Recombinant Human Ephrin-B3 Fc Chimera Biotinylated Protein

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Recombinant Human Ephrin-B3 Fc Chimera Biotinylated Protein Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse EphB3 Fc Chimera at 2 µg/mL (100 µL/well) can bind biotinylated Recombinant Human Ephrin‑B3 Fc Chimera with a linear range of 1.3-80 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Ephrin-B3 protein
Human Ephrin-B3
Accession # Q15768
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
49.2 kDa (monomer)
58 kDa, reducing conditions

Product Datasheets

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Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Reconstitute at 100 μg/mL with sterile PBS.

Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Ephrin-B3

Ephrin-B3, also known as Elk-L3, LERK8, Eplg8, NLERK-2, and EFL6, is an approximately 50 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains (ECD) of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B3 preferentially interacts with receptors in the EphB family and also with EphA4. The binding of Ephrin-B3 to Eph proteins also triggers reverse signaling through Ephrin-B3 (1, 2). Mature human Ephrin-B3 consists of a 199 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 93 aa cytoplasmic domain (3, 4). Within the ECD, human Ephrin-B3 shares 96% and 97% aa sequence identity with mouse and rat Ephrin-B3, respectively. Ephrin-B3 is expressed on oligodendrocytes and neurons in the hippocampus and along the midline of the spinal cord (5-9). It is up-regulated in glioma and promotes tumor cell invasion and migration (10). Ephrin-B3 functions as a repulsive axon guidance molecule by inducing growth cone collapse, neurite retraction, and axon pruning (5-8). Its repulsive effect along the spinal cord midline restricts motor neuron axons to their ipsilateral sides, thereby maintaining the independence of voluntary left side/right side movements (8, 9). Ephrin-B3 plays a role in the regulation of excitatory synapse density and synaptic maturation (6, 11, 12). It also functions as a cellular receptor for Nipah virus (13) and can induce the migration of memory B cells (14).

  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Gale, N.W. et al. (1996) Oncogene 13:1343.
  4. Nicola, N.A. et al. (1996) Growth Factors 13:141.
  5. Benson, M.D. et al. (2005) Proc. Natl. Acad. Sci. USA 102:10694.
  6. Xu, N.-J. et al. (2011) Nat. Neurosci. 14:1421.
  7. Xu, N.-J. and M. Henkemeyer (2009) Nat. Neurosci. 12:268.
  8. Kullander, K. et al. (2001) Genes Dev. 15:877.
  9. Yokoyama, N. et al. (2001) Neuron 29:85.
  10. Nakada, M. et al. (2006) Cancer Res. 66:8492.
  11. McClelland, A.C. et al. (2010) Proc. Natl. Acad. Sci. USA 107:8830.
  12. Antion, M.D. et al. (2010) Mol. Cell. Neurosci. 45:378.
  13. Negrete, O.A. et al. (2006) PLoS Pathog. 2:e7.
  14. Holen, H.L. et al. (2011) Scand. J. Immunol. 74:144.
Entrez Gene IDs
1949 (Human); 13643 (Mouse)
Alternate Names
EFL-6; Efnb3; Elk-L3; EPH-related receptor tyrosine kinase ligand 8; Ephrin B3; EphrinB3; Ephrin-B3; Epl8; EPLG8EPH-related receptor transmembrane ligand ELK-L3; LERK-8; LERK8EFL6; NLERK-2

Citations for Recombinant Human Ephrin-B3 Fc Chimera Biotinylated Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Fc-Based Recombinant Henipavirus Vaccines Elicit Broad Neutralizing Antibody Responses in Mice
    Authors: Y Li, R Li, M Wang, Y Liu, Y Yin, X Zai, X Song, Y Chen, J Xu, W Chen
    Viruses, 2020-04-23;12(4):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  2. Sonic hedgehog pathway activation is associated with cetuximab resistance and EPHB3 receptor induction in colorectal cancer
    Authors: SH Park, MJ Jo, BR Kim, YA Jeong, YJ Na, JL Kim, S Jeong, HK Yun, DY Kim, BG Kim, SH Kang, SC Oh, DH Lee
    Theranostics, 2019-04-12;9(8):2235-2251.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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