>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. When 5 x 104 cells/well are added to Recombinant Human EpCAM/TROP-1 and Human Fibronectin (0.5 μg/mL, Catalog # 1918-FN) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 0.4-2.4 μg/mL.
Human embryonic kidney cell, HEK293-derived Gln24-Lys265, with a C-terminal 6-His tag
Immobilized Recombinant Human EpCAM/TROP1 (Catalog # 9277-EP) enhances L Cells mousefibroblasts adhesion in the presence of Human Fibronectin (Catalog # 1918-FN). The ED50 for this effect is0.4-2.4 μg/mL.
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain (1). Human and mouse EpCAM share 82% aa sequence identity. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is expressed on basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia but not on normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (2). It is additionally expressed on undifferentiated embryonic stem cells, thymocytes, and dendritic cells (3-5). It is up-regulated on actively proliferating epithelial tissues, during adult liver regeneration, and on many epithelial cell-derived carcinomas (2, 6). EpCAM functions as a homophilic cell adhesion molecule (7). It associates into tetramers and forms complexes in cis with Claudin-7, CD44v6, TSPAN8, CD9, Integrin alpha 3, and Annexin A1 (8-11) that can interfere with cell adhesion (12, 13). Proteolytic cleavage of EpCAM releases multiple fragments from the ECD as well as a cytoplasmic fragment that can regulate gene transcription (14-16).
Strnad, J. et al. (1989) Cancer Res. 49:314.
Schnell, U. et al. (2013) Biochim. Biophys. Acta 1828:1989.
Ng, V.Y. et al. (2010) Stem Cells 28:29.
Nelson, A.J. et al. (1996) Eur. J. Immunol. 26:401.
Borkowski, T.A. et al. (1996) Eur. J. Immunol. 26:110.
de Boer, C.J. et al. (1999) J. Pathol. 188:201.
Litvinov, S.V. et al. (1994) J. Cell Biol. 125:437.
Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
Nubel, T. et al. (2009) Mol. Cancer Res. 7:285.
Kuhn, S. et al. (2007) Mol. Cancer Res. 5:553.
Schmidt, D.S. et al. (2004) Exp. Cell Res. 297:329.
Litvinov, S.V. et al. (1997) J. Cell Biol. 139:1337.
Gaiser, M.R. et al. (2012) Proc. Natl. Acad. Sci. USA 109:E889.
Have you used Recombinant Human EpCAM/TROP1 His-tag Protein, CF?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.