Recombinant Human DC-SIGN/CD209 Protein, CF Summary
Accession # Q9NNX6
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Recombinant Human DC-SIGN (Catalog # 9136-DC) supports the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED50for this effect is 0.4-2 μg/mL.
Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca2+-dependent carbohydrate-binding lectin domain (2). Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms (3). DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4). DC-SIGN/CD209 binds to butyrophilin 2A1 and this interaction can be blocked by HIV pp120. DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (6, 7).
- Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
- Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
- Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
- Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
- Malcherek, G. et al. (2007) J. Immunol. 179:3804.
- Geijtenbeek, T.B. et al. (2000) Cell 100:575.
- Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.
Citations for Recombinant Human DC-SIGN/CD209 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma
Authors: J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani
PLoS ONE, 2019;14(7):e0219480.
Sample Types: Natural Protein
Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins
Authors: C Leclaire, K Lecointe, PA Gunning, S Tribolo, DW Kavanaugh, A Wittmann, D Latousakis, DA MacKenzie, N Kawasaki, N Juge
FASEB J., 2018;0(0):fj201700619R.
Sample Types: Recombinant Protein
Applications: Force Microscopy
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