Recombinant Human CD163 Fc Chimera Protein, CF Summary
Accession # AAH51281.1
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Measured by its binding ability in a functional ELISA. When Recombinant Human CD163 Fc Chimera Protein (Catalog # 11228-CD) is immobilized at 2.00 μg/mL (100 μL/well), the concentration of human Haptoglobin/Hemoglobin (Hp/Hb) that produces 50% binding response is 20.0-250 ng/mL.
2 μg/lane of Recombinant Human CD163 Fc Chimera Protein (Catalog # 11228-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 145-160 kDa and 290-320 kDa, respectively.
CD163, also known as M130, is a 130-160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1-3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 21 aa transmembrane segment, and an 85 aa cytoplasmic region. The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (2). While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). Expression of CD163 is constitutive, and induced by glucocorticoids, IL10, IL6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocytederived dendritic cells (1, 6-7). In addition to clearing HbHp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNFlike weak inducer of apoptosis) and can function as an erythroblast adhesion receptor (4, 8, 10). The macrophage scavenger receptor CD163, which is upregulated in a number of inflammatory and malignant diseases, is a promising target on macrophages in anti-inflammatory and anti-cancer therapy in avoiding off-target effects on treatment of these diseases (11).
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- Skytthe, M.K. et al. (2020) Int J. Mol. Sci. 21:5497.
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