Frizzled-7 is a member of the Frizzled family of unconventional G-protein-coupled glycoprotein receptors for the Wnt signaling pathway (1 - 3). The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1, 2). Like other Frizzled family members, Frizzled-7 contains a divergent N-terminal signal peptide (amino acid (aa) 1 - 32), a highly conserved extracellular cysteine-rich domain (CRD, aa 44 - 169), a variable-length linker region (aa 170 ‑ 256), a seven-pass transmembrane region (aa 257 - 549), and a variable-length C-terminal cytoplasmic domain (aa 550 - 574) (1 - 3). The CRD, which comprises the binding site for Wnts and other ligands such as Syndecan 4 and fibronectin, spans about 130 amino acid residues and contains ten invariant cysteine residues (2, 3). Expressed alone, it can compete with native Frizzled to inhibit Wnt canonical signaling (4). Within aa 33 - 185, human Frizzled-7 shares ~99% aa identity with human, rat, canine and bovine Frizzled-7. Mature Frizzled-7 also shares 80% aa identity with Frizzled-1 and Frizzled-2. Roles for Frizzled-7 have been determined in both canonical Wnt/ beta -Catenin-mediated signaling and non‑canonical planar cell polarity and calcium pathways (1, 2, 4). During development, Frizzled-7 is expressed during gastrulation and in the fetal gut, kidney and lung where it is thought to influence tissue morphogenesis via non-canonical signaling pathways (3 - 5). In the adult, Frizzled-7 is expressed in skeletal muscle, especially in satellite cells that mediate muscle regeneration in response to Wnt-7a (3, 6). It is expressed in embryonic stem cells (ES), contributing to self-renewal signaling (7). It has been implicated in mesenchymal-to-epithelial transition in colorectal cancer (2, 8). Frizzled-7 mRNA has also been detected in adult heart and placenta (3).
Recombinant Human Frizzled-7 Fc Chimera Protein, CF
R&D Systems | Catalog # 6178-FZ
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Key Product Details
- R&D Systems CHO-derived Recombinant Human Frizzled-7 Fc Chimera Protein (6178-FZ)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
CHO
Accession Number
Structure / Form
Disulfide-linked homodimer
Applications
Bioactivity
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Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human Frizzled-7 protein
| Human Frizzled-7 (Gln33-Leu185) Accession # NP_003498 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gln33
Predicted Molecular Mass
43.3 kDa (monomer)
SDS-PAGE
55-66 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
In a 100 µL reaction mixture containing biotinylated rmWnt-5a at 100 ng/mL and rhFrizzled-7/Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled-7/Fc Chimera that produces 50% of the optimal binding response is found to be approximately 20-80 ng/mL.
Optimal concentrations should be determined by each laboratory for each application.
In a 100 µL reaction mixture containing biotinylated rmWnt-5a at 100 ng/mL and rhFrizzled-7/Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled-7/Fc Chimera that produces 50% of the optimal binding response is found to be approximately 20-80 ng/mL.
Optimal concentrations should be determined by each laboratory for each application.
Reviewed Applications
Read 2 reviews rated 4.5 using 6178-FZ in the following applications:
Formulation, Preparation, and Storage
6178-FZ
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: Frizzled-7
References
- Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
- Vincan, E. et al. (2007) Front. Biosci. 12:4558.
- Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
- Medina, A. et al. (2000) Mech. Dev. 92:227.
- Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
- Le Grand, F. et al. (2009) Cell Stem Cell 4:535.
- Melchior, K. et al. (2008) Biol. Chem. 389:897.
- Vincan, E. et al. (2007) Oncogene 26:2340.
Alternate Names
Frizzled7, FZD7
Gene Symbol
FZD7
UniProt
Additional Frizzled-7 Products
Product Documents for Recombinant Human Frizzled-7 Fc Chimera Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Frizzled-7 Fc Chimera Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human Frizzled-7 Fc Chimera Protein, CF
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Customer Reviews for Recombinant Human Frizzled-7 Fc Chimera Protein, CF (2)
4.5 out of 5
2 Customer Ratings
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Associated Pathways
Wnt Signaling Pathways: beta-Catenin-dependent Wnt Signaling
