Recombinant Human DC-SIGN/CD209 Protein, CF

    
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  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED50 for this effect is 0.4-2 μg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived human DC-SIGN/CD209 protein
    YPYDVPDYA Human DC-SIGN/CD209
    (Gln59-Ala404)
    Accession # Q9NNX6
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Tyr
  • Predicted Molecular Mass
    41 kDa
  • SDS-PAGE
    41-48 kDa, reducing conditions
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9136-DC
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 250 μg/mL in PBS.
 
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
Immobilized Recombinant Human
DC-SIGN (Catalog #
9136-DC) supports the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED50 for this effect is 0.4-2 μg/mL.
Background: DC-SIGN/CD209
Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca2+-dependent carbohydrate-binding lectin domain (2). Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms (3). DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4). DC-SIGN/CD209 binds to butyrophilin 2A1 and this interaction can be blocked by HIV pp120. DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (6, 7).
  • References:
    1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
    2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
    3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
    4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
    5. Malcherek, G. et al. (2007) J. Immunol. 179:3804.
    6. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
    7. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.
  • Long Name:
    Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1
  • Entrez Gene IDs:
    30835 (Human); 170786 (Mouse)
  • Alternate Names:
    CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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