashimo H, Shibui A, Koketsu R, Nakae S, Yamaguchi M, Ohta K
    J. Immunol., 2011;186(9):5254-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Osteopontin is expressed and functional in human eosinophils.
    Authors: Puxeddu I, Berkman N, Ribatti D
    Allergy, 2010;65(2):168-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation.
    Authors: Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M
    Blood, 2008;112(3):493-503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008;181(11):8053-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
    Authors: Osei-Kumah A, Smith R, Clifton VL
    Cytokine, 2008;43(2):187-93.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  21. Novel antiviral activity of chemokines.
    Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
    Virology, 2006;350(2):484-92.
    Species: Virus
    Sample Types: Virus
    Applications: Binding Assay
  22. Functional analysis of the chemokine receptor CCR3 on airway epithelial cells.
    Authors: Beck LA, Tancowny B, Brummet ME, Asaki SY, Curry SL, Penno MB, Foster M, Bahl A, Stellato C
    J. Immunol., 2006;177(5):3344-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
  24. CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1.
    Authors: Yamamoto H, Nagata M, Sakamoto Y
    Ann. Allergy Asthma Immunol., 2005;94(2):292-300.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  25. Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission.
    Authors: Hajnicka V, Vancova I, Kocakova P, Slovak M, Gasperik J, Slavikova M, Hails RS, Labuda M, Nuttall PA
    Parasitology, 2005;130(0):333-42.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  26. The CC chemokine eotaxin/CCL11 has a selective profibrogenic effect on human lung fibroblasts.
    Authors: Puxeddu I, Bader R, Piliponsky AM, Reich R, Levi-Schaffer F, Berkman N
    J. Allergy Clin. Immunol., 2005;117(1):103-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Identification of full, partial and inverse CC chemokine receptor 3 agonists using [35S]GTPgammaS binding.
    Authors: Wan Y, Jakway JP, Qiu H, Shah H, Garlisi CG, Tian F, Ting P, Hesk D, Egan RW, Billah MM, Umland SP
    Eur. J. Pharmacol., 2002;456(1):1-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Chemoattractant factors in breast milk from allergic and nonallergic mothers.
    Authors: Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP
    Pediatr. Res., 2000;47(5):592-7.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  29. Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells.
    Authors: Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B
    iScience, 0;24(12):103433.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human CCL11/Eotaxin Protein
By Anonymous on 11/17/2017
Application: Immunoassay Standard