Recombinant Human CD163 Fc Chimera Protein, CF

R&D Systems | Catalog # 11228-CD

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human CD163 Fc Chimera Protein (11228-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

AAH51281.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CD163 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD163 protein
Human CD163
(Gly46-Ser1050)
Accession # AAH51281.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly46

Predicted Molecular Mass

135 kDa

SDS-PAGE

145-160 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD163 Fc Chimera is immobilized at 2.00 μg/mL (100 μL/well), the concentration of human Haptoglobin/Hemoglobin (Hp/Hb) that produces 50% binding response is 20.0-250 ng/mL.

Scientific Data Images for Recombinant Human CD163 Fc Chimera Protein, CF

Recombinant Human CD163 Fc Chimera Protein SEC-MALS.

Recombinant Human CD163 Fc Chimera Protein (Catalog # 11228-CD) has a molecular weight (MW) of 314.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

Recombinant Human CD163 Fc Chimera Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Recombinant Human CD163 Fc Chimera Protein (Catalog # 11228-CD) is immobilized at 2.00 μg/mL (100 μL/well), the concentration of human Haptoglobin/Hemoglobin (Hp/Hb) that produces 50% binding response is 20.0-250 ng/mL.

Recombinant Human CD163 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human CD163 Fc Chimera Protein (Catalog # 11228-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 145-160 kDa and 290-320 kDa, respectively.

Formulation, Preparation, and Storage

11228-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD163

CD163, also known as M130, is a 130-160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1-3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 21 aa transmembrane segment, and an 85 aa cytoplasmic region. The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (2). While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). Expression of CD163 is constitutive, and induced by glucocorticoids, IL­10, IL­6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte­derived dendritic cells (1, 6-7). In addition to clearing Hb­Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF­like weak inducer of apoptosis) and can function as an erythroblast adhesion receptor (4, 8­, 10). The macrophage scavenger receptor CD163, which is upregulated in a number of inflammatory and malignant diseases, is a promising target on macrophages in anti-inflammatory and anti-cancer therapy in avoiding off-target effects on treatment of these diseases (11).

References

  1. Sulahian, T.H. et al. (2000) Cytokine 12:1312.
  2. Madsen, M. et al. (2004) J. Biol. Chem. 279:51561.
  3. Austermann, J. et al. (2022) Cells 11:1979.
  4. Kristiansen, M. et al. (2001) Nature 409:198.
  5. Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837.
  6. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
  7. Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414.
  8. Schaer, D.J. et al. (2006) Blood 107:373.
  9. Bover, L.C. et al. (2007) J. Immunol. 178:8183.
  10. Fabriek, B.O. et al. (2007) Blood 109:5223.
  11. Skytthe, M.K. et al. (2020) Int J. Mol. Sci. 21:5497.

Alternate Names

CD163, GHI/61, HbSR, M130, RM3/1

Entrez Gene IDs

9332 (Human); 93671 (Mouse); 312701 (Rat)

Gene Symbol

CD163

UniProt

AAH51281.1

Additional CD163 Products

Product Documents for Recombinant Human CD163 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD163 Fc Chimera Protein, CF

For research use only

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