Recombinant Human IL-1 alpha/IL-1F1 Protein 200-LA-002: R&D Systems

Recombinant Human IL-1 alpha/IL-1F1 Protein

(37 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 1‑6 pg/mL.
  • Source
    E. coli-derived Ser113-Ala271
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser113
  • Predicted Molecular Mass
    18 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
200-LA
 
200-LA/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Data Images
Recombinant Human IL-1 alpha /IL-1F1 (Catalog # 200-LA) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 1-6 pg/mL.

1 μg/lane of Recombinant Human
IL-1 alpha /IL-1F1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.

Background: IL-1 alpha/IL-1F1

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

  • Long Name:
    Interleukin 1 alpha
  • Entrez Gene IDs:
    3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
  • Alternate Names:
    Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Interleukin-17A-induced production of acute serum amyloid A by keratinocytes contributes to psoriasis pathogenesis
    Authors: E Couderc, F Morel, P Levillain, A Buffière-M, M Camus, C Paquier, C Bodet, JF Jégou, M Pohin, L Favot, M Garcia, V Huguier, J Mcheik, C Lacombe, H Yssel, G Guillet, FX Bernard, JC Lecron
    PLoS ONE, 2017;12(7):e0181486.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Atorvastatin Promotes Phagocytosis and Attenuates Pro-Inflammatory Response in Human Retinal Pigment Epithelial Cells
    Authors: B Tian, A Al-Moujahe, P Bouzika, Y Hu, S Notomi, P Tsoka, JW Miller, H Lin, DG Vavvas
    Sci Rep, 2017;7(1):2329.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Interleukin-1 primes human mesenchymal stem cells towards an anti-inflammatory and pro-trophic phenotype in vitro
    Authors: E Redondo-Ca, C Cunningham, J Miller, L Martuscell, S Aoulad-Ali, NJ Rothwell, CM Kielty, SM Allan, E Pinteaux
    Stem Cell Res Ther, 2017;8(1):79.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Prevalence and correlation of cytokine-specific autoantibodies with epidemiological factors and C-reactive protein in 8,972 healthy individuals: Results from the Danish Blood Donor Study
    Authors: JH von Steman, AS Rigas, LW Thørner, DGK Rasmussen, OB Pedersen, K Rostgaard, C Erikstrup, H Ullum, MB Hansen
    PLoS ONE, 2017;12(6):e0179981.
    Species: Human
    Sample Type: Plasma
    Application: Ctrl
  5. Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients
    PLoS ONE, 2016;11(9):e0162908.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Characterization of the MMP/TIMP Imbalance and Collagen Production Induced by IL-1? or TNF-? Release from Human Hepatic Stellate Cells
    Authors: S Robert, T Gicquel, A Bodin, V Lagente, E Boichot
    PLoS ONE, 2016;11(4):e0153118.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Smooth Muscle Enriched Long Non-Coding RNA (SMILR) Regulates Cell Proliferation
    Authors: MD Ballantyne, K Pinel, R Dakin, AT Vesey, L Diver, R Mackenzie, R Garcia, P Welsh, N Sattar, G Hamilton, N Joshi, MR Dweck, JM Miano, MW McBride, DE Newby, RA McDonald, AH Baker
    Circulation, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Myostatin is a direct regulator of osteoclast differentiation and its inhibition reduces inflammatory joint destruction in mice.
    Authors: Dankbar B, Fennen M, Brunert D, Hayer S, Frank S, Wehmeyer C, Beckmann D, Paruzel P, Bertrand J, Redlich K, Koers-Wunrau C, Stratis A, Korb-Pap A, Pap T
    Nat Med, 2015;21(9):1085-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Brief Glutamine Pretreatment Increases Alveolar Macrophage CD163/Heme Oxygenase-1/p38-MAPK Dephosphorylation Pathway and Decreases Capillary Damage but Not Neutrophil Recruitment in IL-1/LPS-Insufflated Rats.
    Authors: Fernandez-Bustamante A, Agazio A, Wilson P, Elkins N, Domaleski L, He Q, Baer K, Moss A, Wischmeyer P, Repine J
    PLoS ONE, 2015;10(7):e0130764.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  10. MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation.
    Authors: Laberge R, Sun Y, Orjalo A, Patil C, Freund A, Zhou L, Curran S, Davalos A, Wilson-Edell K, Liu S, Limbad C, Demaria M, Li P, Hubbard G, Ikeno Y, Javors M, Desprez P, Benz C, Kapahi P, Nelson P, Campisi J
    Nat Cell Biol, 2015;17(8):1049-61.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1alpha, TNFalpha and oncostatin M.
    Authors: Rabeony H, Petit-Paris I, Garnier J, Barrault C, Pedretti N, Guilloteau K, Jegou J, Guillet G, Huguier V, Lecron J, Bernard F, Morel F
    PLoS ONE, 2014;9(7):e101937.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Fetal human keratinocytes produce large amounts of antimicrobial peptides: involvement of histone-methylation processes.
    Authors: Gschwandtner M, Zhong S, Tschachler A, Mlitz V, Karner S, Elbe-Burger A, Mildner M
    J Invest Dermatol, 2014;134(8):2192-201.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33.
    J. Exp. Med., 2012;209(8):1505-17.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Evidence for altered Wnt signaling in psoriatic skin.
    Authors: Gudjonsson JE, Johnston A, Stoll SW
    J. Invest. Dermatol., 2010;130(7):1849-59.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
    Authors: Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
    Int. J. Cancer, 2010;127(2):332-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis.
    Authors: Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, Guillet G, Bernard FX, Lecron JC, Morel F
    J. Immunol., 2010;184(0):5263-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  19. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  20. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007;53(11):1990-5.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  21. Bradykinin potentiates cytokine-induced prostaglandin biosynthesis in osteoblasts by enhanced expression of cyclooxygenase 2, resulting in increased RANKL expression.
    Authors: Brechter AB, Lerner UH
    Arthritis Rheum., 2007;56(3):910-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  23. IL-10 inhibits endothelium-dependent T cell costimulation by up-regulation of ILT3/4 in human vascular endothelial cells.
    Authors: Gleissner CA, Zastrow A, Klingenberg R, Kluger MS, Konstandin M, Celik S, Haemmerling S, Shankar V, Giese T, Katus HA, Dengler TJ
    Eur. J. Immunol., 2007;37(1):177-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
    Authors: Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC
    Br. J. Pharmacol., 2007;151(1):115-27.
    Species: N/A
    Sample Type: N/A
    Application: WB Ctrl
  25. L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
    Authors: Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM
    Atherosclerosis, 2007;192(1):56-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  27. Synergism of TNF and IL-1 in the induction of matrix metalloproteinase-3 in trabecular meshwork.
    Authors: Kelley MJ, Rose AY, Song K, Chen Y, Bradley JM, Rookhuizen D, Acott TS
    Invest. Ophthalmol. Vis. Sci., 2007;48(6):2634-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  28. Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
    Authors: Li Y, Fan J, Chen M, Li W, Woodley DT
    J. Invest. Dermatol., 2006;126(9):2096-105.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  29. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
    Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
    J. Exp. Med., 2006;203(12):2763-77.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  30. Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1).
    Authors: Palmer G, Trolliet S, Talabot-Ayer D, Mezin F, Magne D, Gabay C
    Cytokine, 2005;31(2):153-60.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  31. CYP7B expression and activity in fibroblast-like synoviocytes from patients with rheumatoid arthritis: regulation by proinflammatory cytokines.
    Authors: Dulos J, van der Vleuten MA, Kavelaars A, Heijnen CJ, Boots AM
    Arthritis Rheum., 2005;52(3):770-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  32. Interleukin-1 inhibits voltage-dependent P/Q-type Ca2+ channel associated with the inhibition of the rise of intracellular free Ca2+ concentration and catecholamine release in adrenal chromaffin cells.
    Authors: Morita K, Miyasako T, Kitayama S, Dohi T
    Biochim. Biophys. Acta, 2004;1673(3):160-9.
    Species: Bovine
    Sample Type: Whole Cells
    Application: Bioassay
  33. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
    Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
    J. Immunol., 2004;173(5):3482-91.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  34. The effect of inflammatory cytokines on secretion of macrophage colony-stimulating factor and monocyte chemoattractant protein-1 in human granulosa cells.
    Authors: Kawano Y, Fukuda J, Itoh H, Takai N, Nasu K, Miyakawa I
    Am. J. Reprod. Immunol., 2004;52(2):124-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  35. The soluble form of IL-1 receptor accessory protein enhances the ability of soluble type II IL-1 receptor to inhibit IL-1 action.
    Authors: Smith DE, Hanna R, Della Friend, Moore H, Chen H, Farese AM, MacVittie TJ, Virca GD, Sims JE
    Immunity, 2003;18(1):87-96.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  36. c-Jun and JunB antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin.
    Authors: Szabowski A, Maas-Szabowski N, Andrecht S, Kolbus A, Schorpp-Kistner M, Fusenig NE, Angel P
    Cell, 2000;103(5):745-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  37. Gingival crevicular fluid from patients with periodontitis contains bone resorbing activity.
    Authors: Lerner UH, Modeer T, Krekmanova L, Claesson R, Rasmussen L
    Eur. J. Oral Sci., 1998;106(3):778-87.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 37 Citations

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