Universal Sulfotransferase Activity Kit
Universal Sulfotransferase Activity Kit Summary
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The Universal Sulfotransferase Activity Kit (Catalog # EA003) provides a simple, non-radioactive high-throughput compatible format for assaying the enzyme activity of all sulfotransferases that use 3-phosphoadenosine-5-phosphosulfate (PAPS) as the donor substrate. This kit utilizes inositol monophosphatase 3 (IMPAD1) as a coupling phosphatase to remove inorganic phosphate quantitatively from the leaving nucleotide 3-phosphoadenosine-5-phosphate (PAP) that is generated during sulfotransferase reactions.
- Applicable for all sulfotransferases that use PAPS as the donor substrate
- No time-consuming separation steps (e.g. column chromatography)
- Provides accurate kinetic analysis of sulfotransferase reactions
- Amenable to high-throughput analysis
- CHO cell-expressed Recombinant Mouse IMPAD1
- Phosphatase Buffer 3
- Phosphate Standard
- Malachite Green Reagents A & B
Activity of CHST7
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The ability of Recombinant Mouse Carbohydrate Sulfotransferase 7/CHST7 (Catalog # 5108-ST) to transfer sulfate from PAPS to N-acetyl glucosamine was measured using the Universal Sulfotransferase Activity Kit (Catalog # EA003). The corrected optical densities were plotted versus the amount of enzyme. Specific activity of CHST7 was calculated to be 116.4 pmol/min/μg.
Citations for Universal Sulfotransferase Activity Kit
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Correlating chemical sensitivity and basal gene expression reveals mechanism of action.
Authors: Rees M, Seashore-Ludlow B, Cheah J, Adams D, Price E, Gill S, Javaid S, Coletti M, Jones V, Bodycombe N, Soule C, Alexander B, Li A, Montgomery P, Kotz J, Hon C, Munoz B, Liefeld T, Dancik V, Haber D, Clish C, Bittker J, Palmer M, Wagner B, Clemons P, Shamji A, Schreiber S
Nat Chem Biol, 2016;12(2):109-16. 2016
Detection of specific glycosaminoglycans and glycan epitopes by in vitro sulfation using recombinant sulfotransferases.
Authors: Wu ZL, Prather B, Ethen CM
Glycobiology, 2011;21(5):625-33. 2011
Does the Malachite Green Phosphate Detection Kit (DY996) detect both pyrophosphate (diphosphate) and free phosphate (monophosphate)?
The Malachite Green Phosphate Detection Kit measures free inorganic phosphate in aqueous solutions. The assay principle is based on complex formation between malachite green molybdate and free phosphate under acidic conditions. Lipid bound, protein-bound, pyrophashate and other condensed phosphates must be hydrolyzed prior to measurement in the malachite green assay.
Phosphatase Activity Assays
Sulfotransferase Assays and Substrates
Supplemental ELISA Products
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