Recombinant Human Growth Hormone R (GHR) Fc Chimera, CF

R&D Systems | Catalog # 1210-GR

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Human Growth Hormone R (GHR) Fc Chimera (1210-GR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P10912

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all Growth Hormone R/GHR Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Growth Hormone R/GHR protein
Human GHR
(Ala27-Tyr264)
Accession # P10912		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala27

Predicted Molecular Mass

54 kDa (monomer)

SDS-PAGE

75-90 kDa, reducing conditions

Activity

Measured by its ability to inhibit GH-induced proliferation of Nb2‑11 rat lymphoma cells. Gout, P.W. et al. (1980) Cancer Res. 40:2433.
The ED50 for this effect is 0.4-2.0 ng/mL in the presence of 0.2 ng/mL of rhGH.

Reviewed Applications

Read 2 reviews rated 4.5 using 1210-GR in the following applications:

Formulation, Preparation, and Storage

1210-GR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Growth Hormone R/GHR

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I, to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Human GHR cDNA encodes a 638 amino acid (aa) residue type I transmembrane protein with an 18 aa putative signal peptide, a 246 aa extracellular domain, a 24 aa transmembrane domain and a 350 aa cytoplasmic domain (5). At least two alternatively spliced isoforms of human GHR, lacking the sequence encoded by exon 3, or lacking most of the cytoplasmic domain, also exist (6, 7). Soluble GH-binding proteins corresponding to extracellular domain of the transmembrane proteins can be generated from the membrane proteins (8). Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (9). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (8, 10).

References

  1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
  2. Le Roith, D. et al. (2001) Endocrine Rev. 22:53.
  3. Clark, R. (1997) Endocr. Rev. 18:157.
  4. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
  5. Leung, D.W. et al. (1987) Nature 330:537.
  6. Stallings-Mann, J.L. et al. (1996) Proc. Nat. Acad. Sci. 93:12394.
  7. Amit, T. et al. (1997) Endocr. Metab. 82:3813.
  8. Ross, R.J.M., et al. (1997) Molecular Endocrinology 11:265.
  9. Carter-Su, C. et al. (1996) Annu. Rev. Physiol. 58:187.
  10. Postel-Vinay, M.C. and J. Finidori (1995) Eur. J. Endocrinol. 133:654.

Long Name

Growth Hormone Receptor

Alternate Names

GHR

Entrez Gene IDs

2690 (Human); 14600 (Mouse); 25235 (Rat)

Gene Symbol

GHR

UniProt

P10912

Additional Growth Hormone R/GHR Products

Product Documents for Recombinant Human Growth Hormone R (GHR) Fc Chimera, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Growth Hormone R (GHR) Fc Chimera, CF

For research use only

Citations for Recombinant Human Growth Hormone R (GHR) Fc Chimera, CF

Customer Reviews for Recombinant Human Growth Hormone R (GHR) Fc Chimera, CF (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Recombinant Human Growth Hormone R (GHR) Fc Chimera, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Recombinant Human Growth Hormone R (GHR) Fc Chimera, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 05/19/2016
  • Recombinant Human Growth Hormone R (GHR) Fc Chimera, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 04/26/2016

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes