Recombinant Human Glypican 1 Protein, CF

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4519-GP-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (6)
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Recombinant Human Glypican 1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhGlypican 1 at 3 µg/mL (100 µL/well) will bind rhFGF-basic with an apparent KD < 1 nM.
Source
Mouse myeloma cell line, NS0-derived human Glypican 1 protein
Asp24-Ser530, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Asp24
Predicted Molecular Mass
56.8 kDa
SDS-PAGE
60 kDa, reducing conditions

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4519-GP

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4519-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Glypican 1

The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked proteoglycans that play a key role in growth factor signaling (1, 2, 3, 4). There are six known mammalian Glypicans. They all share a common-sized protein core of 60 - 70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Human Glypican 1 (GPC-1) is synthesized as a 558 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 507 aa mature segment, and a 28 aa C-terminal prosegment (6, 7). There are two potential N-linked and four potential O-linked sites for glycosylation or glycanation. There are potentially two heparan sulfate (HS) modifications on GPC-1 that could contribute to a native molecular weight of approximately 200 kDa (7, 8, 9). Mature human GPC-1 shares 91% aa identity with mature mouse GPC-1. There are two potential splice variants of human GPC-1. Both show an alternate start site at Met73, while one has an additional 65 aa substitution for the C-terminal 264 amino acids (10, 11). Cells known to express GPC-1 include neurons, smooth and skeletal muscle cells, keratinocytes, osteoblasts, Schwann cells, immature dendritic cells, and tumor, plus tumor-associated vascular endothelial cells (8, 9, 12 - 15). The function of GPC-1 is complex and varied. As a proteoglycan, it appears to make use of its HS adduct to impact select growth factor activity (16). This is accomplished by having juxtramembrane HS attachment sites, and a flexible, GPI-linkage (17). Data suggests GPC-1 and sulfation enzymes may collaborate to regulate FGF signaling. HS modules that are rich in 2-O- and 6-O- sulfate upregulate FGF-2 activation of FGFR1c (18). Similarly, FGF-1 requires both 2-O- and 6-O-sulfation to bind to FGFR2c and 3c. By contract, FGF-1 requires no sulfation to bind to FGFR2b, and FGF-8b needs only 6-O-sulfation to activate FGFR3c. Thus, many FGF receptor isoform specific effects may be attributed to an interaction between Glypican family members and the cell sulfation system (19).

References
  1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241.
  2. Fransson, L-A. et al. (2004) Cell. Mol. Life Sci. 61:1016.
  3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.
  4. Lamoureux, F. et al. (2007) BioEssays 29:758.
  5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
  6. GenBank Accession # P35052.
  7. David, G. et al. (1990) J. Cell Biol. 111:3165.
  8. Lories, V. et al. (1992) J. Biol. Chem. 267:1116.
  9. Lories, V. et al. (1989) J. Biol. Chem. 264:7009.
  10. GenBank Accession # EAW71184.
  11. GenBank Accession # EAW71183.
  12. Chernousov, M.A. et al. (2006) J. Neurosci. 26:508.
  13. Wegrowski, Y. et al. (2006) Clin. Exp. Immunol. 144:485.
  14. Qiao, D. et al. (2003) J. Biol. Chem. 278:16045.
  15. Kayed, H. et al. (2006) Int. J. Oncol. 29:1139.
  16. Selleck, S.B. (2006) SciSTKE, April 4:pe17.
  17. Qiao, D. et al. (2003) J. Biol. Chem. 278:16045.
  18. Su, G. et al. (2006) Am. J. Pathol. 168:2014.
  19. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
Entrez Gene IDs
2817 (Human); 14733 (Mouse); 58920 (Rat)
Alternate Names
FLJ38078; Glypican 1; glypican proteoglycan 1; glypican; glypican-1; GPC1

Citations for Recombinant Human Glypican 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Assessing the activity of antibodies conjugated to upconversion nanoparticles for immunolabeling
    Authors: Y Cao, J Wu, X Zheng, Y Lu, JA Piper, Y Lu, NH Packer
    Analytica chimica acta, 2022;1209(0):339863.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  2. Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle
    Authors: C Colin-Pier, V Untereiner, GD Sockalingu, N Berthélémy, L Danoux, V Bardey, S Mine, C Jeanmaire, L Ramont, S Brézillon
    Biomolecules, 2021;11(2):.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Imaging
  3. Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases
    Authors: AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano
    Sci Rep, 2020;10(1):12912.
    Species: Snake
    Sample Types: Protein
    Applications: Bioassay
  4. Anti-glypican-1 antibody-drug conjugate is a potential therapy against pancreatic cancer
    Authors: T Nishigaki, T Takahashi, S Serada, M Fujimoto, T Ohkawara, H Hara, T Sugase, T Otsuru, Y Saito, S Tsujii, T Nomura, K Tanaka, Y Miyazaki, T Makino, Y Kurokawa, K Nakajima, H Eguchi, M Yamasaki, M Mori, Y Doki, T Naka
    Br. J. Cancer, 2020;122(9):1333-1341.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Antibody Production
  5. A bispecific T cell engager targeting Glypican-1 redirects T cell cytolytic activity to kill prostate cancer cells
    Authors: ME Lund, CB Howard, KJ Thurecht, DH Campbell, SM Mahler, BJ Walsh
    BMC Cancer, 2020;20(1):1214.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  6. Epitope mapping by a Wnt-blocking antibody: evidence of the Wnt binding domain in heparan sulfate
    Sci Rep, 2016;6(0):26245.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA (Standard)

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