Recombinant Human ICOS Fc Chimera Protein, CF

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Recombinant Human ICOS Fc Chimera Protein Bioactivity
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Product Details
Citations (3)
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Reviews (6)

Recombinant Human ICOS Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit human T cell proliferation induced by rhB7-H2/Fc Chimera in the presence of anti-CD3. Wang, S. et al. (2000) Blood 96:2808. The ED50 for this effect is 1-4 µg/mL in the presence of 3 µg/mL Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) and at least 20 ng/mL of anti-CD3.
Optimal dilutions should be determined by each laboratory for each application.
Mouse myeloma cell line, NS0-derived human ICOS protein
Human ICOS
Accession # Q9Y6W8
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40.3 kDa (monomer)
50-60 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human ICOS Fc Chimera Protein Bioactivity View Larger

Recombinant Human ICOS Fc Chimera (Catalog # 169-CS) inhibits rhB7-H2/Fc Chimera (Catalog # 165-B7) induced human T cell proliferation in the presence of anti-CD3. The ED50 for this effect is 1-4 µg/mL.

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Background: ICOS

Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24 - 48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

  1. Aicher, A. et al. (2000) J. Immunol. 164:4689.
  2. Coyle, A.J. et al. (2000) Immunity 13:95.
  3. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
  5. Hutloff, A. et al. (1999) Nature 397:263.
  6. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  7. Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name
Inducible Co-stimulator
Entrez Gene IDs
29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)
Alternate Names
Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; ICOS; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850

Citations for Recombinant Human ICOS Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Orthogonal quantification of soluble inducible T-cell costimulator (ICOS) in healthy and diseased human serum
    Authors: K McKinski, D McNulty, F Zappacosta, M Birchler, M Szapacs, C Evans
    Journal of pharmaceutical analysis, 2022;12(2):317-323.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA Standard
  2. Nonimmune cell-derived ICOS ligand functions as a renoprotective alphavbeta3 integrin-selective antagonist
    Authors: KH Koh, Y Cao, S Mangos, NJ Tardi, RR Dande, HW Lee, B Samelko, MM Altintas, VP Schmitz, H Lee, K Mukherjee, V Peev, DJ Cimbaluk, J Reiser, E Hahm
    J. Clin. Invest., 2019;129(4):1713-1726.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  3. ICOS cooperates with CD28, IL-2, and IFN-gamma and modulates activation of human naive CD4+ T cells.
    Authors: Mesturini R, Nicola S, Chiocchetti A, Bernardone IS, Castelli L, Bensi T, Ferretti M, Comi C, Dong C, Rojo JM, Yagi J, Dianzani U
    Eur. J. Immunol., 2006;36(10):2601-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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Reviews for Recombinant Human ICOS Fc Chimera Protein, CF

Average Rating: 4.8 (Based on 6 Reviews)

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Recombinant Human ICOS Fc Chimera Protein, CF
By Sarah Garrett on 11/15/2019
Application: Binding assay/Protein-protein interaction

Protein A beads were precoated with hICOS-L protein and incubated with increasing concentration of hIgG1 control or ICOS hIgG1 to a final concentration of 0 to 500nM. Binding was monitored by flow cytometry using anti-human 488 secondary antibody for detection.

Recombinant Human ICOS Fc Chimera Protein, CF
By Travis Durkin on 07/08/2019
Application: Characterization prior to use
Reason for Rating: 83&amp; homogeneous

Recombinant Human ICOS Fc Chimera Protein, CF
By Jenna Nguyen on 03/25/2019
Application: Media additive for protein or antibody production

Recombinant Human ICOS Fc Chimera Protein, CF
By Jenna Nguyen on 10/24/2018
Application: SDS-PAGE Control

Recombinant Human ICOS Fc Chimera Protein, CF
By Jenna Nguyen on 10/12/2018
Application: Binding assay/Protein-protein interaction

Recombinant Human ICOS Fc Chimera Protein, CF
By Jenna Nguyen on 07/11/2018
Application: Apoptosis assay