Recombinant Human Growth Hormone R (GHR) Fc Chimera, CF

Catalog # Availability Size / Price Qty
1210-GR-050

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Recombinant Human Growth Hormone R (GHR) Fc Chimera, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit GH-induced proliferation of Nb2‑11 rat lymphoma cells. Gout, P.W. et al. (1980) Cancer Res. 40:2433. The ED50 for this effect is 0.4-2.0 ng/mL in the presence of 0.2 ng/mL of rhGH.
Source
Mouse myeloma cell line, NS0-derived human Growth Hormone R/GHR protein
Human GHR
(Ala27-Tyr264)
Accession # P10912
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ala27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
54 kDa (monomer)
SDS-PAGE
75-90 kDa, reducing conditions

Product Datasheets

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1210-GR

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1210-GR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Growth Hormone R/GHR

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I, to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Human GHR cDNA encodes a 638 amino acid (aa) residue type I transmembrane protein with an 18 aa putative signal peptide, a 246 aa extracellular domain, a 24 aa transmembrane domain and a 350 aa cytoplasmic domain (5). At least two alternatively spliced isoforms of human GHR, lacking the sequence encoded by exon 3, or lacking most of the cytoplasmic domain, also exist (6, 7). Soluble GH-binding proteins corresponding to extracellular domain of the transmembrane proteins can be generated from the membrane proteins (8). Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (9). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (8, 10).

References
  1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
  2. Le Roith, D. et al. (2001) Endocrine Rev. 22:53.
  3. Clark, R. (1997) Endocr. Rev. 18:157.
  4. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
  5. Leung, D.W. et al. (1987) Nature 330:537.
  6. Stallings-Mann, J.L. et al. (1996) Proc. Nat. Acad. Sci. 93:12394.
  7. Amit, T. et al. (1997) Endocr. Metab. 82:3813.
  8. Ross, R.J.M., et al. (1997) Molecular Endocrinology 11:265.
  9. Carter-Su, C. et al. (1996) Annu. Rev. Physiol. 58:187.
  10. Postel-Vinay, M.C. and J. Finidori (1995) Eur. J. Endocrinol. 133:654.
Long Name
Growth Hormone Receptor
Entrez Gene IDs
2690 (Human); 14600 (Mouse); 25235 (Rat)
Alternate Names
GH receptor; GHBP; GHR; growth hormone binding protein; Growth Hormone R; growth hormone receptor; serum binding protein; Somatotropin receptor

Citations for Recombinant Human Growth Hormone R (GHR) Fc Chimera, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Structure and function of a dual antagonist of the human growth hormone and prolactin receptors with site-specific PEG conjugates
    Authors: Basu, R;Brody, R;Sandbhor, U;Kulkarni, P;Davis, E;Swegan, D;Caggiano, LJ;Brenya, E;Neggers, S;Kopchick, JJ;
    The Journal of biological chemistry
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  2. Highly efficient ribosome display selection by use of purified components for in vitro translation.
    Authors: Villemagne D, Jackson R, Douthwaite JA
    J. Immunol. Methods, 2006-05-11;313(1):140-8.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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Reviews for Recombinant Human Growth Hormone R (GHR) Fc Chimera, CF

Average Rating: 4.5 (Based on 2 Reviews)

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Recombinant Human Growth Hormone R (GHR) Fc Chimera, CF
By Anonymous on 05/19/2016
Application: Binding assay/Protein-protein interaction
Reason for Rating: Works consistently well for our binding assay.

Recombinant Human Growth Hormone R (GHR) Fc Chimera, CF
By Anonymous on 04/26/2016
Application: Binding assay/Protein-protein interaction