Recombinant Human Fc gamma RIIIB/CD16b (aa18-200) Protein

R&D Systems | Catalog # 9948-FC

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human Fc gamma RIIIB/CD16b (aa18-200) Protein (9948-FC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

NP_000561.3

Applications

Bioactivity
View all Fc gamma RIIIB/CD16b Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Fc gamma RIIIB/CD16b protein
Met18-Ser200, with a C-Terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met18

Predicted Molecular Mass

22 kDa

SDS-PAGE

37-50 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Fc gamma  RIIIB/CD16b is immobilized on a His Tag Antibody (Catalog # MAB050R) coated plate, it binds Biotinylated Human IgG. The concentration of Biotinylated Human IgG that produces 50% of the optimal binding response is 0.3-1.5 μg/mL.

Scientific Data Images for Recombinant Human Fc gamma RIIIB/CD16b (aa18-200) Protein

Recombinant Human Fc gamma RIIIB/CD16b (aa18-200) Protein Binding Activity

Recombinant Human Fc gamma RIIIB/CD16b (aa18-200) Protein Binding Activity

When Recombinant Human Fc gamma RIIIB/CD16b (Catalog # 9948-FC) is immobilized on a His Tag Antibody coated plate, Biotinylated Human IgG binds with an ED50 of 0.3-1.5 μg/mL.
Recombinant Human Fc gamma RIIIB/CD16b (aa18-200) Protein SDS-PAGE

Recombinant Human Fc gamma RIIIB/CD16b (aa18-200) Protein SDS-PAGE

2 μg/lane of Recombinant Human Fc gamma RIIIB was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 37 - 50 kDa.

Formulation, Preparation, and Storage

9948-FC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RIIIB/CD16b

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on the genetic organization and molecular structure of receptors for the Fc region of IgG (Fc gamma R), three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1-3). These receptors function in the activation or inhibition of immune responses. The activating-type receptor either has, or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular region of Fc gamma RIIIB share 62% and 59% sequence homology with the mouse and rat variants, respectively. The extracellular domains of human Fc gamma RIIIA and B share 97% amino acid sequence homology. Whereas Fc gamma RIIIA is expressed on most effector cells of the immune system including macrophage, monocyte, NK cells, mast cells, eosinophils, dendritic cells and Langerhans cells, Fc gamma RIIIB is selectively expressed in neutrophils and eosinophils. Signaling through Fc gamma RIIIA results in oxidative burst, cytokine release and phagocytosis by macrophages, antibody-dependent cellular cytotoxicity by natural killer cells and degranulation of mast cells. By contrast, Fc gamma RIIIB is a decoy receptor that binds IgG complexes without triggering activation. Soluble Fc gamma RIIIB has a regulatory role in inflammatory processes (4). It interacts with complement receptors CR3 and CR4 on monocytes to induce the production of pro-inflammatory cytokines.

References

  1. van de Winkel, J, and P. Capes (1993) Immunol. Today 14:215.
  2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Gauchat, G.J. et al. (1996) J. Immunol. 157:1184.

Long Name

Fc gamma Receptor III

Alternate Names

CD16b, FCG3, FCGR3B, FcgRIIIB, FcR-10, FCRIIIB, IGFR3

Entrez Gene IDs

2215 (Human); 14131 (Mouse)

Gene Symbol

FCGR3B

UniProt

NP_000561.3

Additional Fc gamma RIIIB/CD16b Products

Product Documents for Recombinant Human Fc gamma RIIIB/CD16b (aa18-200) Protein

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Product Specific Notices for Recombinant Human Fc gamma RIIIB/CD16b (aa18-200) Protein

For research use only

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FAQs for Recombinant Human Fc gamma RIIIB/CD16b (aa18-200) Protein

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  • Q: Which variants are the sequences expressed in your Recombinant Human Fc gamma RIIIB/CD16b proteins (catalogs # 1597-FC & 9948-FC)?

    A: The sequences for these proteins reflect the NA-2 variant. More information can be found in the following reference: https://pubmed.ncbi.nlm.nih.gov/2478590/

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