Recombinant Human IL-15R alpha Fc Chimera (HEK293), CF

Catalog # Availability Size / Price Qty
7194-IR-050
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Recombinant Human IL-15R alpha Fc Chimera (HEK293), CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to block human IL-15-induced proliferation of CTLL‑2 mouse cytotoxic T cells. Ruchatz, H. et al. (1998) J. Immunol. 160:5654.

The ED50 for this effect is 2-10 ng/mL.

Source
Human embryonic kidney cell, HEK293-derived human IL-15 R alpha protein
Human IL-15 R alpha
(Met1-Thr205)
Accession # Q13261
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ile31
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
45 kDa (monomer)
SDS-PAGE
75-90 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7194-IR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IL-15R alpha

Interleukin 15 Receptor alpha (IL‑15 R alpha ), also known as CD215, is a widely expressed 60 kDa transmembrane glycoprotein that plays an important role in the homeostasis and activation of NK cells and CD8+ memory T cells and participates in the development and function of many other hematopoietic cell types and non‑immune cell types (1 ‑ 3). Mature human IL‑15 R alpha consists of a 175 aa extracellular domain (ECD) containing one N‑linked glycosylation site, a 23 aa transmembrane segment, and a 39 aa cytoplasmic tail (4). Within the ECD, human IL‑15 R alpha shares approximately 60% aa sequence identity with mouse and rat IL‑15 R alpha. Alternate splicing of human IL‑15 R alpha generates additional isoforms with variable length deletions in the ECD and/or substitutions in the cytoplasmic domain (4, 5). IL‑15 R alpha binds to Interleukin‑15 with high affinity (6). IL‑15 additionally interacts with lower affinity to a complex of IL‑2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL‑2 receptor complex (7, 8). The use of shared receptor components contributes to the overlapping biological effects of IL‑15 and IL‑2. The dominant mechanism of IL‑15 action is known as transpresentation in which IL‑15/IL‑15 R alpha complexes are expressed on the surface of one cell and interact with complexes of IL‑2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL‑15 even if they do not express IL‑15 R alpha (10 ‑ 12). IL‑15/IL‑15 R alpha complexes can transmit reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL‑15/IL‑15 R alpha expressing cells (13, 14). Shed soluble forms of IL‑15 R alpha retain the ability to bind tightly to IL‑15 and can inhibit IL‑15 bioactivity (6, 15, 16).

References
  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Di Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
  3. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
  4. Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
  5. Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.
  6. Giri, J.G. et al. (1995) EMBO 14:3654.
  7. Grabstein, K. et al. (1994) Science 264:965.
  8. Giri, J. et al. (1994) EMBO J. 13:2822.
  9. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
  10. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
  11. Dubois, S. et al. (2002) Immunity 17:537.
  12. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
  13. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  14. Neely, G.G. et al. (2004) J. Immunol. 172:4225.
  15. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  16. Mortier, E. et al. (2004) J. Immunol. 173:1681.
Long Name
Interleukin 15 Receptor alpha
Entrez Gene IDs
3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)
Alternate Names
CD215 antigen; CD215; IL15 R alpha; IL-15 R alpha; IL-15 receptor subunit alpha; IL-15R alpha; IL15RA; IL-15Ra; IL-15R-alpha; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha; MGC104179

Citations for Recombinant Human IL-15R alpha Fc Chimera (HEK293), CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Design and characterisation of a novel interleukin-15 receptor alpha fusion protein and analysis of interleukin-15 complexation
    Authors: AS Schmid, D Neri
    PLoS ONE, 2019;14(7):e0219313.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Discovery and characterization of a novel humanized anti-IL-15 antibody and its relevance for the treatment of refractory celiac disease and eosinophilic esophagitis
    Authors: AP Vicari, AM Schoepfer, B Meresse, L Goffin, O Léger, S Josserand, N Guégan, S Yousefi, A Straumann, N Cerf-Bensu, HU Simon, Y Chvatchko
    MAbs, 2017;0(0):0.
    Species: Human
    Sample Types: Protein

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human IL-15R alpha Fc Chimera (HEK293), CF

There are currently no reviews for this product. Be the first to review Recombinant Human IL-15R alpha Fc Chimera (HEK293), CF and earn rewards!

Have you used Recombinant Human IL-15R alpha Fc Chimera (HEK293), CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review