Recombinant Human IFN-alpha-2B Protein, CF

Catalog # Availability Size / Price Qty
11013-IF-010
11013-IF-050
Recombinant Human IFN-alpha-2B Protein Bioactivity.
2 Images
Product Details
Citations (2)
FAQs
Reviews

Recombinant Human IFN-alpha-2B Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 fot this effect is 3.00-60.0 pg/mL.
Source
Human embryonic kidney cell, HEK293-derived human IFN-alpha protein
Cys24-Glu188
Accession #
N-terminal Sequence
Analysis
Cys24
Predicted Molecular Mass
19 kDa
SDS-PAGE
18-22 kDa, under reducing conditions.

Product Datasheets

You must select a language.

x

11013-IF

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11013-IF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Recombinant Human IFN-alpha-2B Protein demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 3.00-60.0 pg/mL

SDS-PAGE View Larger

2 μg/lane of Recombinant Human IFN-alpha-2B Protein (Catalog # 11013-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-22 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IFN-alpha

Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). There are more than a dozen closely related IFN alpha subtypes found in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2, 3). There are several variants of human IFNA2, IFNA2a, IFNA2b and IFNA2c, which only differ by a few point mutations (4). The extracellular domain (ECD) of mature human IFNA2b shares 59% aa sequence identity with mouse IFNA2. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (5, 6). While individual IFN alpha subtypes are known to display unique efficacies to viral protection, nothing is known about functions unique to IFNA2 in human physiology. However, IFNA2 has become the representative type I IFN subtype used in basic research and numerous clinical applications (7). Since being first approved for treatment of hairy cell leukemia in 1986, IFNA2b has been approved for the treatment of several malignancies including AIDS-related Kaposi's sarcoma, malignant melanoma and chronic hepatitis B and C (8). More recently, human IFNA2b has been tested as a potential treatment for COVID-19 (9).

References
  1. Pestka S. et al. (1987) Annu Rev Biochem. 56:727.
  2. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  3. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  4. Lee, N. et al. (1995) J. Interferon Cytokine Res. 15:341.
  5. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  6. James, C.M. et al. (2007) Vaccine. 25(10):1856.
  7. Paul, F. et al. (2015) Gene. 567:132.
  8. Kiladjian, J.J. et al. (2008) Leukemia 22:1990.
  9. Zhou, Q. et al. (2020) Front Immunol. 11:1061.
Long Name
Interferon alpha
Entrez Gene IDs
3440 (Human)
Alternate Names
IFNA; IFNA2; IFNA2b; IFNalpha; IFN-alpha; IFN-alphaA; INFA2; interferon alpha 2; LeIF D

Citations for Recombinant Human IFN-alpha-2B Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Data supporting the roles of BAP1, STING, and IFN-beta in ISGF3 activation in ccRCC
    Authors: LE Langbein, E Sementino, Z Zhong, W Jiang, L Li, JR Testa, H Yang
    Data in Brief, 2022-11-11;45(0):108743.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. MicroRNA Profiles in Monocyte-Derived Macrophages Generated by Interleukin-27 and Human Serum: Identification of a Novel HIV-Inhibiting and Autophagy-Inducing MicroRNA
    Authors: T Imamichi, S Goswami, X Hu, S Laverdure, J Yang, J Qiu, Q Chen, BT Sherman, W Chang
    International Journal of Molecular Sciences, 2021-01-28;22(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Reference Standard

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human IFN-alpha-2B Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human IFN-alpha-2B Protein, CF and earn rewards!

Have you used Recombinant Human IFN-alpha-2B Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review