Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1033123
View all CD8 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD8 alpha
Ser22-Val198
Accession # NP_741969.1

Specificity

Detects human CD8 alpha in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CD8 alpha Antibody

Detection of CD8 alpha in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD8 alpha Monoclonal Antibody (Catalog # MAB3803) or (B) Mouse IgG2A isotype control antibody (MAB003) followed by anti-Mouse IgG Phycoerythrin-conjugated secondary antibody (F0102B) and Mouse Anti-Human CD3 epsilon Allophycocyanin-conjugated Monoclonal Antibody (FAB100A). Staining was performed using our Staining Membrane-associated Proteins protocol.

Detection of CD8 alpha in HEK293 Human Cell Line Transfected with Cynomolgous Monkey CD8 alpha and eGFP by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with (A) cynomolgous monkey CD8 alpha or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human/Cynomolgous Monkey CD8 alpha Monoclonal Antibody (Catalog # MAB3803) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB003).Staining was done using our Staining Membrane-associated Proteins protocol.

CD8 alpha in Human PBMCs.

CD8 alpha was detected in immersion fixed Human peripheral blood mononuclear cells (PBMCs, positive staining) and HDLM‑2 human Hodgkin’s lymphoma cell line (negative control) using Mouse Anti-Human CD8 alpha Monoclonal Antibody (Catalog # MAB3803) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CD8 alpha in Human Lymph Node.

CD8 alpha was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human CD8 alpha Monoclonal Antibody (Catalog # MAB3803) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human CD8 alpha Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC lymphocytes and  HEK293 Human Cell Line Transfected with Cynomolgous Monkey CD8 alpha and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Human peripheral blood mononuclear cells (PBMCs)

Immunohistochemistry

0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node

Reviewed Applications

Read 1 review rated 5 using MAB3803 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD8

CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

UniProt

NP_741969.1

Additional CD8 Products

Product Documents for Human CD8 alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD8 alpha Antibody

For research use only

Customer Reviews for Human CD8 alpha Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human CD8 alpha Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human CD8 alpha Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Spleen tissue
    Species: Human
    Verified Customer | Posted 12/21/2021
    Human CD8 alpha Antibody MAB3803

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies