Recombinant Human gp130 Protein

Formulations:
Catalog # Availability Size / Price Qty
228-GP-010
228-GP-050
Product Details
Citations (12)
FAQs
Supplemental Products
Reviews (1)

Recombinant Human gp130 Protein Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-6-dependent proliferation of the 7TD1 mouse hybridoma cells in the presence of Recombinant Human IL-6 R alpha (Catalog # 227-SR). The ED50 for this effect is 0.5-2 µg/mL in the presence of 4 μg/mL recombinant human (rh) IL‑6 R alpha and 25 pg/mL rhIL-6.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human gp130 protein
Leu24-Glu619 (Glu619Asp)
Accession #
N-terminal Sequence
Analysis
Leu24
Predicted Molecular Mass
68 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

228-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

228-GP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: gp130

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. At the present time, the in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

References
  1. Narazaki, M. et al. (1993) Blood 82:1120.
  2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
Long Name
Glycoprotein 130
Entrez Gene IDs
3572 (Human); 16195 (Mouse); 25205 (Rat)
Alternate Names
CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha

Citations for Recombinant Human gp130 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Bmi1 Regulates I?B? Degradation via Association with the SCF Complex
    Authors: Y Okuyama, Y Tanaka, JJ Jiang, D Kamimura, A Nakamura, M Ota, T Ohki, D Higo, H Ogura, N Ishii, T Atsumi, M Murakami
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Soluble IL-6R expressed by myeloid cells reduces tumor-specific Th1 differentation and drives tumor progression
    Authors: H Tsukamoto, K Fujieda, M Hirayama, T Ikeda, A Yuno, K Matsumura, D Fukuma, K Araki, H Mizuta, H Nakayama, S Senju, Y Nishimura
    Cancer Res, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Discovery of a junctional epitope antibody that stabilizes IL-6 and gp80 protein:protein interaction and modulates its downstream signaling
    Authors: R Adams, RJ Burnley, CR Valenzano, O Qureshi, C Doyle, S Lumb, M Del Carmen, R Griffin, D McMillan, RD Taylor, C Meier, P Mori, LM Griffin, U Wernery, J Kinne, S Rapecki, TS Baker, AD Lawson, M Wright, A Ettorre
    Sci Rep, 2017;7(0):37716.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  4. Involvement of Spinal IL-6 Trans-Signaling in the Induction of Hyperexcitability of Deep Dorsal Horn Neurons by Spinal Tumor Necrosis Factor-Alpha
    J Neurosci, 2016;36(38):9782-91.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Breakpoint Cluster Region-Mediated Inflammation Is Dependent on Casein Kinase II
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. IL-6 trans-signaling is another pathway to upregulate Osteopontin
    Cytokine, 2016;90(0):88-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. The soluble interleukin-6 receptor is a mediator of hematopoietic and skeletal actions of parathyroid hormone.
    Authors: Cho S, Pirih F, Koh A, Michalski M, Eber M, Ritchie K, Sinder B, Oh S, Al-Dujaili S, Lee J, Kozloff K, Danciu T, Wronski T, McCauley L
    J Biol Chem, 2013;288(10):6814-25.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. A unique loop structure in oncostatin M determines binding affinity toward oncostatin M receptor and leukemia inhibitory factor receptor.
    Authors: Chollangi, Srinivas, Mather, Timothy, Rodgers, Karla K, Ash, John D
    J Biol Chem, 2012;287(39):32848-59.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA (Capture)
  9. Epigallocatechin-3-gallate inhibits IL-6 synthesis and suppresses transsignaling by enhancing soluble gp130 production.
    Authors: Ahmed S, Marotte H, Kwan K, Ruth JH, Campbell PL, Rabquer BJ, Pakozdi A, Koch AE
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(38):14692-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Functional characterization of a soluble gp130 isoform and its therapeutic capacity in an experimental model of inflammatory arthritis.
    Authors: Richards PJ, Nowell MA, Horiuchi S, McLoughlin RM, Fielding CA, Grau S, Yamamoto N, Ehrmann M, Rose-John S, Williams AS, Topley N, Jones SA
    Arthritis Rheum., 2006;54(5):1662-72.
    Species: Human
    Sample Types: Serum
    Applications: ELISA (Capture)
  11. Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells.
    Authors: Erices A, Conget P, Rojas C, Minguell JJ
    Exp. Cell Res., 2002;280(1):24-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
    Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
    Immunity, 2001;14(6):705-14.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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Recombinant Human gp130 Protein
By Anonymous on 12/01/2017
Application: Apoptosis assay