Recombinant Human EpCAM/TROP1 His-tag Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. When 5 x 104 cells/well are added to Recombinant Human EpCAM/TROP-1 and Human Fibronectin (0.5 μg/mL, Catalog # 1918-FN) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is typically 0.4-2.4 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Gln24-Lys265, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained. Gln24 inferred from enzymatic pyroglutamate treatment revealing Glu25
  • Predicted Molecular Mass
    28 kDa
  • SDS-PAGE
    30-42 kDa, reducing conditions
9277-EP
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Immobilized Recombinant Human EpCAM/TROP1 (Catalog #
9277-EP) enhances L Cells mousefibroblasts adhesion in the presence of Human Fibronectin (Catalog #
1918-FN). The ED50 for this effect is0.4-2.4 μg/mL.
Background: EpCAM/TROP1
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain (1). Human and mouse EpCAM share 82% aa sequence identity. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is expressed on basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia but not on normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (2). It is additionally expressed on undifferentiated embryonic stem cells, thymocytes, and dendritic cells (3-5). It is up-regulated on actively proliferating epithelial tissues, during adult liver regeneration, and on many epithelial cell-derived carcinomas (2, 6). EpCAM functions as a homophilic cell adhesion molecule (7). It associates into tetramers and forms complexes in cis with Claudin-7, CD44v6, TSPAN8, CD9, Integrin alpha 3, and Annexin A1 (8-11) that can interfere with cell adhesion (12, 13). Proteolytic cleavage of EpCAM releases multiple fragments from the ECD as well as a cytoplasmic fragment that can regulate gene transcription (14-16).
  • References:
    1. Strnad, J. et al. (1989) Cancer Res. 49:314.
    2. Schnell, U. et al. (2013) Biochim. Biophys. Acta 1828:1989.
    3. Ng, V.Y. et al. (2010) Stem Cells 28:29.
    4. Nelson, A.J. et al. (1996) Eur. J. Immunol. 26:401.
    5. Borkowski, T.A. et al. (1996) Eur. J. Immunol. 26:110.
    6. de Boer, C.J. et al. (1999) J. Pathol. 188:201.
    7. Litvinov, S.V. et al. (1994) J. Cell Biol. 125:437.
    8. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
    9. Nubel, T. et al. (2009) Mol. Cancer Res. 7:285.
    10. Kuhn, S. et al. (2007) Mol. Cancer Res. 5:553.
    11. Schmidt, D.S. et al. (2004) Exp. Cell Res. 297:329.
    12. Litvinov, S.V. et al. (1997) J. Cell Biol. 139:1337.
    13. Gaiser, M.R. et al. (2012) Proc. Natl. Acad. Sci. USA 109:E889.
    14. Schnell, U. et al. (2013) Biosci. Rep. 33:e00030.
    15. Schon, M.P. et al. (1993) Int. J. Cancer 55:988.
    16. Maetzel, D. et al. (2009) Nat. Cell Biol. 11:162.
  • Long Name:
    Epithelial Cell Adhesion Molecule
  • Entrez Gene IDs:
    4072 (Human); 17075 (Mouse)
  • Alternate Names:
    17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; EpCAM/TROP1; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP MAB050 17  
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His Tag Horseradish Peroxidase-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 1  
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His Tag Biotinylated Antibody

WB BAM050 1  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999
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