Recombinant Human HS2ST1 Protein, CF

Catalog # Availability Size / Price Qty
6335-ST-020
Product Details
Citations (1)
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Recombinant Human HS2ST1 Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to transfer sulfate from PAPS to heparan sulfate. The specific activity is >75 pmol/min/μg, as measured under the described conditions.
Source
Chinese Hamster Ovary cell line, CHO-derived human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 protein
Met59-Asn356, with an N-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
36 kDa
SDS-PAGE
38-40 kDa, reducing conditions

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Background: Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1

Heparan sulfate is a highly sulfated polysaccharide found on the cell surface and within the extracellular matrix. Typically, it is covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues are either glucuronic acid or iduronic acid and maybe sulfated at the 2-O position by heparan sulfate 2‑O sulfotransferase 1 (HS2ST1) (3, 4). HS2ST1 physically interacts in the Golgi apparatus with glucuronyl c5-epimerase (5), which catalyzes the conversion of glucuronic acid to iduronic acid (6). As a consequence, 2-O sulfation predominantly occurs on iduronic acids naturally and overexpression of HS2ST1 alone causes an increase in 2-O sulfation on glucuronic acid (7). The enzyme activity of the recombinant human HS2ST1 was measured using a PAP-specific phosphatase-coupled sulfotransferase assay (8).

References
  1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
  2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
  3. Kobayashi, M. et al. (1996) J. Biol. Chem. 271:7645.
  4. Bullock, S.L. et al. (1998) Genes & Development 12:1894.
  5. Li, J.P. et al. (2001) J. Biol. Chem. 276:20069.
  6. Pinhal, M.A.S et al. (2001) Proc. Natl. Acad. Sci. USA. 98:12984.
  7. Rong, J. et al. (2000) Biochem. J. 346:463.
  8. Prather, B. et al. (2012) Anal Biochem. 423:86.
Entrez Gene IDs
9653 (Human)
Alternate Names
2OST; 2-O-sulfotransferase; EC 2.8.2; EC 2.8.2.-; FLJ11317; heparan sulfate 2-O-sulfotransferase 1; HS2ST; HS2ST1; KIAA0448dJ604K5.2; MGC131986

Citation for Recombinant Human HS2ST1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Structural and functional study of D-glucuronyl C5-epimerase.
    Authors: Qin Y, Ke J, Gu X, Fang J, Wang W, Cong Q, Li J, Tan J, Brunzelle J, Zhang C, Jiang Y, Melcher K, Li J, Xu H, Ding K
    J Biol Chem, 2015;290(8):4620-30.
    Species: Zebrafish
    Sample Types: Protein
    Applications: Bioassay

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