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Recombinant Human CD47 Fc Chimera Avi-tag Protein, CF

R&D Systems | Catalog # AVI4670

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human CD47 Fc Chimera Avi-tag Protein (AVI4670)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q08722

Structure / Form

Disulfide-linked homodimer, Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity
View all CD47 Proteins and Enzymes »

Why choose R&D Systems Avi-tag CD47 Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD47 protein
Human CD47
(Gln19-Pro139)
Accession # Q08722		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag 
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln19 predicted

Predicted Molecular Mass

42 kDa

SDS-PAGE

60-70 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human SIRP alpha/CD172a Fc Chimera (Catalog # 4546-SA) is immobilized at 0.1 μg/mL (100 μL/well), the concentration of Recombinant Human CD47 Fc Chimera Avi-tag (Catalog # AVI4670) that produces 50% of the optimal binding response is 6‑48 ng/mL.

Scientific Data Images for Recombinant Human CD47 Fc Chimera Avi-tag Protein, CF

Recombinant Human CD47 Fc Chimera Avi-tag Protein Bioactivity

Recombinant Human CD47 Fc Chimera Avi-tag Protein Bioactivity

When Recombinant Human SIRP alpha/CD172a Fc Chimera (4546-SA) is immobilized at 0.1 µg/mL (100 µL/well), Recombinant Human CD47 Fc Chimera Avi-tag (Catalog # AVI4670) binds with an ED50 of 6-48 ng/mL.

Recombinant Human CD47 Fc Chimera Avi-tag Protein SDS-PAGE

Recombinant Human CD47 Fc Chimera Avi-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human CD47 Fc Chimera Avi-tag (Catalog # AVI4670) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-70 kDa and 120-140 kDa, respectively.
Surface plasmon resonance sensorgram of Avi-tag biotinylated human CD47 protein binding to human SIRP alpha

Binding of Human SIRP alpha to CD47 Fc Avi-tag Protein by surface plasmon resonance (SPR).

Avi-tag Biotinylated Recombinant Human CD47 Fc protein (Catalog # AVI4670) was immobilized on a Biacore Sensor Chip CM5 via the Avi-tag biotin, and binding to Recombinant Human SIRP alpha His protein (9378-SA) was measured at a concentration range between 6.85 nM and 3.51 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.134 µM.

Formulation, Preparation, and Storage

AVI4670
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD47

CD47, also known as Integrin-Associated Protein (IAP) and OA3, is a 40-60 kDa variably glycosylated atypical member of the immunoglobulin superfamily (1, 2). Human CD47 is an integral membrane protein that consists of a 123 amino acid (aa) extracellular domain (ECD) with a single Ig-like domain, five membrane-spanning regions with short intervening loops, and a 34 aa C-terminal cytoplasmic tail (3). Alternate splicing of human CD47 generates additional isoforms with deletions in the cytoplasmic tail (3). Within the N-terminal ECD, human CD47 shares 63% aa sequence identity with mouse and rat CD47. A portion of the N‑terminal ECD can by shed from smooth muscle cells by MMP-2-mediated proteolysis (4). The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells (5, 6). These interactions prevent macrophage-mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium (5-8). The CD47-SIRP alpha interaction is species specific, and this lack of cross-species interaction has been implicated in xenotransplantation rejection (16). CD47 associates in cis with Fas on T cells and enhances Fas‑mediated apoptosis; its ligation promotes T cell anergy and dampens Th1 immune responses (9-11). CD47 also associates in cis with Integrins  alpha 4 beta 1, alpha V beta 3, alpha 2b beta 3, and  alpha 2 beta 1 which can positively or negatively modulate Integrin‑mediated function (2, 12). In the vasculature, CD47 binding by Thrombospondin-1 inhibits the angiogenic and vasorelaxant effects of nitric oxide (2, 13, 14). On dendritic cells and myeloma cells, CD47 ligation by TSP-1 induces giant cell formation and osteoclast differentiation (15).

References

  1. Sarfati, M. et al. (2009) Curr. Drug Targ. 9:842.
  2. Isenberg, J.S. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:615.
  3. Campbell, I.G. et al. (1992) Cancer Res. 52:5416.
  4. Maile, L.A. et al. (2008) Mol. Endocrinol. 22:1226.
  5. Oldenborg, P.-A. et al. (2000) Science 288:2051.
  6. Liu, Y. et al. (2002) J. Biol. Chem. 277:10028.
  7. Stefanidakis, M. et al. (2008) Blood 112:1280.
  8. de Vries, H.E. et al. (2002) J. Immunol. 168:5832.
  9. Manna, P.P. et al. (2005) J. Biol. Chem. 280:29637.
  10. Avice, M.-N. et al. (2001) J. Immunol. 167:2459.
  11. Bouguermouh, S. et al. (2008) J. Immunol. 180:8073.
  12. Barazi, H.O. et al. (2002) J. Biol. Chem. 277:42859.
  13. Isenberg, J.S. et al. (2009) Nitric Oxide 21:52.
  14. Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.
  15. Kukreja, A. et al. (2009) Blood 114:3413.
  16. Wang H. et al. (2007) Blood 109:836.

Alternate Names

CD47, IAP, MER6, OA3

Entrez Gene IDs

961 (Human); 16423 (Mouse); 397042 (Porcine); 478552 (Canine); 102139846 (Cynomolgus Monkey)

Gene Symbol

CD47

UniProt

Q08722

Additional CD47 Products

Product Documents for Recombinant Human CD47 Fc Chimera Avi-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD47 Fc Chimera Avi-tag Protein, CF

For research use only

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