Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24 - 48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
Recombinant Human ICOS Fc Chimera Protein, CF
R&D Systems | Catalog # 169-CS
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Key Product Details
- R&D Systems NS0-derived Recombinant Human ICOS Fc Chimera Protein (169-CS)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Structure / Form
Applications
Product Specifications
Source
| Human ICOS (Glu21-Phe141) Accession # Q9Y6W8 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Measured by its ability to inhibit human T cell proliferation induced by rhB7-H2/Fc Chimera in the presence of anti-CD3. Wang, S. et al. (2000) Blood 96:2808.
The ED50 for this effect is 1-4 µg/mL in the presence of 3 µg/mL Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) and at least 20 ng/mL of anti-CD3.
Optimal dilutions should be determined by each laboratory for each application.
Reviewed Applications
Read 7 reviews rated 4.7 using 169-CS in the following applications:
Scientific Data Images for Recombinant Human ICOS Fc Chimera Protein, CF
Recombinant Human ICOS Fc Chimera Protein Bioactivity
Recombinant Human ICOS Fc Chimera (Catalog # 169-CS) inhibits rhB7-H2/Fc Chimera (Catalog # 165-B7) induced human T cell proliferation in the presence of anti-CD3. The ED50 for this effect is 1-4 µg/mL.
Formulation, Preparation, and Storage
169-CS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: ICOS
References
- Aicher, A. et al. (2000) J. Immunol. 164:4689.
- Coyle, A.J. et al. (2000) Immunity 13:95.
- Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
- Hutloff, A. et al. (1999) Nature 397:263.
- Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional ICOS Products
Product Documents for Recombinant Human ICOS Fc Chimera Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human ICOS Fc Chimera Protein, CF
For research use only
Citations for Recombinant Human ICOS Fc Chimera Protein, CF
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Customer Reviews for Recombinant Human ICOS Fc Chimera Protein, CF (7)
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Customer Images
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 07/13/2024
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 11/15/2019Protein A beads were precoated with hICOS-L protein and incubated with increasing concentration of hIgG1 control or ICOS hIgG1 to a final concentration of 0 to 500nM. Binding was monitored by flow cytometry using anti-human 488 secondary antibody for detection.
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Application: Characterization prior to useVerified Customer | Posted 07/08/2019
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Application: Media additive for protein or antibody productionVerified Customer | Posted 03/25/2019
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Application: SDS-PAGE ControlVerified Customer | Posted 10/24/2018
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 10/12/2018
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Application: Apoptosis assayVerified Customer | Posted 07/11/2018
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