Recombinant Human CD27/TNFRSF7 Fc Chimera Protein, CF Summary
Accession # P26842
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival (1). Mature human CD27 consists of a 172 amino acid (aa) extracellular domain (ECD) with three TNFR cysteine‑rich repeats, a 21 aa transmembrane segment, and a 48 aa cytoplasmic domain (2). Within the ECD, human CD27 shares 63% and 66% aa sequence identity with mouse and rat CD27, respectively. CD27 is expressed as a disulfide‑linked homodimer that carries N‑linked and O‑linked glycosylation (3, 4). Proteolytic cleavage of CD27 results in the shedding of a 28‑32 kDa fragment of the ECD (4). CD27 is weakly expressed on naïve T cells and NK cells and is up‑regulated upon cell activation (4, 5). It is also up‑regulated on activated germinal center B cells, plasma cells, and a subset of memory B cells (6, 7). CD27 binds to the transmembrane glycoprotein CD27 Ligand/CD70 which is expressed on activated B cells, activated T cells, and dendritic cells (1, 8, 9). This interaction contributes to the activation and survival of CD4+ helper T cells (8‑11), CD8+ effector T cells (12, 13), memory T cells (10), and NK cells (5, 14). Ligation of CD27 on B cells promotes germinal center formation and the expansion and affinity maturation of memory B cell responses (6, 15).
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- Jung, J. et al. (2000) Eur. J. Immunol. 30:2437.
- Goodwin, R.G. et al. (1993) Cell 73:447.
- Bowman, M.R. et al. (1994) J. Immunol. 152:1756.
- Hendriks, J. et al. (2000) Nat. Immunol. 1:433.
- van Oosterwijk, M.F. et al. (2007) Int. Immunol. 19:713.
- Rowley, T.F. and A. Al Shamkhani (2004) J. Immunol. 172:6039.
- Hendriks, J. et al. (2003) J. Exp. Med. 198:1369.
- Kelly, J.M. et al. (2002) Nat. Immunol. 3:83.
- Raman, V.S. et al. (2003) J. Immunol. 171:5876.
Citations for Recombinant Human CD27/TNFRSF7 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Integrating SpyCatcher/SpyTag covalent fusion technology into phage display workflows for rapid antibody discovery
Authors: JK Fierle, J Abram-Sali, M Brioschi, M deTiani, G Coukos, SM Dunn
Sci Rep, 2019-09-06;9(1):12815.
Sample Types: Recombinant Protein
ARGX-110, a highly potent antibody targeting CD70, eliminates tumors via both enhanced ADCC and immune checkpoint blockade.
Authors: Silence K, Dreier T, Moshir M, Ulrichts P, Gabriels S, Saunders M, Wajant H, Brouckaert P, Huyghe L, Van Hauwermeiren T, Thibault A, De Haard H
Sample Types: Whole Cells
Blockade of protease-activated receptors on T cells correlates with altered proteolysis of CD27 by gingipains of Porphyromonas gingivalis.
Authors: Yun LW, Decarlo AA, Hunter N
Clin. Exp. Immunol., 2007-11-01;150(2):217-29.
Applications: ELISA (Standard)
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