Recombinant Human Flt-3 Ligand Protein

(10 citations)   
  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is 0.2-1 ng/mL.
    The specific activity of Recombinant Human Flt-3 Ligand is approximately 1.2 x 103 U/μg, which is calibrated against recombinant human
    Flt-3 Ligand WHO Standard (NIBSC code: 96/532).
  • Source
    Mouse myeloma cell line, NS0-derived Thr27-Pro185
  • Accession #
  • N-terminal Sequence
    Analysis
    Thr27
  • Predicted Molecular Mass
    18 kDa
  • SDS-PAGE
    Multiple bands between 24 kDa and 28 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
308-FKN
 
308-FKN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute 5 µg vials at 50 µg/mL in sterile PBS. Reconstitute 25 µg or larger vials at 100 µg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human Flt-3 Ligand (Catalog # 308-FKN) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3. The ED50 for this effect is 0.2-1 ng/mL.
1 µg/lane of Recombinant Human Flt-3 Ligand was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at
24-28 kDa. Multiple bands are due to variable glycosylation.
Background: Flt-3 Ligand

Flt-3/Flk-2 Ligand (FL) is a hematopoietic cytokine whose activities are mediated by binding to the transmembrane glycoprotein Flt-3/Flk-2. Flt-3/Flk-2 was first discovered as a member of the class III subfamily of receptor tyrosine kinases (RTK) whose expression among hematopoietic cells was found to be restricted to highly enriched stem/progenitor cell populations. Additional class III RTKs include the receptors from SCF, M-CSF and PDGF. Not surprisingly, Flt-3/Flk-2 Ligand is also structurally related to M-CSF and SCF. All three cytokines have been shown to exist both as type I transmembrane proteins and as soluble proteins. The predominant human FL isoform is a transmembrane protein that can undergo proteolytic cleavage to generate a soluble form of the protein. An alternatively-spliced FL mRNA, encoding a soluble form of the human FL, has also been identified. FL is widely expressed in various human and mouse tissues. At the amino acid sequence level, human and mouse FL are approximately 72% identical and the two proteins exhibit cross-species activity. FL has been shown to synergize with a wide variety of hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors.

  • References:
    1. Lyman, S.D. (1995) International Journal of Hematology 62:63.
  • Long Name:
    fms-like Tyrosine Kinase 3 Ligand
  • Entrez Gene IDs:
    2323 (Human); 14256 (Mouse); 493796 (Feline)
  • Alternate Names:
    FL; Flt3 ligand; Flt-3 Ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
    Authors: Smith A, Bowers S, Stratman A, Davis G
    PLoS ONE, 2013;8(12):e85147.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells.
    Authors: Ichii, Michiko, Frank, Mark Bar, Iozzo, Renato V, Kincade, Paul W
    Blood, 2012;119(7):1683-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Optimal conditions for lentiviral transduction of engrafting human CD34+ cells.
    Authors: Uchida N, Hsieh MM, Hayakawa J, Madison C, Washington KN, Tisdale JF
    Gene Ther., 2011;18(11):1078-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Hematopoietic differentiation of induced pluripotent stem cells from patients with mucopolysaccharidosis type I (Hurler syndrome).
    Authors: Tolar J, Park I, Xia L, Lees C, Peacock B, Webber B, McElmurry R, Eide C, Orchard P, Kyba M, Osborn M, Lund T, Wagner J, Daley G, Blazar B
    Blood, 2011;117(3):839-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Wnt3a activates dormant c-Kit(-) bone marrow-derived cells with short-term multilineage hematopoietic reconstitution capacity.
    Authors: Trowbridge JJ, Guezguez B, Moon RT
    Stem Cells, 2010;28(8):1379-89.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. Mouse fetal and embryonic liver cells differentiate human umbilical cord blood progenitors into CD56-negative natural killer cell precursors in the absence of interleukin-15.
    Authors: McCullar V, Oostendorp R, Panoskaltsis-Mortari A, Yun G, Lutz CT, Wagner JE, Miller JS
    Exp. Hematol., 2008;36(5):598-608.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells.
    Authors: Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J
    Exp. Hematol., 2008;36(9):1121-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells.
    Authors: Gemelli C, Montanari M, Tenedini E, Zanocco Marani T, Vignudelli T, Siena M, Zini R, Salati S, Tagliafico E, Manfredini R, Grande A, Ferrari S
    Cell Death Differ., 2006;13(10):1686-96.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. BMP-6 inhibits human bone marrow B lymphopoiesis--upregulation of Id1 and Id3.
    Authors: Kersten C, Dosen G, Myklebust JH, Sivertsen EA, Hystad ME, Smeland EB, Rian E
    Exp. Hematol., 2006;34(1):72-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Prospective isolation of human clonogenic common myeloid progenitors.
    Authors: Manz MG, Miyamoto T, Akashi K, Weissman IL
    Proc. Natl. Acad. Sci. U.S.A., 2002;99(18):11872-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Animal Component Free Recombinant Proteins
Description Application Cat# Citations Images  

Recombinant Human Flt-3 Ligand, ACFP Protein

BA ACFP308
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Human Flt-3 Ligand Protein?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

  • Formulations

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Customers Who Viewed
This Item Also Viewed

Feedback

Customer Information