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Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

R&D Systems | Catalog # 342-CD

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc (342-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P10747

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CD28 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived CD28 protein
Human/Cynomolgus Monkey/Rhesus Macaque CD28
(Asn19-Pro152)
Accession # P10747		 DMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn19

Predicted Molecular Mass

42.2 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human/Cynomolgus Monkey/Rhesus Macaque CD28 Fc Chimera is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1) that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.

Reviewed Applications

Read 2 reviews rated 4.5 using 342-CD in the following applications:

Formulation, Preparation, and Storage

342-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD28

CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant co-stimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig
V-like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is up-regulated rapidly following T cell activation and CD28 ligation. Cell surface expression of mouse CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the
up-regulation of bcl-XL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation.

References

  1. Lenschow, D.J. et al. (1996) Annu. Rev. Immunol. 14:233.
  2. Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131.
  3. Ward, S.G. (1996) Biochem. J. 318:361.

Alternate Names

CD28

Entrez Gene IDs

940 (Human); 12487 (Mouse); 102146670 (Cynomolgus Monkey)

Gene Symbol

CD28

UniProt

P10747 (Human)

Additional CD28 Products

Product Documents for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

For research use only

Citations for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

Customer Reviews for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF (2)

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  • Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF
    Name: Anonymous
    Application: ELISA
    Verified Customer | Posted 09/12/2024
    Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF 342-CD
  • Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF
    Name: Melissa Kemp
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 09/12/2020
    Protein:protein interaction by Octet. Immobilize CD28-Fc and monitor interaction of CD80. Fitting KD ~2-3uM
    Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF 342-CD

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