Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged), CF

A New rhCD40 Ligand is Now Available! ~10x better activity; HEK293 expressed; HA tag!
New Product 6420-CL/CF.
(4 citations)   
  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using enriched human B cells in the presence of IL-4. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543. The ED50 for this effect is 2‑12 ng/mL in the presence of 10 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050) and 20 ng/mL of recombinant human IL-4.
  • Source
    E. coli-derived Glu108-Leu261, with an N-terminal Met and 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    17.7 kDa (monomer)
  • SDS-PAGE
    18 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2706-CL/CF
 
2706-CL
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and EDTA.
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged) (Catalog #
2706-CL/CF) stimulates cell proliferation using enriched B cells in the presence of
IL-4. The ED50 for this effect is 2-12 ng/mL in the presence of 10 μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050) and 20 ng/mL of Recombinant Human IL‑4 (Catalog #
204-IL).
1 μg/lane of Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.
Background: CD40 Ligand/TNFSF5

CD40 Ligand, also known as TNFSF, CD154, TRAP, and gp39, is a 34-39 kDa type II transmembrane glycoprotein that belongs to the TNF superfamily (1-3). Mature human CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 215 aa extracellular region (4, 5). The extracellular domain of human CD40 Ligand shares 74% and 76% aa sequence identity with mouse and rat CD40 Ligand, respectively. Similar to other TNF superfamily members, CD40 Ligand forms a bioactive homotrimer, both as membrane bound and soluble forms (6-9). The 18 kDa soluble form (aa 113-261) arises from proteolytic processing. Mutation and alternative splicing generate additional forms of CD40 Ligand that are often truncated or non-trimerizable (8). CD40 Ligand is expressed on platelets, as well as on activated T cells and B cells, basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds to CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial, and epithelial cells (10). The interaction of CD40 Ligand with CD40 initiates signaling in both CD40 and CD40 Ligand expressing cells (11).  CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (12, 13). CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS (14, 15). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (16, 17).

 

  • References:
    1. Zhang, G. (2004) Curr. Opin. Struct. Biol. 14:154.
    2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
    3. Quezada, S.A. et al. (2004) Annu. Rev. Immunol. 22:307.
    4. Graf, D. et al. (1992) Eur. J. Immunol. 22:3191.
    5. Hollenbaugh, D. et al. (1992) EMBO J. 11:4313.
    6. Khandekar, S.S. et al. (2001) Protein Expr. Purif. 23:301.
    7. Pietravalle, F. et al. (1996) J. Biol. Chem. 271:5965.
    8. Garber, E. et al. (1999) J. Biol. Chem. 274:33545.
    9. Vakkalanka, R.K. et al. (1999) Arthritis Rheum. 42:871.
    10. van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
    11. Eissner, G. et al. (2004) Cytokine Growth Factor. Rev. 15:353.
    12. Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
    13. Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
    14. Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
    15. Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
    16. Pamukcu, B. et al. (2011) Ann. Med. 43:331.
    17. Hassan, G.S. et al. (2012) Immunobiology 217:521.
  • Entrez Gene IDs:
    959 (Human); 21947 (Mouse); 84349 (Rat)
  • Alternate Names:
    CD154 antigen; CD154; CD40 antigen ligand; CD40 Ligand; CD40-L; CD40LG; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TNFSF5IMD3; TRAP; TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Posttranscriptional upregulation of p53 by reactive oxygen species in chronic lymphocytic leukemia
    Cancer Res, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. The TNF-family cytokine TL1A inhibits proliferation of human activated B cells.
    Authors: Cavallini C, Lovato O, Bertolaso A, Pacelli L, Zoratti E, Zanolin E, Krampera M, Zamo A, Tecchio C, Cassatella M, Pizzolo G, Scupoli M
    PLoS ONE, 2013;8(4):e60136.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Comparison of epithelial differentiation and immune regulatory properties of mesenchymal stromal cells derived from human lung and bone marrow.
    Authors: Ricciardi M, Malpeli G, Bifari F, Bassi G, Pacelli L, Nwabo Kamdje AH, Chilosi M, Krampera M
    PLoS ONE, 2012;7(5):e35639.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Overlapping human leukocyte antigen class I/II binding peptide vaccine for the treatment of patients with stage IV melanoma: evidence of systemic immune dysfunction.
    Authors: Celis E, Celis E
    Cancer, 2007;110(1):203-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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