Recombinant Human IL-17 RC Isoform 1 Fc Chimera Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human IL-17RC Isoform 1 Fc Chimera (Catalog # 9284-IL) is coated at 0.5 µg/mL, Recombinant Human IL-17/IL-17A (Catalog # 7955-IL) binds with an ED50 = 0.1-0.6 ng/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived
    Human IL-17 RC Isoform 1
    (Leu21-His465)
    Accession # NP_703190.1
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu21
  • Predicted Molecular Mass
    76 kDa
  • SDS-PAGE
    95-113 kDa, reducing conditions
9284-IL
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Human
IL-17 RC Isoform 1 Fc Chimera (Catalog #
9284-IL) is coated at 0.5 µg/mL, Recombinant Human IL-17/IL-17A (Catalog # 7955-IL) binds with an ED50 = 0.1-0.6 ng/mL.
Background: IL-17 RC
IL-17 receptor C (IL-17RC; also known as IL-17RL and IL-17Rhom) is an 85-110 kDa member of the IL-17 receptor family. IL-17 plays a key role in the development of autoimmunity and inflammation (1, 2). Human IL-17 RC is a type I transmembrane glycoprotein that is expressed on neutrophils, endothelial cells, chondrocytes and osteoblasts, breast and prostatic epithelium, fibroblasts, renal tubular epithelium, and skeletal muscle cells (3-5). Mature human IL-17 RC consists of a 518 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 232 aa cytoplasmic region (6). It is estimated that there are over 90 alternative splice forms, with transmembrane-containing isoforms predominating (4, 7). This recombinant protein corresponds to IL-17 RC isoform 1, the canonical form. Within the ECD, IL-17 RC isoform 1 shares 69% aa sequence identity with mouse IL-17 RC. IL-17 RC is the cognate receptor for IL-17F, and it binds IL-17A with similar affinity (8). IL-17 RC associates wiith IL-17 RA to form a receptor complex for both IL-17A and IL-17F (8-11).
  • References:
    1. Song, X. and Y. Qian (2013) Cytokine 62:175.
    2. Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:821.
    3. Taylor, P.R. et al. (2014) Nat. Immunol. 15:143.
    4. You, Z. et al. (2007) Neoplasia 9:464.
    5. Kokubu, T. et al. (2008) J. Hictochem. Cytochem. 56:89.
    6. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
    7. Haudenschild, D.R. et al. (2006) Prostate 66:1268.
    8. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
    9. Toy, D. et al. (2006) J. Immunol. 177:36.
    10. Kramer, J.M. et al. (2006) J. Immunol. 176:711.
    11. Kramer, J.M. et al. (2007) J. Immunol. 179:6379.
  • Long Name:
    Interleukin 17 Receptor C, Transcript Variant 3
  • Entrez Gene IDs:
    84818 (Human); 171095 (Mouse)
  • Alternate Names:
    FLJ95963; FLJ96005; IL-17 RC; IL-17 receptor C; IL-17 RL; IL17F receptor; IL17RC; IL-17RC; IL17Rhom; IL17RL; IL-17RL; IL17-RL; interleukin 17 receptor C; interleukin-17 receptor C; Interleukin-17 receptor homolog; Interleukin-17 receptor-like protein; MGC10763; zcytoR14
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