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Recombinant Human DNAM-1/CD226 Fc Chimera Protein, CF

Catalog #: 666-DN
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
666-DN-050
Recombinant Human DNAM-1/CD226 Fc Chimera Protein Binding Activity
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Product Details
Citations (4)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human DNAM-1/CD226 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human DNAM-1/CD226Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, it binds Biotinylated Recombinant Human CD155/PVR Fc Chimera (Catalog # BT9174) with an ED50 of 3-18 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human DNAM-1/CD226 protein
Human DNAM-1/CD226
(Glu19-Asn247)
Accession # Q15762		 HIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
Q15762
N-terminal Sequence
Analysis
Glu19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
53 kDa (monomer)
SDS-PAGE
75-80 kDa, reducing conditions

Complete Your Experiment

Reconstitution Buffer 1 (PBS)
Cat # RB01

Product Datasheets

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666-DN

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

666-DN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human DNAM-1/CD226 Fc Chimera Protein Binding Activity View Larger

When Recombinant Human DNAM-1 Fc Chimera (Catalog # 666-DN) is immobilized at 1 µg/mL, 100 µL/well, Biotinylated Recombinant Human CD155/PVR (Catalog # BT9174) binds with an ED50 of 3-18 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (2-6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the forrmation of osteoclasts (7-10). Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung (11). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 12-14).

References
  1. Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359.
  2. Shibuya, A. et al. (1996) Immunity 4:573.
  3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
  5. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
  6. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
  7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  8. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
  9. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
  10. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
  11. Morimoto, K. et al. (2007) Oncogene July 16 epub.
  12. Shibuya, K. et al. (1999) Immunity 11:615.
  13. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
  14. Shibuya, A. et al. (1998) J. Immunol. 166:1671.
Long Name
DNAX Accessory Molecule 1
Entrez Gene IDs
10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
Alternate Names
CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1

Citations for Recombinant Human DNAM-1/CD226 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity
    Authors: J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang
    Nature Communications, 2022;13(1):1606.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. HIV-1 Nef-mediated downregulation of CD155 results in viral restriction by KIR2DL5+ NK cells
    Authors: P Fittje, A Hœlzemer, WF Garcia-Bel, S Vollmers, A Niehrs, K Hagemann, G Martrus, C Körner, F Kirchhoff, D Sauter, M Altfeld
    Oncogene, 2022;18(6):e1010572.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  3. Association of elevated serum soluble CD226 levels with the disease activity and flares of systemic lupus erythematosus
    Authors: M Nakano, M Ayano, K Kushimoto, S Kawano, K Higashioka, S Inokuchi, H Mitoma, Y Kimoto, M Akahoshi, N Ono, Y Arinobu, K Akashi, T Horiuchi, H Niiro
    Scientific Reports, 2021;11(1):16162.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Standard
  4. Ligand stimulation of CD155alpha inhibits cell adhesion and enhances cell migration in fibroblasts.
    Authors: Oda T, Ohka S, Nomoto A
    Biochem. Biophys. Res. Commun., 2004;319(4):1253-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

RB01
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