Recombinant Human CCL3/MIP-1 alpha Protein, CF

(30 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to chemoattract 2-day cultured human monocytes. The ED50 for this effect is 2‑10 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 3‑10 ng/mL.
  • Source
    E. coli-derived Ala27-Ala92
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala27
  • Predicted Molecular Mass
    7.5 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
270-LD/CF
 
270-LD
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human CCL3/MIP-1 alpha Isoform LD78a (Catalog # 270-LD/CF) chemoattracts the BaF3 mouse pro-B cells transfected with human CCR5. The ED50 for this effect is 3-10 ng/mL.
1 μg/lane of Recombinant Human CCL3/MIP-1 alpha Isoform LD78a was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 12 kDa.
Background: CCL3/MIP-1 alpha
CCL3, also known as macrophage inflammatory protein 1 alpha (MIP‑1 alpha ) and LD78, is a member of the beta or CC subfamily of chemokines and is closely related to CCL4/MIP‑1 beta. Chemokines comprise a large family of small secreted proteins that are involved in immune and inflammatory responses. CCL3 expression can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells (1). Mature human CCL3 shares 70%‑74% amino acid sequence identity with mouse, rat, and cotton rat CCL3 (2). CCL3 is an approximately 8 kDa chemokine that forms complexes with sulfated proteoglycans (3, 4). In a reversible process, CCL3 associates into noncovalently‑linked dimers which then form tetramers and high molecular weight polymers (5, 6). These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine (6). CCL3 exerts its biological functions through interactions with CCR1, CCR3, and CCR5 (1). It is cleared from the extracellular space by internalization via the decoy chemokine receptor D6 (7). CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types including activated T cells, NK cells, neutrophils, monocytes, immature dendritic cells, and eosinophils (1, 8‑10). CCL3 is also known as stem cell inhibitor (SCI) and can inhibit the proliferation of hematopoietic progenitor cells (3). CCL3 bioactivity contributes to tumor metastasis and the inflammatory components of viral infection, rheumatoid arthritis, and hepatitis (11‑14), although it also can suppress the replication of HIV (15). CCL3 additionally promotes hyperalgesia by sensitizing sensory neurons to TRPV1‑mediated noxious stimulation (16).
  • References:
    1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
    2. Obaru, K. et al. (1986) J. Biochem. 99:885.
    3. Graham, G.J. et al. (1990) Nature 344:442.
    4. Wagner, L. et al. (1998) Nature 391:908.
    5. Graham, G.J. et al. (1994) J. Biol. Chem. 269:4974.
    6. Ren, M. et al. (2010) EMBO J. 29:3952.
    7. Weber, M. et al. (2004) Mol. Biol. Cell 15:2492
    8. Taub, D.D. et al. (1993) Science 260:355.
    9. Bernardini, G. et al. (2008) Blood 111:3626.
    10. Lee, S.C. et al. (2000) J. Immunol. 164:3392.
    11. Wu, Y. et al. (2008) J. Immunol. 181:6384.
    12. Cook, D.N. et al. (1995) Science 269:1583.
    13. Chintalacharuvu, S.R. et al. (2005) Immunol. Lett. 100:202.
    14. Ajuebor, M.N. et al. (2004) Eur. J. Immunol. 34:2907.
    15. Cocchi, F. et al. (1995) Science 270:1811.
    16. Zhang, N. et al. (2005) Proc. Natl. Acad. Sci. 102:4536.
  • Entrez Gene IDs:
    6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine)
  • Alternate Names:
    C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; Mip1a; MIP-1alpha; MIP1-alpha; Scya3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Macrophages confer survival signals via CCR1-dependent translational MCL-1 induction in chronic lymphocytic leukemia
    Authors: MH van Atteku, S Terpstra, E Slinger, M von Linder, PD Moerland, A Jongejan, AP Kater, E Eldering
    Oncogene, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Fc receptor-mediated phagocytosis in tissues as a potent mechanism for preventive and therapeutic HIV vaccine strategies
    Mucosal Immunol, 2016;9(6):1584-1595.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Serum amyloid A chemoattracts immature dendritic cells and indirectly provokes monocyte chemotaxis by induction of cooperating CC and CXC chemokines.
    Authors: Gouwy M, De Buck M, Portner N, Opdenakker G, Proost P, Struyf S, Van Damme J
    Eur J Immunol, 2015;45(1):101-12.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Increased levels of soluble interleukin-6 receptor and CCL3 in COPD sputum.
    Authors: Ravi A, Khurana S, Lemon J, Plumb J, Booth G, Healy L, Catley M, Vestbo J, Singh D
    Respir Res, 2014;15(0):103.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
    Authors: Badr G, Lefevre EA, Mohany M
    PLoS ONE, 2011;6(9):e23741.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. CXCR4 and CCR5 ligands cooperate in monocyte and lymphocyte migration and in inhibition of dual-tropic (R5/X4) HIV-1 infection.
    Authors: Gouwy M, Struyf S, Berghmans N, Vanormelingen C, Schols D, Van Damme J
    Eur. J. Immunol., 2011;41(4):963-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
  9. CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B.
    Authors: Leick M, Catusse J, Follo M, Nibbs RJ, Hartmann TN, Veelken H, Burger M
    Immunology, 2010;129(4):536-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
    Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
    J. Leukoc. Biol., 2008;84(4):1109-19.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Cognate CD4+ T-cell-dendritic cell interactions induce migration of immature dendritic cells through dissolution of their podosomes.
    Authors: Nobile C, Lind M, Miro F, Chemin K, Tourret M, Occhipinti G, Dogniaux S, Amigorena S, Hivroz C
    Blood, 2008;111(7):3579-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation.
    Authors: Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M
    Blood, 2008;112(3):493-503.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  14. Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
    Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A
    Int. J. Cancer, 2008;122(4):769-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Regulation of human LZIP expression by NF-kappaB and its involvement in monocyte cell migration induced by Lkn-1.
    Authors: Jang SW, Kim YS, Kim YR, Sung HJ, Ko J
    J. Biol. Chem., 2007;282(15):11092-100.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  17. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  18. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007;53(11):2010-2.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  19. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  20. Interaction between the CCR5 chemokine receptors and microbial HSP70.
    Authors: Whittall T, Wang Y, Younson J, Kelly C, Bergmeier L, Peters B, Singh M, Lehner T
    Eur. J. Immunol., 2006;36(9):2304-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  21. Changes in soluble factor-mediated CD8+ cell-derived antiviral activity in cynomolgus macaques infected with simian immunodeficiency virus SIVmac251: relationship to biological markers of progression.
    Authors: Dioszeghy V, Benlhassan-Chahour K, Delache B, Dereuddre-Bosquet N, Aubenque C, Gras G, Le Grand R, Vaslin B
    J. Virol., 2006;80(1):236-45.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  22. Novel antiviral activity of chemokines.
    Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
    Virology, 2006;350(2):484-92.
    Species: Virus
    Sample Type: Virus
    Application: Binding Assay
  23. MIP-1alpha (CCL3) is a downstream target of FGFR3 and RAS-MAPK signaling in multiple myeloma.
    Authors: Masih-Khan E, Trudel S, Heise C, Li Z, Paterson J, Nadeem V, Wei E, Roodman D, Claudio JO, Bergsagel PL, Stewart AK
    Blood, 2006;108(10):3465-71.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  24. Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.
    Authors: Chang WL, Baumgarth N, Yu D, Barry PA
    J. Virol., 2004;78(16):8720-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  25. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004;173(11):6592-602.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production.
    Authors: Tay SS, McCormack A, Rose ML
    Transplantation, 2004;78(7):987-94.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  27. Distribution, cellular localization and functional role of CCR2 chemokine receptors in adult rat brain.
    Authors: Banisadr G, Queraud-Lesaux F, Boutterin MC, Pelaprat D, Zalc B, Rostene W, Haour F, Parsadaniantz SM
    J. Neurochem., 2002;81(2):257-69.
    Species: Rat
    Sample Type: Whole Tissue
    Application: Binding Study
  28. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002;169(9):5244-51.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  29. Induction of inhibitory antibodies to the CCR5 chemokine receptor and their complementary role in preventing SIV infection in macaques.
    Authors: Lehner T, Wang Y, Doyle C, Tao L, Bergmeier LA, Mitchell E, Bogers WM, Heeney J, Kelly CG
    Eur. J. Immunol., 1999;29(8):2427-35.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Bioassay
  30. Neuropeptide Y induces potent migration of human immature dendritic cells and promotes a Th2 polarization.
    Authors: Buttari B, Profumo E, Domenici G, Tagliani A, Ippoliti F, Bonini S, Businaro R, Elenkov I, Rigano R
    FASEB J, 0;28(7):3038-49.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay - Ctrl
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FAQs

  1. What receptor does MIP-1 alpha bind to on TF1 cells?

    • MIP-1 alpha binds to CCR5 on TF1 cells.

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