Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF New

Catalog #: 11655-GR Datasheet / COA / SDS

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11655-GR-050

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Recombinant Human IGF-I R/IGF1R Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF Summary

R&D Systems HEK293-derived Recombinant Human IGF-I R/IGF1R Fc Chimera Protein (11655-GR)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
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Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human IGF-I R/IGF1R Fc Chimera (Catalog # 11655-GR binds to Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) with an ED₅₀ of 3.00-45.0 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human IGF-I R/IGF1R protein

Human IGF1R (Glu31-His935) Accession # P08069.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

N-terminal Sequence
Analysis

Glu31 (apha subunit), Asp741 (beta subunit)

Structure / Form

Disulfide linked homodimer

Predicted Molecular Mass

130 kDa (single chain), 80 kDa (alpha subunit) and 49 kDa (beta subunit)

SDS-PAGE

160-180 kDa, 110-125 kDa and 70-80 kDa, under reducing conditions

Product Datasheets

11655-GR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11655-GR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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In a functional ELISA, Recombinant Human IGF-I R/IGF1R Fc Chimera Protein (Catalog # 11655-GR) binds to Recombinant Human IGF-I/IGF-1 (291-G1) with an ED₅₀ of 3.00-45.0 ng/mL.

SDS-PAGE

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2 μg/lane of (Catalog # 11655-GR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 160-180 kDa, 110-125 kDa and 70-80 kDa, under reducing conditions.

Reconstitution Calculator

Background: IGF-I R/IGF1R

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

References

Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.

Long Name

Insulin-like Growth Factor I Receptor

Entrez Gene IDs

3480 (Human)

Alternate Names

CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2