Recombinant Human IL-15 Protein

(89 citations)
(1 Review)
  
  • Purity
    >97%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.3-2.6 ng/mL.
    The specific activity of Recombinant Human IL-15 is approximately 4.5 x 105 U/μg, which is calibrated against recombinant human IL-15 WHO International Standard (NIBSC code: 95/554).
  • Source
    E. coli-derived Asn49-Ser162
  • Accession #
  • N-terminal Sequence
    Analysis
    Asn49
  • Predicted Molecular Mass
    13 kDa
  • SDS-PAGE
    10 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
247-ILB
 
247-ILB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human IL-15 (Catalog #
247-ILB) stimulates cell proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.3-2.6 ng/mL.
1 µg/lane of Recombinant Human IL-15 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a major band at 10 kDa.
Background: IL-15
Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases (1, 2). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternative splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (3). IL-15 binds with high affinity to IL-15 R alpha (4). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (5). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (6). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (7). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (6). In human and mouse, soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding and bind up nearly all the IL-15 in circulation (8-10). Soluble IL-15 R alpha functions as an inhibitor that limits IL-15 action (4, 9). Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells (11). IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells (12 - 16). An important component of these functions is the ability of
IL-15 to induce dendritic cell differentiation and inflammatory activation (11, 14). IL-15 exhibits anti-tumor activity independent of its actions on NK cells or CD8+ T cells (17). It also inhibits the deposition of lipid in adipocytes, and its circulating levels are decreased in obesity (18).
  • References:
    1. De Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
    2. Grabstein, K. et al. (1994) Science 264:965.
    3. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
    4. Giri, J.G. et al. (1995) EMBO J. 14:3654.
    5. Giri, J. et al. (1994) EMBO J. 13:2822.
    6. Dubois, S. et al. (2002) Immunity 17:537.
    7. Castillo, E.F. and K.S. Schluns (2012) Cytokine 59:479.
    8. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
    9. Mortier, E. et al. (2004) J. Immunol. 173:1681.
    10. Bergamaschi, C. et al. (2012) Blood 120:e1.
    11. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
    12. Mortier, E. et al. (2003) J. Exp. Med. 205:1213.
    13. Gordy, L.E. et al. (2011) J. Immunol. 187:6335.
    14. Harris, K.M. (2011) J. Leukoc. Biol. 90:727.
    15. Xia, J. et al. (2010) Clin. Immunol. 134:130.
    16. Schluns, K.S. et al. (2002) J. Immunol. 168:4827.
    17. Davies, E. et al. (2010) J. Leukoc. Biol. 88:529.
    18. Barra, N.G. et al. (2010) Obesity 18:1601.
  • Long Name:
    Interleukin 15
  • Entrez Gene IDs:
    3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
  • Alternate Names:
    IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

89 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Clinical and immunological responses after CD30-specific chimeric antigen receptor-redirected lymphocytes
    Authors: CA Ramos, B Ballard, H Zhang, O Dakhova, AP Gee, Z Mei, M Bilgi, MF Wu, H Liu, B Grilley, CM Bollard, BH Chang, CM Rooney, MK Brenner, HE Heslop, G Dotti, B Savoldo
    J. Clin. Invest., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Hydroxychloroquine inhibits CD154 expression in CD4(+) T lymphocytes of systemic lupus erythematosus through NFAT, but not STAT5, signaling
    Authors: SF Wu, CB Chang, JM Hsu, MC Lu, NS Lai, C Li, CH Tung
    Arthritis Res. Ther., 2017;19(1):183.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Efficient regeneration of Human V?24(+) invariant NKT cells and their anti-tumor activity in vivo
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. IL-15 enhances the anti-tumor activity of trastuzumab against breast cancer cells but causes fatal side effects in humanized tumor mice (HTM)
    Oncotarget, 2016;0(0):.
    Species: Mouse
    Sample Type:
    Application: In Vivo
  5. CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes
    Sci Rep, 2016;6(0):34310.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Identification of a novel human memory T-cell population with the characteristics of stem-like chemo-resistance
    Oncoimmunology, 2016;5(6):e1165376.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes
    Authors: RB Jones, S Mueller, R O'Connor, K Rimpel, DD Sloan, D Karel, HC Wong, EK Jeng, AS Thomas, JB Whitney, SY Lim, C Kovacs, E Benko, S Karandish, SH Huang, MJ Buzon, M Lichterfel, A Irrinki, JP Murry, A Tsai, H Yu, R Geleziunas, A Trocha, MA Ostrowski, Irvi
    PLoS Pathog, 2016;12(4):e1005545.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Human memory T cells with a naive phenotype accumulate with aging and respond to persistent viruses
    Authors: Vesna Pulko
    Nat Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. CD123-Engager T Cells as a Novel Immunotherapeutic for Acute Myeloid Leukemia
    Mol Ther, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. IL-15 promotes activation and expansion of CD8+ T cells in HIV-1 infection
    Authors: Souheil-Antoine Younes
    J Clin Invest, 2016;126(7):2745-2756.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Novel chemoimmunotherapeutic strategy for hepatocellular carcinoma based on a genome-wide association study
    Sci Rep, 2016;6(0):38407.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Interferon Alpha Induces Sustained Changes in NK Cell Responsiveness to Hepatitis B Viral Load Suppression In Vivo
    PLoS Pathog, 2016;12(8):e1005788.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Evaluating Human T-Cell Therapy of Cytomegalovirus Organ Disease in HLA-Transgenic Mice.
    Authors: Thomas S, Klobuch S, Podlech J, Plachter B, Hoffmann P, Renzaho A, Theobald M, Reddehase M, Herr W, Lemmermann N
    PLoS Pathog, 2015;11(7):e1005049.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Broadly-specific cytotoxic T cells targeting multiple HIV antigens are expanded from HIV+ patients: implications for immunotherapy.
    Authors: Lam, Sharon, Sung, Julia, Cruz, Conrad, Castillo-Caro, Paul, Ngo, Minhtran, Garrido, Carolina, Kuruc, Joann, Archin, Nancie, Rooney, Cliona, Margolis, David, Bollard, Catherin
    Mol Ther, 2015;23(2):387-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Survivin-specific T cell receptor targets tumor but not T cells.
    Authors: Arber C, Feng X, Abhyankar H, Romero E, Wu M, Heslop H, Barth P, Dotti G, Savoldo B
    J Clin Invest, 2015;125(1):157-68.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. The natural killer cell dysfunction of aged mice is due to the bone marrow stroma and is not restored by IL-15/IL-15Ralpha treatment.
    Authors: Nair S, Fang M, Sigal L
    Aging Cell, 2015;14(2):180-90.
    Species: Mouse
    Sample Type:
    Application: In Vivo
  17. IL-15 amplifies the pathogenic properties of CD4+CD28- T cells in multiple sclerosis.
    Authors: Broux B, Mizee M, Vanheusden M, van der Pol S, van Horssen J, Van Wijmeersch B, Somers V, de Vries H, Stinissen P, Hellings N
    J Immunol, 2015;194(5):2099-109.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. Ex vivo cytosolic delivery of functional macromolecules to immune cells.
    Authors: Sharei A, Trifonova R, Jhunjhunwala S, Hartoularos G, Eyerman A, Lytton-Jean A, Angin M, Sharma S, Poceviciute R, Mao S, Heimann M, Liu S, Talkar T, Khan O, Addo M, von Andrian U, Anderson D, Langer R, Lieberman J, Jensen K
    PLoS ONE, 2015;10(4):e0118803.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. IRF-1 promotes liver transplant ischemia/reperfusion injury via hepatocyte IL-15/IL-15Ralpha production.
    Authors: Yokota S, Yoshida O, Dou L, Spadaro A, Isse K, Ross M, Stolz D, Kimura S, Du Q, Demetris A, Thomson A, Geller D
    J Immunol, 2015;194(12):6045-56.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  20. TLR-9 and IL-15 Synergy Promotes the In Vitro Clonal Expansion of Chronic Lymphocytic Leukemia B Cells.
    Authors: Mongini P, Gupta R, Boyle E, Nieto J, Lee H, Stein J, Bandovic J, Stankovic T, Barrientos J, Kolitz J, Allen S, Rai K, Chu C, Chiorazzi N
    J Immunol, 2015;195(3):901-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  21. Effect of Antihelminthic Treatment on Vaccine Immunogenicity to a Seasonal Influenza Vaccine in Primary School Children in Gabon: A Randomized Placebo-Controlled Trial.
    Authors: Bruckner S, Agnandji S, Berberich S, Bache E, Fernandes J, Schweiger B, Massinga Loembe M, Engleitner T, Lell B, Mordmuller B, Adegnika A, Yazdanbakhsh M, Kremsner P, Esen M
    PLoS Negl Trop Dis, 2015;9(6):e0003768.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Mature cytotoxic CD56(bright)/CD16(+) natural killer cells can infiltrate lymph nodes adjacent to metastatic melanoma.
    Cancer Res, 2015;74(0):81.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. Evidence for eomesodermin-expressing innate-like CD8(+) KIR/NKG2A(+) T cells in human adults and cord blood samples.
    Authors: Jacomet F, Cayssials E, Basbous S, Levescot A, Piccirilli N, Desmier D, Robin A, Barra A, Giraud C, Guilhot F, Roy L, Herbelin A, Gombert J
    Eur J Immunol, 2015;45(7):1926-33.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. Reversal of tumor immune inhibition using a chimeric cytokine receptor.
    Authors: Leen A, Sukumaran S, Watanabe N, Mohammed S, Keirnan J, Yanagisawa R, Anurathapan U, Rendon D, Heslop H, Rooney C, Brenner M, Vera J
    Mol Ther, 2014;22(6):1211-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  25. Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
    Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
    J Immunol Res, 2014;2014(0):593562.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. Wilms' Tumour 1 (WT1) peptide vaccination in patients with acute myeloid leukaemia induces short-lived WT1-specific immune responses.
    Authors: Uttenthal B, Martinez-Davila I, Ivey A, Craddock C, Chen F, Virchis A, Kottaridis P, Grimwade D, Khwaja A, Stauss H, Morris E
    Br J Haematol, 2014;164(3):366-75.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  27. Concurrent interaction of DCs with CD4(+) and CD8(+) T cells improves secondary CTL expansion: It takes three to tango.
    Authors: Hoyer S, Prommersberger S, Pfeiffer I, Schuler-Thurner B, Schuler G, Dorrie J, Schaft N
    Eur J Immunol, 2014;44(12):3543-59.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  28. Defective expression of scavenger receptors in celiac disease mucosa.
    Authors: Cupi M, Sarra M, De Nitto D, Franze E, Marafini I, Monteleone I, Del Vecchio Blanco G, Paoluzi O, Di Fusco D, Gentileschi P, Ortenzi A, Colantoni A, Pallone F, Monteleone G
    PLoS ONE, 2014;9(6):e100980.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  29. The IL-23/Th17 axis is involved in the adaptive immune response to Bacillus anthracis in humans.
    Authors: Harris K, Ramachandran G, Basu S, Rollins S, Mann D, Cross A
    Eur J Immunol, 2014;44(3):752-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  30. B-cell receptor pathway inhibitors affect CD20 levels and impair antitumor activity of anti-CD20 monoclonal antibodies.
    Authors: Bojarczuk K, Siernicka M, Dwojak M, Bobrowicz M, Pyrzynska B, Gaj P, Karp M, Giannopoulos K, Efremov D, Fauriat C, Golab J, Winiarska M
    Leukemia, 2014;28(5):1163-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  31. Nanoparticle-mediated combinatorial targeting of multiple human dendritic cell (DC) subsets leads to enhanced T cell activation via IL-15-dependent DC crosstalk.
    Authors: Sehgal K, Ragheb R, Fahmy T, Dhodapkar M, Dhodapkar K
    J Immunol, 2014;193(5):2297-305.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  32. Building and optimizing a virus-specific T cell receptor library for targeted immunotherapy in viral infections.
    Authors: Banu, Nasirah, Chia, Adeline, Ho, Zi Zong, Garcia, Alfonso, Paravasivam, Komathi, Grotenbreg, Gijsbert, Bertoletti, Antonio, Gehring, Adam J
    Sci Rep, 2014;4(0):4166.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  33. Thymic stromal lymphopoietin links keratinocytes and dendritic cell-derived IL-23 in patients with psoriasis.
    Authors: Volpe E, Pattarini L, Martinez-Cingolani C, Meller S, Donnadieu M, Bogiatzi S, Fernandez M, Touzot M, Bichet J, Reyal F, Paronetto M, Chiricozzi A, Chimenti S, Nasorri F, Cavani A, Kislat A, Homey B, Soumelis V
    J Allergy Clin Immunol, 2014;134(2):373-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  34. Regulation of CD4(+)NKG2D(+) Th1 cells in patients with metastatic melanoma treated with sorafenib: role of IL-15Ralpha and NKG2D triggering.
    Authors: Romero A, Chaput N, Poirier-Colame V, Rusakiewicz S, Jacquelot N, Chaba K, Mortier E, Jacques Y, Caillat-Zucman S, Flament C, Caignard A, Messaoudene M, Auperin A, Vielh P, Dessen P, Porta C, Mateus C, Ayyoub M, Valmori D, Eggermont A, Robert C, Zitvogel L
    Cancer Res, 2014;74(1):68-80.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  35. CD8+ T cells are activated in an antigen-independent manner in HIV-infected individuals.
    Authors: Bastidas S, Graw F, Smith M, Kuster H, Gunthard H, Oxenius A
    J Immunol, 2014;192(4):1732-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  36. IL-15 prolongs CD154 expression on human CD4 T cells via STAT5 binding to the CD154 transcriptional promoter.
    Authors: Lowe R, Genin A, Orgun N, Cron R
    Genes Immun, 2014;15(3):137-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  37. Hypercytotoxicity and rapid loss of NKp44+ innate lymphoid cells during acute SIV infection.
    Authors: Li H, Richert-Spuhler L, Evans T, Gillis J, Connole M, Estes J, Keele B, Klatt N, Reeves R
    PLoS Pathog, 2014;10(12):e1004551.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Bioassay
  38. Human AdV-specific T cells: persisting in vitro functionality despite lethal irradiation.
    Authors: Geyeregger R, Freimuller C, Stemberger J, Fischer G, Witt V, Fritsch G
    Bone Marrow Transplant, 2014;49(7):934-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  39. Extracellular HCV-core protein induces an immature regulatory phenotype in NK cells: implications for outcome of acute infection.
    Authors: Golden-Mason L, Hahn Y, Strong M, Cheng L, Rosen H
    PLoS ONE, 2014;9(7):e103219.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  40. IL-15 positively regulates IL-21 production in celiac disease mucosa.
    Authors: Sarra M, Cupi M, Monteleone I, Franze E, Ronchetti G, Di Sabatino A, Gentileschi P, Franceschilli L, Sileri P, Sica G, Del Vecchio Blanco G, Cretella M, Paoluzi O, Corazza G, Pallone F, Monteleone G
    Mucosal Immunol, 2013;6(2):244-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  41. The microenvironment of human neuroblastoma supports the activation of tumor-associated T lymphocytes.
    Authors: Carlson L, De Geer A, Sveinbjornsson B, Orrego A, Martinsson T, Kogner P, Levitskaya J
    Oncoimmunology, 2013;2(3):e23618.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  42. GITR-dependent regulation of 4-1BB expression: implications for T cell memory and anti-4-1BB-induced pathology.
    Authors: Lin G, Snell L, Wortzman M, Clouthier D, Watts T
    J Immunol, 2013;190(9):4627-39.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  43. Immunoregulatory pathways are active in the small intestinal mucosa of patients with potential celiac disease.
    Authors: Borrelli M, Salvati V, Maglio M, Zanzi D, Ferrara K, Santagata S, Ponticelli D, Aitoro R, Mazzarella G, Lania G, Gianfrani C, Auricchio R, Troncone R
    Am J Gastroenterol, 2013;108(11):1775-84.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  44. Short-term in-vitro expansion improves monitoring and allows affordable generation of virus-specific T-cells against several viruses for a broad clinical application.
    Authors: Geyeregger R, Freimuller C, Stevanovic S, Stemberger J, Mester G, Dmytrus J, Lion T, Rammensee H, Fischer G, Eiz-Vesper B, Lawitschka A, Matthes S, Fritsch G
    PLoS ONE, 2013;8(4):e59592.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  45. PaCS is a novel cytoplasmic structure containing functional proteasome and inducible by cytokines/trophic factors.
    Authors: Sommi, Patrizia, Necchi, Vittorio, Vitali, Agostina, Montagna, Daniela, De Luigi, Ada, Salmona, Mario, Ricci, Vittorio, Solcia, Enrico
    PLoS ONE, 2013;8(12):e82560.
    Species: Human
    Sample Type: Whole Cells
    Application: Cell Culture Substrate
  46. Loss of methylation at the IFNG promoter and CNS-1 is associated with the development of functional IFN-gamma memory in human CD4(+) T lymphocytes.
    Authors: Dong J, Chang H, Ivascu C, Qian Y, Rezai S, Okhrimenko A, Cosmi L, Maggi L, Eckhardt F, Wu P, Sieper J, Alexander T, Annunziato F, Gossen M, Li J, Radbruch A, Thiel A
    Eur J Immunol, 2013;43(3):793-804.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  47. CCR5 is a receptor for Staphylococcus aureus leukotoxin ED.
    Authors: Alonzo, Francis, Kozhaya, Lina, Rawlings, Stephen, Reyes-Robles, Tamara, DuMont, Ashley L, Myszka, David G, Landau, Nathanie, Unutmaz, Derya, Torres, Victor J
    Nature, 2013;493(7430):51-5.
    Species: Human
    Sample Type: Whole Cells
    Application: Cell Culture Substrate
  48. 2-deoxy D-glucose prevents cell surface expression of NKG2D ligands through inhibition of N-linked glycosylation.
    Authors: Andresen L, Skovbakke SL, Persson G, Hagemann-Jensen M, Hansen KA, Jensen H, Skov S
    J. Immunol., 2012;188(4):1847-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  49. Antigen-independent induction of Tim-3 expression on human T cells by the common gamma-chain cytokines IL-2, IL-7, IL-15, and IL-21 is associated with proliferation and is dependent on the phosphoinositide 3-kinase pathway.
    Authors: Mujib S, Jones RB, Lo C, Aidarus N, Clayton K, Sakhdari A, Benko E, Kovacs C, Ostrowski MA
    J. Immunol., 2012;188(8):3745-56.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  50. The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells.
    Authors: Ichii, Michiko, Frank, Mark Bar, Iozzo, Renato V, Kincade, Paul W
    Blood, 2012;119(7):1683-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  51. Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
    Authors: Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S
    PLoS Pathog., 2012;8(1):e1002486.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  52. Human breast cancer cells enhance self tolerance by promoting evasion from NK cell antitumor immunity.
    Authors: Mamessier E, Sylvain A, Thibult ML, Houvenaeghel G, Jacquemier J, Castellano R, Goncalves A, Andre P, Romagne F, Thibault G, Viens P, Birnbaum D, Bertucci F, Moretta A, Olive D
    J. Clin. Invest., 2011;121(9):3609-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  53. IL-17-producing invariant NKT cells in lymphoid organs are recent thymic emigrants identified by neuropilin-1 expression.
    Authors: Milpied P, Massot B, Renand A, Diem S, Herbelin A, Leite-de-Moraes M, Rubio MT, Hermine O
    Blood, 2011;118(11):2993-3002.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  54. CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response.
    Authors: Zhang HH, Song K, Rabin RL, Hill BJ, Perfetto SP, Roederer M, Douek DC, Siegel RM, Farber JM
    J. Immunol., 2010;185(11):6646-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  55. Barley as a green factory for the production of functional Flt3 ligand.
    Authors: Erlendsson LS, Muench MO, Hellman U, Hrafnkelsdottir SM, Jonsson A, Balmer Y, Mantyla E, Orvar BL
    Biotechnol J, 2010;5(2):163-71.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  56. Natural killer cell-mediated cytokine response among HIV-positive south Indians with pulmonary tuberculosis.
    Authors: Ramana Rao PV, Rajasekaran S, Raja A
    J. Interferon Cytokine Res., 2010;30(1):33-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  57. Development of functional human NK cells in an immunodeficient mouse model with the ability to provide protection against tumor challenge.
    Authors: Kwant-Mitchell A, Pek EA, Rosenthal KL
    PLoS ONE, 2009;4(12):e8379.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  58. Dermatan sulfate proteoglycan biglycan as a potential selectin L/CD44 ligand involved in selective recruitment of peripheral blood CD16(-) natural killer cells into human endometrium.
    Authors: Kitaya K, Yasuo T
    J. Leukoc. Biol., 2009;85(3):391-400.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  59. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
    Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
    Circulation, 2009;119(10):1424-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  60. EBV-associated mononucleosis does not induce long-term global deficit in T-cell responsiveness to IL-15.
    Authors: Giron-Michel J, Menard F, Negrini S, Devocelle A, Azzarone B, Besson C
    Blood, 2009;113(19):4541-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  61. Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages.
    Authors: Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P
    Int. J. Cancer, 2009;125(2):367-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  62. CD27 defines phenotypically and functionally different human NK cell subsets.
    Authors: Vossen MT, Matmati M, Hertoghs KM, Baars PA, Gent MR, Leclercq G, Hamann J, Kuijpers TW, van Lier RA
    J. Immunol., 2008;180(6):3739-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  63. Mouse fetal and embryonic liver cells differentiate human umbilical cord blood progenitors into CD56-negative natural killer cell precursors in the absence of interleukin-15.
    Authors: McCullar V, Oostendorp R, Panoskaltsis-Mortari A, Yun G, Lutz CT, Wagner JE, Miller JS
    Exp. Hematol., 2008;36(5):598-608.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  64. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  65. Soluble Interleukin IL-15Ralpha is generated by alternative splicing or proteolytic cleavage and forms functional complexes with IL-15.
    Authors: Bulanova E, Budagian V, Duitman E, Orinska Z, Krause H, Ruckert R, Reiling N, Bulfone-Paus S
    J. Biol. Chem., 2007;282(18):13167-79.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  66. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  67. Activating and inhibitory receptors on synovial fluid natural killer cells of arthritis patients: role of CD94/NKG2A in control of cytokine secretion.
    Authors: de Matos CT, Berg L, Michaelsson J, Fellander-Tsai L, Karre K, Soderstrom K
    Immunology, 2007;122(2):291-301.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  68. Transforming growth factor beta 1(TGF-beta1) down-regulates TNFalpha-induced RANTES production in rheumatoid synovial fibroblasts through NF-kappaB-mediated transcriptional repression.
    Authors: Cho ML, Min SY, Chang SH, Kim KW, Heo SB, Lee SH, Park SH, Cho CS, Kim HY
    Immunol. Lett., 2006;105(2):159-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  69. Human plasmacytoid predendritic cells activate NK cells through glucocorticoid-induced tumor necrosis factor receptor-ligand (GITRL).
    Authors: Hanabuchi S, Watanabe N, Wang YH, Ito T, Shaw J, Cao W, Qin FX, Liu YJ
    Blood, 2006;107(9):3617-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  70. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006;241(1):7-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  71. A novel bispecific protein (ULBP2-BB4) targeting the NKG2D receptor on natural killer (NK) cells and CD138 activates NK cells and has potent antitumor activity against human multiple myeloma in vitro and in vivo.
    Authors: von Strandmann EP, Hansen HP, Reiners KS, Schnell R, Borchmann P, Merkert S, Simhadri VR, Draube A, Reiser M, Purr I, Hallek M, Engert A
    Blood, 2006;107(5):1955-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  72. Familial NK cell deficiency associated with impaired IL-2- and IL-15-dependent survival of lymphocytes.
    Authors: Eidenschenk C, Jouanguy E, Alcais A, Mention JJ, Pasquier B, Fleckenstein IM, Puel A, Gineau L, Carel JC, Vivier E, Le Deist F, Casanova JL
    J. Immunol., 2006;177(12):8835-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  73. The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors.
    Authors: Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS
    J. Immunol., 2006;177(7):4488-94.
    Species: Human
    Sample Type: Whole Tissue
    Application: Bioassay
  74. Bovine splenic NK cells synthesize IFN-gamma in response to IL-12-containing supernatants from Babesia bovis-exposed monocyte cultures.
    Authors: Goff WL, Storset AK, Johnson WC, Brown WC
    Parasite Immunol., 2006;28(5):221-8.
    Species: Bovine
    Sample Type: Whole Cells
    Application: Bioassay
  75. IL-21 sustains CD28 expression on IL-15-activated human naive CD8+ T cells.
    Authors: Alves NL, Arosa FA, van Lier RA
    J. Immunol., 2005;175(2):755-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  76. Renal cells express a functional interleukin-15 receptor.
    Authors: Tejman-Yarden N, Zlotnik M, Lewis E, Etzion O, Chaimovitz C, Douvdevani A
    Nephrol. Dial. Transplant., 2005;20(3):516-23.
    Species: N/A
    Sample Type: N/A
    Application: WB Ctrl
  77. Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
    Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
    J. Immunol., 2005;174(4):2376-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  78. Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling.
    Authors: Yamasaki S, Maeda M, Ohshima K, Kikuchi M, Otsuka T, Harada M
    Leuk. Res., 2004;28(10):1023-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  79. The JAK3 inhibitor CP-690550 selectively reduces NK and CD8+ cell numbers in cynomolgus monkey blood following chronic oral dosing.
    Authors: Conklyn M, Andresen C, Changelian P, Kudlacz E
    J. Leukoc. Biol., 2004;76(6):1248-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  80. Expression and activity of IL-17 in cutaneous T-cell lymphomas (mycosis fungoides and Sezary syndrome).
    Authors: Ciree A, Michel L, Camilleri-Broet S, Jean Louis F, Oster M, Flageul B, Senet P, Fossiez F, Fridman WH, Tartour E
    Int. J. Cancer, 2004;112(1):113-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  81. IL-21 induces the functional maturation of murine NK cells.
    Authors: Brady J, Hayakawa Y, Smyth MJ, Nutt SL
    J. Immunol., 2004;172(4):2048-58.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  82. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
    Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
    J. Immunol., 2004;173(5):3482-91.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  83. Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis.
    Authors: Groh V, Bruhl A, El-Gabalawy H, Nelson JL, Spies T
    Proc. Natl. Acad. Sci. U.S.A., 2003;100(16):9452-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  84. Use of interleukin 15 to enhance interferon-gamma production by antigen-specific stimulated lymphocytes from rhesus macaques.
    Authors: Calarota SA, Otero M, Hermanstayne K, Lewis M, Rosati M, Felber BK, Pavlakis GN, Boyer JD, Weiner DB
    J. Immunol. Methods, 2003;279(1):55-67.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Bioassay
  85. Regulation of CCR5 expression and MIP-1alpha production in CD4+ T cells from patients with rheumatoid arthritis.
    Authors: Wang CR, Liu MF
    Clin. Exp. Immunol., 2003;132(2):371-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  86. Decreased IL-15 may contribute to elevated IgE and acute inflammation in atopic dermatitis.
    Authors: Ong PY, Hamid QA, Travers JB, Strickland I, Al Kerithy M, Boguniewicz M, Leung DY
    J. Immunol., 2002;168(1):505-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  87. Restoration of cytotoxic T lymphocyte function in malignant pleural effusion: interleukin-15 vs. interleukin-2.
    Authors: Chen YM, Ting CC, Peng JW, Yang WK, Yang KY, Tsai CM, Perng RP
    J. Interferon Cytokine Res., 2000;20(1):31-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  88. Human amniotic fluid lacks interleukin-2 and interleukin-15 but can interact with the beta-chain of the interleukin-2 receptor.
    Authors: Searle RF, Bromage SJ, Palmer J, Curry JE, Lang AK
    Immunology, 2000;99(3):411-7.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  89. Dendritic Cell-Derived IL-32alpha: A Novel Inhibitory Cytokine of NK Cell Function.
    Authors: Gorvel L, Korenfeld D, Tung T, Klechevsky E
    J Immunol, 0;199(4):1290-1300.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 89 Citations
Average Rating: 5 (Based on 1 review)

Have you used Recombinant Human IL-15 Protein?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Recombinant Human IL-15 Protein.

We have 1 review tested in 1 application: Immunoassay Standard.
Filter by Ratings
Excellent
(1)
All Ratings
Filter by Applications
Immunoassay Standard
(1)
All Applications
Filter by Species
All Species

Images Ratings Applications Species Reviewed By Date Details
Immunoassay Standard Recombinant Human IL-15 Protein 247-ILB
Enlarge
Excellent
 Immunoassay Standard Anonymous 11/20/2017
Show
Image Details
Immunoassay Standard Recombinant Human IL-15 Protein 247-ILB
Immunoassay Standard: Recombinant Human IL-15 Protein [247-ILB]

Summary

ApplicationImmunoassay Standard

Other Experimental Details

Other Experimental DetailsThe IL15 protein was used as a control and standard for an ELISA using MAB647 and BAM247 as the matched pair.

Order Details

  • Formulations

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information