Recombinant Human Growth Hormone (GH) Protein

(6 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using Nb2‑11 rat lymphoma cells. Gout, P.W. et al. (1980) Cancer Res. 40:2433. The ED50 for this effect is 0.025-0.1 ng/mL.
  • Source
    E. coli-derived Phe27-Phe217, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    22 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1067-GH
 
1067-GH/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Growth Hormone

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretary granules. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones - GH-releasing hormone (GHRH) and somatostatin (SST) - as well as by signals from the periphery - ghrelin (3) and leptin (4). The human GH cDNA encodes a 217 amino acid (aa) residue precursor protein with a 26 aa putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified (5).

Human GH is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor, which is present in many cell types (1, 2). GH stimulates the liver and other tissues to produce IGF-1, which regulates growth and metabolism. GH has also been shown to have direct effects on growth that is independent of IGF-1. GH, directly or indirectly via IGF-1, can act on B cells, T cells, NK cells, macrophages and neutrophils to exert immunomodulatory activities (6). In addition, GH can act directly on various cell types to induce lipolysis, lactation, amino acid uptake and protein synthesis (1, 2, 6).

  • References:
    1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
    2. Le Roith, D. et al. (2001) Endocrine Rev., 22:53.
    3. Kojima, K. et al. (1999) Nature, 402:656.
    4. Tannenbaum, G. et al. (1998) Endocrinol. 139:3871.
    5. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
  • Entrez Gene IDs:
    2688 (Human)
  • Alternate Names:
    GH1; GHIGHD1B; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; Growth Hormone; hGH-N; Somatotropin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Growth hormone is permissive for neoplastic colon growth
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-alpha, and IL-1beta.
    Authors: Zhao Y, Xiao X, Frank S, Lin H, Xia Y
    Am J Physiol Endocrinol Metab, 2014;307(2):E186-98.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Growth hormone is secreted by normal breast epithelium upon progesterone stimulation and increases proliferation of stem/progenitor cells.
    Authors: Lombardi S, Honeth G, Ginestier C, Shinomiya I, Marlow R, Buchupalli B, Gazinska P, Brown J, Catchpole S, Liu S, Barkan A, Wicha M, Purushotham A, Burchell J, Pinder S, Dontu G
    Stem Cell Reports, 2014;2(6):780-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Insulin-like growth factor 1 and 2 (IGF1, IGF2) expression in human microglia: differential regulation by inflammatory mediators.
    Authors: Suh H, Zhao M, Derico L, Choi N, Lee S
    J Neuroinflammation, 2013;10(0):37.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes.
    Authors: Krejci P, Prochazkova J, Bryja V, Jelinkova P, Pejchalova K, Kozubik A, Thompson LM, Wilcox WR
    Cell. Signal., 2009;21(1):151-60.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  6. Growth hormone promotes lymphangiogenesis.
    Authors: Banziger-Tobler NE, Halin C, Kajiya K, Detmar M
    Am. J. Pathol., 2008;173(2):586-97.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

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