Recombinant Human CEACAM-8/CD66b Protein, CF Summary
Gln35-Asp320, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human Galectin-3 (Catalog # 1154-GA) is coated at 2 µg/mL, 100 µL/well, it binds to Recombinant CEACAM-8/CD66b (Catalog # 9639-CM) with an ED50 of 0.4-2.4 µg/mL.
Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM-8), also known as CGM6 and CD66b, is an approximately 95 kDa member of the human carcinoembryonic antigen (CEA) family that plays a role in cell adhesion, cell migration and pathogen binding (1, 2). CEACAM-8 is anchored to cells by a glycophosphatidylinositol (GPI) motif and is expressed exclusively on granulocytes (3, 4). Human CEACAM-8 is composed of a 34 amino acid (aa) signal peptide, a 29 aa propeptide segment which is removed in the mature form, and a 286 aa CEACAM-8 region containing one V-type and two C2-type Ig-like domains (5). A homologue for CEACAM-8 has not been identified in the mice or rats (1). Activation enhances surface expression of CEACAM-8 and is an activation marker in human neutrophils and eosinophils (1, 6, 7). Engagement of CEACAM-8 by mAb or galectin-3 activated a Src family kinase (lyn), and induced cellular adhesion, superoxide production, and degranulation of eosinophils (1). CEACAM-8 can form both homotypic, and heterotypic bonds with CEACAM-6 through interaction of the V-type Ig-like domains (7-9). Cross-linking of neutrophil CEACAM-8 augments cytokine secretion and beta2 integrin-mediated adhesion (10, 11).
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- Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
- Bonsor, D.A. et al. (2015) Proc. Natl. Acad. Sci. USA 112:13561.
- Tchoupa, T. et al. (2014) Cell. Commun. Signal. 12:27.
- Eades-Perner AM. et al. (1998) Blood 91:663.
- Schmidt T. (2012) J Leukoc Biol. 91:791.
- Kuroki, M. et al. (2001) J. Leukoc. Biol. 70:543.
- Yamanka T et al. (1996) Biochem Biophys Res Commun. 219:842.
- Schröder AK et al. (2006) Hum Immunol. 67:676.
- Skubitz KM et al. (1996) J Leukoc Biol. 60:106.
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