Recombinant Human DNAM-1/CD226 Fc Chimera Protein, CF Summary
Accession # Q15762
|HIEGRMD||Human IgG1 |
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human DNAM-1 Fc Chimera (Catalog # 666-DN) is immobilized at 1 µg/mL, 100 µL/well, Biotinylated Recombinant Human CD155/PVR (Catalog # BT9174) binds with an ED50 of 3-18 ng/mL.
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (2-6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the forrmation of osteoclasts (7-10). Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung (11). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 12-14).
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- Morimoto, K. et al. (2007) Oncogene July 16 epub.
- Shibuya, K. et al. (1999) Immunity 11:615.
- Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
- Shibuya, A. et al. (1998) J. Immunol. 166:1671.
Citations for Recombinant Human DNAM-1/CD226 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity
Authors: J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang
Nature Communications, 2022;13(1):1606.
Sample Types: Whole Cells
Applications: Flow Cytometry
HIV-1 Nef-mediated downregulation of CD155 results in viral restriction by KIR2DL5+ NK cells
Authors: P Fittje, A Hœlzemer, WF Garcia-Bel, S Vollmers, A Niehrs, K Hagemann, G Martrus, C Körner, F Kirchhoff, D Sauter, M Altfeld
Sample Types: Recombinant Proteins
Association of elevated serum soluble CD226 levels with the disease activity and flares of systemic lupus erythematosus
Authors: M Nakano, M Ayano, K Kushimoto, S Kawano, K Higashioka, S Inokuchi, H Mitoma, Y Kimoto, M Akahoshi, N Ono, Y Arinobu, K Akashi, T Horiuchi, H Niiro
Scientific Reports, 2021;11(1):16162.
Sample Types: Serum
Applications: ELISA Standard
Ligand stimulation of CD155alpha inhibits cell adhesion and enhances cell migration in fibroblasts.
Authors: Oda T, Ohka S, Nomoto A
Biochem. Biophys. Res. Commun., 2004;319(4):1253-64.
Sample Types: Whole Cells
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