Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF

R&D Systems | Catalog # 305-GR

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems NS0-derived Recombinant Human IGF-I R/IGF1R (His-tagged) Protein (305-GR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P08069

Applications

Binding Activity
View all IGF-I R/IGF1R Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IGF-I R/IGF1R protein
Met1-Asn932 with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu31 and Asp741

Predicted Molecular Mass

104 kDa (single chain), 81 kDa ( alpha subunit) and 23 kDa ( beta subunit)

SDS-PAGE

150 kDa, 120 kDa and 48 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IGF‑I R/IGF1R (Catalog # 305-GR) binds to Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) with an ED50 of 2.50-30.0 ng/mL.

Scientific Data Images for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF

Recombinant Human IGF-I R/IGF1R (Histidine-tagged) Protein SEC-MALS.

Recombinant Human IGF1R/C-His Protein (Catalog # 305-GR) has a molecular weight (MW) of 296.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a heterotetramer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Formulation, Preparation, and Storage

305-GR
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl, EDTA and Tween®-20 with Trehalose.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGF-I R/IGF1R

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta  subunits. Both the alpha  and beta  subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta  subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

References

  1. Rechler, M.M. and S.P. Nissley (1990) Insulin-Like Growth Factors, in Peptide Growth Factors and Their Receptors I, M.B. Sporn and A.B. Roberts, editors, Springer-Verlag, New York, New York, p. 263.

Long Name

Insulin-like Growth Factor I Receptor

Alternate Names

CD221, IGF-1R, IGF-IR, IGF1R, IGFIR

Entrez Gene IDs

3480 (Human)

Gene Symbol

IGF1R

UniProt

P08069

Additional IGF-I R/IGF1R Products

Product Documents for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF

For research use only

Citations for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF

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