Recombinant Human CD8 alpha Fc Chimera Protein, CF

R&D Systems | Catalog # 10927-CD

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human CD8 alpha Fc Chimera Protein (10927-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P01732.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
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Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD8 protein
Human CD8 alpha
(Ser22-Asp182)
Accession # P01732.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser22

Predicted Molecular Mass

44 kDa

SDS-PAGE

54-60 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Human CD8 alpha Antibody (Catalog # MAB3803) is immobilized at 2.00 μg/mL, 100 μg/mL, Recombinant Human CD8 alpha alpha Fc Chimera binds with an ED50 of 1.50-15.0 ng/mL.

Scientific Data Images for Recombinant Human CD8 alpha Fc Chimera Protein, CF

Recombinant Human CD8 alpha Fc Chimera Protein Binding Activity.

When Human CD8a Antibody (MAB3803) is immobilized at 2 μg/mL, 100 μg/mL, Recombinant Human CD8a Fc Chimera Protein (Catalog # 10927-CD) binds with an ED50 of 1.50-15.0 ng/mL.

Recombinant Human CD8 alpha Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human CD8 alpha Fc Chimera Protein (Catalog # 10927-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-60 kDa and 110-120 kDa, respectively.

Formulation, Preparation, and Storage

10927-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD8

CD8, also known as Ly2 or Leu2, is a heterodimeric glycoprotein (alpha and beta subunits) that functions in conjunction with the T cell receptor in the recognition of MHC class I/peptide complexes (1, 2). CD8 alpha is expressed on double positive (CD4+ CD8+) thymocytes and mature CD8+ cytolytic T cells (CTL) (3-5), intraepithelial lymphocytes (IEL) (6), some gamma delta T cells (7), subsets of thymic and splenic dendritic cells (DC) (88), and peritoneal mast cells (9). It can form disulfide linked homodimers or heterodimers with CD8 beta (10). Thymic CD8+ DC express primarily alpha beta  heterodimers, while splenic CD8+ DC primarily express alpha alpha  homodimers (8). CD8a+ DC can present viral antigenic peptides in complex with MHC I and prime CTL responses (11). The approximately 35 kDa mature mouse CD8 alpha consists of a 169 amino acid (aa) extracellular domain (ECD) with one Iglike domain, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (12). Within the ECD, mouse CD8 alpha shares 49% and 64% aa sequence identity with human and rat CD8 alpha, respectively.

References

  1. Laugel, B. et al. (2011) J. Leukoc. Biol. 90:1089.
  2. Cole, D.K. et al. (2012) Immunology 137:139.
  3. Germain, R.N. (2002) Nat. Rev. Immunol. 2:309.
  4. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  5. Nakayama, K. et al. (1994) Science 263:1131.
  6. Wang, J. and J.R. Klein (1994) Science 265:1860.
  7. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
  8. Vremec, D. et al. (1992) J. Exp. Med. 176:47.
  9. Lin, T.J. et al. (1998) J. Immunol. 161:6265.
  10. Snow, P.M. and C. Terhorst (1983) J. Biol. Chem. 258:14675.
  11. Belz, G.T. et al. (2004) J. Immunol. 172:1996.
  12. Nakauchi, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:5126.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

UniProt

P01732.1

Additional CD8 Products

Product Documents for Recombinant Human CD8 alpha Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD8 alpha Fc Chimera Protein, CF

For research use only

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