Universal Sulfotransferase Activity Kit

Measurement of sulfotransferase activity
Catalog # Availability Size / Price Qty
EA003
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Universal Sulfotransferase Activity Kit Summary

View Full Assay Principle

The Universal Sulfotransferase Activity Kit (Catalog # EA003) provides a simple, non-radioactive high-throughput compatible format for assaying the enzyme activity of all sulfotransferases that use 3-phosphoadenosine-5-phosphosulfate (PAPS) as the donor substrate. This kit utilizes inositol monophosphatase 3 (IMPAD1) as a coupling phosphatase to remove inorganic phosphate quantitatively from the leaving nucleotide 3-phosphoadenosine-5-phosphate (PAP) that is generated during sulfotransferase reactions.

Features

  • Applicable for all sulfotransferases that use PAPS as the donor substrate
  • Non-radioactive
  • No time-consuming separation steps (e.g. column chromatography)
  • Provides accurate kinetic analysis of sulfotransferase reactions
  • Amenable to high-throughput analysis

Kit Contents

  • CHO cell-expressed Recombinant Mouse IMPAD1
  • PAP
  • Phosphatase Buffer 3
  • Phosphate Standard
  • Malachite Green Reagents A & B

Data Example

Activity of CHST7

Activity of CHST7
View Larger Image

The ability of Recombinant Mouse Carbohydrate Sulfotransferase 7/CHST7 (Catalog # 5108-ST) to transfer sulfate from PAPS to N-acetyl glucosamine was measured using the Universal Sulfotransferase Activity Kit (Catalog # EA003). The corrected optical densities were plotted versus the amount of enzyme. Specific activity of CHST7 was calculated to be 116.4 pmol/min/μg.

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-Species

Product Datasheets

Reagents Provided

Other Reagents Provided

Citations for Universal Sulfotransferase Activity Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Correlating chemical sensitivity and basal gene expression reveals mechanism of action.
    Authors: Rees M, Seashore-Ludlow B, Cheah J, Adams D, Price E, Gill S, Javaid S, Coletti M, Jones V, Bodycombe N, Soule C, Alexander B, Li A, Montgomery P, Kotz J, Hon C, Munoz B, Liefeld T, Dancik V, Haber D, Clish C, Bittker J, Palmer M, Wagner B, Clemons P, Shamji A, Schreiber S
    Nat Chem Biol, 2016;12(2):109-16.  2016
  2. Detection of specific glycosaminoglycans and glycan epitopes by in vitro sulfation using recombinant sulfotransferases.
    Authors: Wu ZL, Prather B, Ethen CM
    Glycobiology, 2011;21(5):625-33.  2011

FAQs

No product specific FAQs exist for this product, however you may

View all Activity Assay FAQs

Phosphatase Activity Assays

Recombinant Enzymes

Sulfotransferase Assays and Substrates

Supplemental ELISA Products

Reviews for Universal Sulfotransferase Activity Kit

There are currently no reviews for this product. Be the first to review Universal Sulfotransferase Activity Kit and earn rewards!

Have you used Universal Sulfotransferase Activity Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review