Recombinant Human EGFR Isoform vIII Protein, CF
Recombinant Human EGFR Isoform vIII Protein, CF Summary
Leu25-Ser378 (Asn273Lys), with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The epidermal growth factor receptor (EGFR) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER2), ErbB3 (HER3), and ErbB4 (HER4). All family members are type I transmembrane glycoproteins that have an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain (1-3). Human EGFR shares 90% identity with mouse and rat EGFR. The amino acid 25-378 of EGFR encodes the most common extracellular domain truncation mutant, termed EGFRvIII. It has been proved that EGFRvIII is relevant in a number of cancer types. EGFRvIII is tumor specific, and has not been found in normal tissues. EGFRvIII is highly tumorigenic, and can cause aggressive tumor phenotype, with increased invasion, proliferation, angiogenesis, and evasion of apoptosis. This EGFRvIII-mediated enhanced tumourigenicity combined with the lack of EGFRvIII expression in normal tissue makes it an ideal therapeutic targets (4).
- Daly, R.J. (1999) Growth Factors, 16:255.
- Schlessinger, J. (2000) Cell. 103:211.
- Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.
- Kuan, C. T. et al. (2001) Endocrine-related cancer 8:83.
Citation for Recombinant Human EGFR Isoform vIII Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Visualizing Spatial and Stoichiometric Barriers to Bispecific T-cell Engager Efficacy
Authors: R You, J Artichoker, A Ray, H Gonzalez V, DA Rock, KP Conner, MF Krummel
Cancer Immunology Research, 2022;0(0):.
Sample Types: Serum
Applications: ELISA Capture
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