Recombinant Human Furin Protein, CF

R&D Systems | Catalog # 1503-SE

Soon to be discontinued.
R&D Systems
Discontinued Product
1503-SE has been discontinued. An alternative/replacement product is available: 11338-SE. View all Furin products.

Key Product Details

Source

NS0

Accession Number

P09958

Structure / Form

Mature

Applications

Enzyme Activity
View all Furin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Furin protein
Asp108-Glu715, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp108 & Asp131

Predicted Molecular Mass

67 kDa

SDS-PAGE

58-85 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate pERTKR-AMC (Catalog # ES013).
The specific activity is >125 pmol/min/µg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 5 using 1503-SE in the following applications:

Scientific Data Images for Recombinant Human Furin Protein, CF

Recombinant Human Furin Protein Enzyme Activity

Recombinant Human Furin Protein Enzyme Activity

Recombinant Human Furin (1503-SE) is measured by its ability to cleave the fluorogenic peptide substrate pERTKR-AMC (ES013).

Formulation, Preparation, and Storage

1503-SE
Formulation Supplied as a 0.2 μm filtered solution in Tris, CaCl2, NaCl, Brij-35 and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Furin

Furin is a member of the proprotein convertase (PC) family, which belongs to the subtilisin superfamily of serine protease (1-3). As a cellular protease, Furin processes a variety of proproteins in secretory pathway compartments by cleaving after Arg-Xaa-Lys/Arg-Arg-like motifs, which usually reside at the end of the pro regions of these proproteins. Examples of the proprotein substrates are growth factors and receptors, extracellular matrix proteins, and other proteases. Furin has an essential role in embryogenesis and homeostasis and is implicated in various pathologies such as cancer, neurodegenerative diseases and anthrax. It is synthesized as a 794 amino acid type I transmembrane protein precursor with a signal peptide (residues 1-24), a pro region (residues 25-107), which play a crucial role in the folding, activation and transport of Furin, and a mature chain (residues 108-794) (1-3). The mature chain consists of the subtilisin-like catalytic domain, a P domain, which is essential for enzyme activity and the modulation of pH and calcium requirements, and a cytoplasmic domain, which controls the localization and sorting of Furin in the trans-Golgi network/endosomal system. The purified recombinant human Furin (residues 108-715) corresponds to the mature enzyme terminated before the transmembrane domain.

References

  1. Van den Ouweland, A.M. et al. (1990) Nucleic Acids Res. 18:664.
  2. Barr, P.J. et al. (1991) DNA Cell Biol. 10:319.
  3. Thomas, G. (2002) Nature Rev. Mol. Cell Biol. 3:753.

Alternate Names

FUR, PACE, PCSK3, SPC1

Entrez Gene IDs

5045 (Human); 18550 (Mouse)

Gene Symbol

FURIN

UniProt

P09958

Additional Furin Products

Product Documents for Recombinant Human Furin Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Furin Protein, CF

For research use only

Citations for Recombinant Human Furin Protein, CF

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    Application: In vivo study
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Protocols

View specific protocols for Recombinant Human Furin Protein, CF (1503-SE):

Materials
  • Assay Buffer:  25 mM Tris, 1 mM CaCl2, 0.5% (w/v) Brij-35, pH 9.0
  • Recombinant Human Furin (rhFurin) (Catalog # 1503-SE)
  • Substrate: p-Glu-Arg-Thr-Lys-Arg-AMC (Catalog # ES013)
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhFurin to 4 µg/mL in Assay Buffer.
  2. Dilute Substrate to 100 µM in Assay Buffer.
  3. Load into a black well plate 50 µL of 4 µg/mL of rhFurin, and start the reaction by adding 50 µL of 100 µM Substrate.  Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 100 µM Substrate.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank

     **Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891).

Per Well:

  • rhFurin: 0.2 µg
  • Substrate: 50 µM

























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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail