Recombinant Human CEACAM-8/CD66b Protein, CF

Catalog # Availability Size / Price Qty
9639-CM-050
Recombinant Human CEACAM-8/CD66b Protein Bioactivity
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Recombinant Human CEACAM-8/CD66b Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin‑3 (Catalog # 1154-GA) is coated at 2 μg/mL, 100 μL/well, it binds to Recombinant CEACAM‑8/CD66b with an ED50 of 0.4-2.4 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human CEACAM-8/CD66b protein
Gln35-Asp320, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Gln35
Predicted Molecular Mass
32 kDa
SDS-PAGE
67-77 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9639-CM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human CEACAM-8/CD66b Protein Bioactivity View Larger

When Recombinant Human Galectin-3 (Catalog # 1154-GA) is coated at 2 µg/mL, 100 µL/well, it binds to Recombinant CEACAM-8/CD66b (Catalog # 9639-CM) with an ED50 of 0.4-2.4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEACAM-8/CD66b

Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM-8), also known as CGM6 and CD66b, is an approximately 95 kDa member of the human carcinoembryonic antigen (CEA) family that plays a role in cell adhesion, cell migration and pathogen binding (1, 2). CEACAM-8 is anchored to cells by a glycophosphatidylinositol (GPI) motif and is expressed exclusively on granulocytes (3, 4). Human CEACAM-8 is composed of a 34 amino acid (aa) signal peptide, a 29 aa propeptide segment which is removed in the mature form, and a 286 aa CEACAM-8 region containing one V-type and two C2-type Ig-like domains (5). A homologue for CEACAM-8 has not been identified in the mice or rats (1). Activation enhances surface expression of CEACAM-8 and is an activation marker in human neutrophils and eosinophils (1, 6, 7). Engagement of CEACAM-8 by mAb or galectin-3 activated a Src family kinase (lyn), and induced cellular adhesion, superoxide production, and degranulation of eosinophils (1). CEACAM-8 can form both homotypic, and heterotypic bonds with CEACAM-6 through interaction of the V-type Ig-like domains (7-9). Cross-linking of neutrophil CEACAM-8 augments cytokine secretion and beta2 integrin-mediated adhesion (10, 11).

References
  1. Yoon, J. et al. (2007) J. Immunol. 179:8454.
  2. Feuk-Lagerstedt, E. et al. (1999) J. Immunol. 163:5592.
  3. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  4. Bonsor, D.A. et al. (2015) Proc. Natl. Acad. Sci. USA 112:13561.
  5. Tchoupa, T. et al. (2014) Cell. Commun. Signal. 12:27.
  6. Eades-Perner AM. et al. (1998) Blood 91:663.
  7. Schmidt T. (2012) J Leukoc Biol. 91:791.
  8. Kuroki, M. et al. (2001) J. Leukoc. Biol. 70:543.
  9. Yamanka T et al. (1996) Biochem Biophys Res Commun. 219:842.
  10. Schröder AK et al. (2006) Hum Immunol. 67:676.
  11. Skubitz KM et al. (1996) J Leukoc Biol. 60:106.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 8
Entrez Gene IDs
1088 (Human); 102138843 (Cynomolgus Monkey)
Alternate Names
Carcinoembryonic antigen CGM6; carcinoembryonic antigen gene family member 6; carcinoembryonic antigen-related cell adhesion molecule 8; CD66b antigen; CD66b; CD67 antigen; CD67; CEACAM8; CEACAM-8; CGM6; NCA-95; Non-specific cross-reacting antigen NCA-95

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