Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of the EPO protein is required for biological activities in vivo (4). The mature human EPO protein shares 75% - 84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6-8). EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the erythropoietin receptor (EPO R) (8, 9). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce erythropoietin production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).
Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)
R&D Systems | Catalog # 287-TC
Key Product Details
- R&D Systems CHO-derived Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (287-TC)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
Ala28-Arg193
Purity
Endotoxin Level
Predicted Molecular Mass
SDS-PAGE
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 0.015-0.075 units/mL.
Reviewed Applications
Read 2 reviews rated 5 using 287-TC in the following applications:
Scientific Data Images for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)
Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) Protein SEC-MALS.
Recombinant human EPO (Catalog # 287-TC) has a molecular weight (MW) of 28.8 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Bioactivity of Erythropoietin / EPO Protein
Recombinant Human Erythropoietin / EPO (287-TC) stimulates cell proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner. The ED50 for this effect is 0.015-0.075 units/mL.
Affinity Measurements and Binding Kinetics of the Interaction between Erythropoietin R and Erythropoietin/EPO by SPR
Recombinant Human Erythropoietin R Fc Chimera (963-ER) was captured on a Sensor Chip Protein AGL, and binding to Recombinant Human Erythropoietin/EPO (287-TC) was measured at a concentration range between 0.488 pM and 1000 pM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.219 pM.Formulation, Preparation, and Storage
287-TC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 500 U/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 12 months from date of receipt at -20 to -70 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -70 °C under sterile conditions after reconstitution. |
Calculators
Background: Erythropoietin/EPO
References
- Koury, M.J. (2005) Exp. Hematol. 33:1263.
- Jacobs, K. et al. (1985) Nature 313:806.
- Wen, D. et al. (1993) Blood 82:1507.
- Tsuda E., et al. (1990) Eur. J. Biochem. 188:405.
- Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
- Eckardt, K.U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
- Sharples, E.J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
- Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
- Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
- Acs, G. et al. (2001) Cancer Res. 61:3561.
- Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
- Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
- Kertesz, N. et al. (2004) Dev. Biol. 276:101.
Alternate Names
Gene Symbol
UniProt
Additional Erythropoietin/EPO Products
Product Documents for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)
For research use only
Related Research Areas
Citations for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)
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Customer Reviews for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (2)
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Customer Images
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Application: Cell ProliferationVerified Customer | Posted 04/13/2018Using DRAQ-5 dye and GpA (erythroid cell marker) to determine levels of enucleation of differentiated cells in the Day 18 cultures. A large percentage of the cells have differentiated into mature reticulocytes/erythrocytes.
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Application: Cell migration/motilityVerified Customer | Posted 04/13/2018
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FAQs for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)
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Q: How does 287-TC differ from other Recombinant Human Erythropoietin/EPO proteins?
A: 287-TC is considered Tissue Culture Grade. This product is tested for its ability to support the proliferation assay using TF‑1 human erythroleukemic cells. If additional testing specifications are required, the 11264-TC product is recommended. 287-TC is an excellent economical alternative intended for customers using large quantities of EPO for cultures.
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Q: Recombinant Human EPO is listed in units. How does this translate to mass?
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Q: How does 287-TC differ from other Recombinant Human Erythropoietin/EPO proteins?
A: 287-TC is considered Tissue Culture Grade. This product is tested for its ability to support the proliferation assay using TF‑1 human erythroleukemic cells. If additional testing specifications are required, the 11264-TC product is recommended. 287-TC is an excellent economical alternative intended for customers using large quantities of EPO for cultures.
-
Q: Recombinant Human EPO is listed in units. How does this translate to mass?
Associated Pathways
Jak/STAT Signaling Pathway
