Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

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342-CD-200

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Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human/Cynomolgus Monkey/Rhesus Macaque CD28 Fc Chimera is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1) that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.
Source
Mouse myeloma cell line, NS0-derived CD28 protein
Human/Cynomolgus Monkey/Rhesus Macaque CD28
(Asn19-Pro152)
Accession # P10747
DMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asn19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
42.2 kDa (monomer)
SDS-PAGE
60-70 kDa, reducing conditions

Product Datasheets

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342-CD

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

342-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CD28

CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant co-stimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig
V-like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is up-regulated rapidly following T cell activation and CD28 ligation. Cell surface expression of mouse CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the
up-regulation of bcl-XL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation.

References
  1. Lenschow, D.J. et al. (1996) Annu. Rev. Immunol. 14:233.
  2. Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131.
  3. Ward, S.G. (1996) Biochem. J. 318:361.
Entrez Gene IDs
940 (Human); 12487 (Mouse); 102146670 (Cynomolgus Monkey)
Alternate Names
CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44

Citations for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models
    Authors: A Hanson, K Elpek, E Duong, L Shallberg, M Fan, C Johnson, M Wallace, GR Mabry, S Sazinsky, L Pepper, CJ Shu, S Sathyanara, S Zuerndorfe, T Simpson, M Gostissa, M Briskin, D Law, J Michaelson, CJ Harvey
    PLoS ONE, 2020-09-24;15(9):e0239595.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Structural basis for cancer immunotherapy by the first-in-class checkpoint inhibitor ipilimumab
    Authors: UA Ramagopal, W Liu, SC Garrett-Th, JB Bonanno, Q Yan, M Srinivasan, SC Wong, A Bell, S Mankikar, VS Rangan, S Deshpande, AJ Korman, SC Almo
    Proc. Natl. Acad. Sci. U.S.A., 2017-05-08;0(0):.
    Applications: Bioassay
  3. Selective blockade of CD28-mediated T cell costimulation protects rhesus monkeys against acute fatal experimental autoimmune encephalomyelitis.
    Authors: Haanstra K, Dijkman K, Bashir N, Bauer J, Mary C, Poirier N, Baker P, Scobie L, 't Hart B, Vanhove B
    J Immunol, 2015-01-14;194(4):1454-66.
    Applications: Bioassay
  4. Cell-extrinsic CTLA4-mediated regulation of dendritic cell maturation depends on STAT3.
    Authors: Kowalczyk A, D'Souza C, Zhang L
    Eur J Immunol, 2014-01-22;44(4):1143-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm.
    Authors: Sakthivel P, Shively V, Kakoulidou M
    J. Intern. Med., 2007-04-01;261(4):399-407.
    Applications: ELISA (Standard)
  6. Autoantibodies against CD28 are associated with atopic diseases.
    Authors: Neuber K, Mahnss B, Hubner C, Gergely H, Weichenthal M
    Clin. Exp. Immunol., 2006-11-01;146(2):262-9.
    Species: Human
    Sample Types: N/A, Serum
    Applications: Bioassay, ELISA (Capture)
  7. Chimeric co-stimulatory molecules that selectively act through CD28 or CTLA-4 on human T cells.
    Authors: Lazetic S, Leong SR, Chang JC, Ong R, Dawes G, Punnonen J
    J. Biol. Chem., 2002-08-06;277(41):38660-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

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Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF
By Melissa Kemp on 09/12/2020
Application: Binding assay/Protein-protein interaction

Protein:protein interaction by Octet. Immobilize CD28-Fc and monitor interaction of CD80. Fitting KD ~2-3uM