Recombinant Human DPPIV/CD26 His-tag Protein, CF

R&D Systems | Catalog # 11244-SE

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human DPPIV/CD26 His-tag Protein (11244-SE)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

CAA43118.1

Applications

Enzyme Activity
View all DPPIV/CD26 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human DPPIV/CD26 protein
Asn29-Pro766 with a C-terminal DI and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn29

Predicted Molecular Mass

86.4 kDa

SDS-PAGE

96-108 kDa, under reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC).
The specific activity is >8500 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human DPPIV/CD26 His-tag Protein, CF

Recombinant Human DPPIV/CD26 His-tag Protein Enzyme Activity.

Recombinant Human DPPIV/CD26 His-tag Protein (Catalog # 11244-SE) is measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC).

Recombinant Human DPPIV/CD26 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human DPPIV/CD26 His-tag Protein (Catalog # 11244-SE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 96-108 kDa.

Formulation, Preparation, and Storage

11244-SE
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: DPPIV/CD26

Dipeptidyl peptidase 4 (DPPIV, also known as CD26) is an approximately 110 kDa serine exopeptidase that releases Xaa-Pro or Xaa-Ala dipeptides from the N‑terminus of oligo- and polypeptides. Mature human DPPIV consists of a 6 amino acid (aa) cytoplasmic tail, a 22 aa transmembrane segment, and a 738 aa extracellular domain (ECD) that contains the catalytic active site (1). DPPIV is expressed as a noncovalent homodimer on the surface of epithelial cells, endothelial cells, and activated lymphocytes, and it can be released by MMP mediated shedding (2). It regulates immune and endocrine function through the cleavage of multiple chemokines, growth factors, and peptide hormones (3,4). It cleaves a range of peptide hormones including Glucagon, Glucagon-like Peptides 1 and 2, GIP, GHRH, Procalcitonin, Neuropeptide Y, and Substance P (5). It is released from adipocytes and induces insulin resistance in adipocytes and skeletal muscle (6). DPPIV also cleaves many chemokines, resulting in altered chemotactic activity (7-10) or impacting chemokine blockade of HIV-1 cellular infectivity depending on the chemokine target (7,9,11). It cleaves human GM-CSF and IL-3 and reduces their ability to promote myeloid cell development (12). In addition to enzymatic cleavage functions, DPPIV interacts with adenosine deaminase on T cells and with caveolin-1 on antigen presenting cells (13), provides costimulatory proliferation and activation signals to both CD4+ and CD8+ T cells (13,14), and serves as a cell entry coreceptor for HIV and coronavirus MERS-CoV-2 (15,16). DPPIV inhibitors are approved for therapeutic treatment in type 2 diabetes (17) and are being explored for treatment of several additional conditions including autoimmune diseases (18).  

References

  1. Tanaka, T. et al. (1992) J. Immunol. 149:481.
  2. Rohrborn, D. et al. (2014) FEBS Lett. 588:3870.
  3. Klemann, C. et al. (2016) Clin. Exp. Immunol. 185:1.
  4. Mortier, A. et al. (2016) J. Leukoc. Biol. 99:955.
  5. Waumans, Y. et al. (2015) Front. Immunol. 6:387.
  6. Lamers, D. et al. (2011) Diabetes 60:1917.
  7. Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
  8. Proost, P. et al. (2001) Blood 98:3554.
  9. Ohtsuki, T. et al. (1998) FEBS Lett. 431:236.
  10. Barreira da Silva, R. et al. (2015) Nat. Immunol. 16:850.
  11. Guan, E. et al. (2002) J. Biol. Chem. 277:32348.
  12. Broxmeyer, H.E. et al. (2012) Nat. Med. 18:1786.
  13. Ohnuma, K. et al. (2007) J. Biol. Chem. 282:10117.
  14. Hatano, R. et al. (2013) Immunology 138:165.
  15. Callebaut, C. et al. (1993) Science 262:2045.
  16. Raj, V.S. et al. (2013) Nature 495:251.
  17. Makrilakis, K. (2019) Int. J. Environ. Res. Public Health 16:2720.
  18. Huang, J. et al. (2022) Front. Immunol. 13:830863.

Long Name

Dipeptidyl-peptidase IV

Alternate Names

CD26, DPP4

Entrez Gene IDs

1803 (Human); 13482 (Mouse); 397492 (Porcine); 102133935 (Cynomolgus Monkey)

Gene Symbol

DPP4

UniProt

CAA43118.1

Additional DPPIV/CD26 Products

Product Documents for Recombinant Human DPPIV/CD26 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human DPPIV/CD26 His-tag Protein, CF

For research use only

Citations for Recombinant Human DPPIV/CD26 His-tag Protein, CF

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Protocols

View specific protocols for Recombinant Human DPPIV/CD26 His-tag Protein, CF (11244-SE):

Materials
  • Assay Buffer: 25 mM Tris, pH 8.0
  • Recombinant Human DPPIV/CD26 His-tag (rhCD26) (Catalog # 11244-SE)
  • Substrate: H-Gly-Pro-AMC, 10 mM stock in DMSO
  • Black 96-well Plate
  • Fluorescent Plate Reader
  1. Dilute rhCD26 to 0.1 ng/μL in Assay Buffer. Minimize the number of dilution steps to obtain the best activity results.
  2. Dilute Substrate to 200 μM in Assay Buffer.
  3. Load into a plate 50 μL of 0.1 ng/μL rhCD26 and start the reaction by adding 50 μL of 200 μM Substrate. Include a Substrate Blank containing 50 μL of Assay Buffer and 50 μL of 200 μM Substrate.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

  *Adjusted for Substrate Blank

**Derived using calibration standard 7-amino, 4-Methyl Coumarin.
Per Well:
  • rhCD26: 0.005 μg
  • Substrate: 100 μM

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