Cellular inhibitor of apoptosis protein 1 (cIAP-1, also known as BIRC2, MIHB, and HIAP2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. Structurally, cIAP-1 is comprised of 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). Functionally, cIAP-1 inhibit caspases through the direct interaction of its BIR domain with the active caspase. The ring finger domain of cIAP-1 also functions as an E3 ubiquitin ligase to ubiquitinate specific target proteins. Caspase activity may be restored by mitochondrial proteins, such as SMAC/Diablo or HtrA2/Omi, through interactions with the Reaper-like motif and the BIR domain.
Recombinant Human His10-cIAP-1/HIAP-2 Protein, CF
R&D Systems | Catalog # E3-280
Key Product Details
Source
Accession Number
Applications
Product Specifications
Source
Contains an N-terminal 10-His tag
Purity
Predicted Molecular Mass
Activity
Recombinant Human cIAP-1/HIAP-2 is a RING finger Ubiquitin ligase (E3) that functions downstream of a Ubiquitinactivating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial cIAP-1 concentration of 0.2-1 μM.
Formulation, Preparation, and Storage
E3-280
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 500 mM NaCl, 20% Glycerol (v/v), 5 mM TCEP, 0.5 mM EDTA |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: cIAP-1/HIAP-2
References
- Gyrd-Hansen M. & Meier P. (2010) Nat. Rev. Cancer 10: 561
- Bertrand M.J, et al. (2011) PLoS ONE 6: E22356
- Kulathila R., et al. (2009) Acta Crystallogr. D 65: 58
- Lopez J., et al. (2011) Mol. Cell 42: 569
Long Name
Alternate Names
Gene Symbol
UniProt
Additional cIAP-1/HIAP-2 Products
Product Documents for Recombinant Human His10-cIAP-1/HIAP-2 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human His10-cIAP-1/HIAP-2 Protein, CF
For research use only
Related Research Areas
Customer Reviews for Recombinant Human His10-cIAP-1/HIAP-2 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human His10-cIAP-1/HIAP-2 Protein, CF and earn rewards!
Have you used Recombinant Human His10-cIAP-1/HIAP-2 Protein, CF?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Associated Pathways
NOD-like Receptor Signaling Pathways
Ubiquitination Cascade Pathway
