Recombinant Human IL-12 (linked heterodimer) Protein, CF

GS Linker, Defined heterodimer stoichiometry
Catalog # Availability Size / Price Qty
10018-IL-010
10018-IL-050
Recombinant Human IL-12 (linked heterodimer) Protein, CF Bioactivity
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Recombinant Human IL-12 (linked heterodimer) Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
The ED50 for this effect is 0.01-0.05 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human IL-12 protein
Human IL-12p40
(Ile23-Ser328)
Accession # P29460
GSGSSRGGSGSGGSGGGGSKHuman IL-12p35
(Arg23-Ser219)
Accession # P29459
N-terminusC-terminus
N-terminal Sequence
Analysis
Ile23
Structure / Form
GS-linked heterodimer
Predicted Molecular Mass
59 kDa
SDS-PAGE
63-75 kDa, reducing conditions

Product Datasheets

10018-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100-200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Human IL-12 (linked heterodimer) Protein, CF Bioactivity View Larger

Recombinant Human IL-12 (Catalog # 10018-IL) stimulatesproliferation in PHA-activated human T lymphoblasts. The ED50 for this effectis 0.01-0.05 ng/mL.

SDS-PAGE Recombinant Human IL-12 (linked heterodimer) Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Human IL‑12 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 63-75 kDa.

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Background: IL-12

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines (1). The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF (2, 3). These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells (4). These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN‑gamma inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells (5). Current evidence indicates that IL‑12, produced by macrophages in response to infectious agents, is a central mediator of the cell‑mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.

References
  1. Gubler, U. et al. (1991) Proc. Natl. Acad. Sci. 88:4143.
  2. Gearing, D. et al. (1991) Cell 66:9.
  3. Merberg, D. et al. (1992) Immunology Today 13:78.
  4. Wolf, S.F. et al. (1991) Journal of Immunology 146:3074.
  5. Airoldi, I. et al. (2000) Journal of Immunology 165:6880.
Long Name
Interleukin 12
Entrez Gene IDs
3592 (Human); 16159 (Mouse); 84405 (Rat); 403977 (Canine); 493741 (Feline)
Alternate Names
CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35

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