Recombinant Human CD44s His-tag Protein, CF

Name: Recombinant Human CD44s His-tag Protein, CF
Brand: R&D Systems
SKU: 11428-CD
Price: 454 USD
Availability: InStock

R&D Systems | Catalog # 11428-CD

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

R&D Systems CHO-derived Recombinant Human CD44s His-tag Protein (11428-CD)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

NP_001001391.1

Applications

Bioactivity

View all CD44 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD44 protein
Gln21-Trp269 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln21

Predicted Molecular Mass

28 kDa

SDS-PAGE

71-80 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human CD44s His-tag (Catalog # 11428-CD) binds to Recombinant Human LSECtin/CLEC4G Protein (Catalog # 2947-CL) with a ED₅₀ of 20.0-120 ng/mL.

Scientific Data Images for Recombinant Human CD44s His-tag Protein, CF

Recombinant Human CD44s His-tag Protein SEC-MALS.

Recombinant Human CD44s His-tag (Catalog # 11428-CD) has a molecular weight (MW) of 48.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

Recombinant Human CD44s His-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Human CD44s His-tag Protein (Catalog #11428-CD) binds to Recombinant Human LSECtin/CLEC4G Protein (2947-CL) with a ED₅₀ of 20.0-120 ng/mL.

Recombinant Human CD44s His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human CD44s His-tag Protein (Catalog # 11428-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 71-80 kDa, under reducing conditions.

Formulation, Preparation, and Storage

11428-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD44

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1-3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 transcripts undergo complex alternative splicing, and, within the stem, ten variably spliced exons (v1-10 corresponding to exons 6-15; although human CD44 lacks v1/exon 6) produce multiple protein isoforms (1-4). The standard or hematopoietic form, CD44H, does not include the variable segments (1-4). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 4, 5). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N-terminal invariant portion of the ECD (aa 21-222), human CD44 shares 76%, 76%, 86%, 83% and 79% identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a "platform" for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (6) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (7-8). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta-like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (9-10). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 8).