Recombinant Human CD27/TNFRSF7 Fc Chimera Protein, CF

  (2 citations)   
Product Details
Citations (2)
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  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit the proliferation of mouse T cells induced by CD27 Ligand and anti-CD3. The ED50 for this effect is 0.75-3 µg/mL in the presence of 10 µg/mL (100 µL/well) Recombinant Mouse CD27 Ligand/TNFSF7 (Catalog # 783-CL) and anti-CD3.
  • Source
    Mouse myeloma cell line, NS0-derived
    Human CD27
    (Thr21-Ile192)
    Accession # P26842
    EGRMD Human IgG1
    (Pro100-Lys330)
    6-His tag
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Thr21
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    46.5 kDa (monomer)
  • SDS-PAGE
    60-70 kDa, reducing conditions
382-CD
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD27/TNFRSF7
CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival (1). Mature human CD27 consists of a 172 amino acid (aa) extracellular domain (ECD) with three TNFR cysteine‑rich repeats, a 21 aa transmembrane segment, and a 48 aa cytoplasmic domain (2). Within the ECD, human CD27 shares 63% and 66% aa sequence identity with mouse and rat CD27, respectively. CD27 is expressed as a disulfide‑linked homodimer that carries N‑linked and O‑linked glycosylation (3, 4). Proteolytic cleavage of CD27 results in the shedding of a 28‑32 kDa fragment of the ECD (4). CD27 is weakly expressed on naïve T cells and NK cells and is up‑regulated upon cell activation (4, 5). It is also up‑regulated on activated germinal center B cells, plasma cells, and a subset of memory B cells (6, 7). CD27 binds to the transmembrane glycoprotein CD27 Ligand/CD70 which is expressed on activated B cells, activated T cells, and dendritic cells (1, 8, 9). This interaction contributes to the activation and survival of CD4+ helper T cells (8‑11), CD8+ effector T cells (12, 13), memory T cells (10), and NK cells (5, 14). Ligation of CD27 on B cells promotes germinal center formation and the expansion and affinity maturation of memory B cell responses (6, 15).
  • References:
    1. Nolte, M.A. et al. (2009) Immunol. Rev. 229:216.
    2. Camerini, D. et al. (1991) J. Immunol. 147:3165.
    3. van Lier, R.A. et al. (1987) J. Immunol. 139:1589.
    4. Loenen, W.A. et al. (1992) Eur. J. Immunol. 22:447.
    5. Takeda, K. et al. (2000) J. Immunol. 164:1741.
    6. Xiao, Y. et al. (2004) J. Immunol. 172:7432.
    7. Jung, J. et al. (2000) Eur. J. Immunol. 30:2437.
    8. Goodwin, R.G. et al. (1993) Cell 73:447.
    9. Bowman, M.R. et al. (1994) J. Immunol. 152:1756.
    10. Hendriks, J. et al. (2000) Nat. Immunol. 1:433.
    11. van Oosterwijk, M.F. et al. (2007) Int. Immunol. 19:713.
    12. Rowley, T.F. and A. Al Shamkhani (2004) J. Immunol. 172:6039.
    13. Hendriks, J. et al. (2003) J. Exp. Med. 198:1369.
    14. Kelly, J.M. et al. (2002) Nat. Immunol. 3:83.
    15. Raman, V.S. et al. (2003) J. Immunol. 171:5876.
  • Entrez Gene IDs:
    939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)
  • Alternate Names:
    CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. ARGX-110, a highly potent antibody targeting CD70, eliminates tumors via both enhanced ADCC and immune checkpoint blockade.
    Authors: Silence K, Dreier T, Moshir M, Ulrichts P, Gabriels S, Saunders M, Wajant H, Brouckaert P, Huyghe L, Van Hauwermeiren T, Thibault A, De Haard H
    MAbs, 2014;6(2):523-32.
  2. Blockade of protease-activated receptors on T cells correlates with altered proteolysis of CD27 by gingipains of Porphyromonas gingivalis.
    Authors: Yun LW, Decarlo AA, Hunter N
    Clin. Exp. Immunol., 2007;150(2):217-29.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard

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Primary Antibodies
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Recombinant Proteins
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Recombinant Human IgG1 Fc, CF

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Recombinant Mouse CD27 Ligand/TNFSF7 Protein, CF

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Recombinant Mouse CD27 Ligand/TNFSF7 Protein

BA 783-CL 2
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