Recombinant Human IL-12/IL-23 p40 Monomer Protein Summary
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: IL-12/IL-23 p40
Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 is produced by macrophages and B lymphocytes and has multiple effects on T cells and NK cells, including stimulation of cytotoxic activity, proliferation, and promotion of Th1 development as well as IFN-gamma and TNF production. IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subunits by themselves have no IL-12 activity, the p40 dimer has been shown to bind the IL-12 receptor and to be an IL-12 antagonist. Free p35 has not been detected in supernatant solutions of cultured cells expressing only p35 or both p35 and p40 mRNAs. In contrast, p40 is secreted in excess of IL-12 in cells expressing both p35 and p40 mRNAs. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.
Citations for Recombinant Human IL-12/IL-23 p40 Monomer Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 6
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IL-12 p40 monomer is different from other IL-12 family members to selectively inhibit IL-12R&beta1 internalization and suppress EAE
Authors: S Mondal, M Kundu, M Jana, A Roy, SB Rangasamy, KK Modi, J Wallace, YA Albalawi, R Balabanov, K Pahan
Proc. Natl. Acad. Sci. U.S.A., 2020;117(35):21557-21567.
Sample Types: In Vivo
The localisation of the heparin binding sites of human and murine interleukin-12 within the carboxyterminal domain of the P40 subunit
Authors: P Garnier, R Mummery, MJ Forster, B Mulloy, RV Gibbs, CC Rider
Sample Types: Complex Sample Type
Free IL-12p40 monomer is a polyfunctional adaptor for generating novel IL-12-like heterodimers extracellularly.
Authors: Abdi K, Singh N, Spooner E, Kessler B, Radaev S, Lantz L, Xiao T, Matzinger P, Sun P, Ploegh H
J Immunol, 2014;192(12):6028-36.
Elevation of IL-12 p40 and its antibody in myasthenia gravis with thymoma.
Authors: Yoshikawa H, Sato K, Edahiro S, Furukawa Y, Maruta T, Iwasa K, Watanabe H, Takaoka S, Suzuki Y, Takamori M, Yamada M
J. Neuroimmunol., 2006;175(1):169-75.
Sample Types: Serum
Applications: ELISA (Capture)
Diesel exhaust exposure favors TH2 cell recruitment in nonatopic subjects by differentially regulating chemokine production.
Authors: Chang Y, Senechal S, de Nadai P, Chenivesse C, Gilet J, Vorng H, Legendre B, Tonnel AB, Wallaert B, Lassalle P, Tsicopoulos A
J. Allergy Clin. Immunol., 2006;118(2):354-60.
Sample Types: Whole Cells
IL-23 is increased in dendritic cells in multiple sclerosis and down-regulation of IL-23 by antisense oligos increases dendritic cell IL-10 production.
Authors: Vaknin-Dembinsky A, Balashov K, Weiner HL
J. Immunol., 2006;176(12):7768-74.
Sample Types: N/A
Applications: ELISA (Specificity Testing)
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