Recombinant Human IL-1ra/IL-1F3 Protein

Formulations:
Catalog # Availability Size / Price Qty
280-RA-010
280-RA-050
Recombinant Human IL-1ra/IL-1F3 Protein Data
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Product Details
Citations (44)
FAQs
Reviews (3)

Recombinant Human IL-1ra/IL-1F3 Protein Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-1 alpha -dependent proliferation in D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 5-40 ng/mL in the presence of 50 pg/mL of rhIL-1 alpha.
Source
E. coli-derived human IL-1ra/IL-1F3 protein
Accession #
Predicted Molecular Mass
17 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

280-RA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

280-RA/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA) inhibits IL-1a-dependent cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50for this effect is 5-40 ng/mL in the presence of 50 pg/mL of Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA).

SDS-PAGE View Larger

1 μg/lane of Recombinant Human IL-1ra/IL-1F3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 20 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1ra/IL-1F3

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

Long Name
Interleukin 1 Receptor Antagonist
Entrez Gene IDs
3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine)
Alternate Names
DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4

Citations for Recombinant Human IL-1ra/IL-1F3 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Mycobacterium tuberculosis Lipoarabinomannan Activates Human Neutrophils via a TLR2/1 Mechanism Distinct from Pam3CSK4
    Authors: JS Hook, M Cao, K Weng, N Kinnare, JG Moreland
    J. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Standard
  2. IL-1RA is part of the inflammasome-regulated immune response in bladder epithelial cells and influences colonization of uropathogenic E. coli
    Authors: A Lindblad, K Persson, I Demirel
    Cytokine, 2019;123(0):154772.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Osmotic induction of cyclooxygenase-2 in RPE cells: Stimulation of inflammasome activation
    Authors: L Messerschm, S Fischer, P Wiedemann, A Bringmann, M Hollborn
    Mol. Vis., 2019;25(0):329-344.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Transition from metal-DTH resistance to susceptibility is facilitated by NLRP3 inflammasome signaling induced Th17 reactivity: Implications for orthopedic implants
    Authors: L Samelko, MS Caicedo, J Jacobs, NJ Hallab
    PLoS ONE, 2019;14(1):e0210336.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Human Scavenger Receptor A1-Mediated Inflammatory Response to Silica Particle Exposure Is Size Specific
    Authors: N Nishijima, T Hirai, K Misato, M Aoyama, E Kuroda, KJ Ishii, K Higashisak, Y Yoshioka, Y Tsutsumi
    Front Immunol, 2017;8(0):379.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Lysosomal destabilization activates the NLRP3 inflammasome in human umbilical vein endothelial cells (HUVECs)
    Authors: K Kinnunen, N Piippo, S Loukovaara, M Hytti, K Kaarnirant, A Kauppinen
    J Cell Commun Signal, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Advanced glycation end products regulate anabolic and catabolic activities via NLRP3-inflammasome activation in human nucleus pulposus cells
    Authors: Y Song, Y Wang, Y Zhang, W Geng, W Liu, Y Gao, S Li, K Wang, X Wu, L Kang, C Yang
    J. Cell. Mol. Med, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. A Functional Genomics Approach to Understand Variation in Cytokine Production in Humans
    Authors: Mihai G Netea
    Cell, 2016;167(4):1099-1110.e14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation
    Sci Rep, 2016;6(0):25171.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Interleukin-1- and type I interferon-dependent enhanced immunogenicity of an NYVAC-HIV-1 Env-Gag-Pol-Nef vaccine vector with dual deletions of type I and type II interferon-binding proteins.
    Authors: Delaloye J, Filali-Mouhim A, Cameron M, Haddad E, Harari A, Goulet J, Gomez C, Perdiguero B, Esteban M, Pantaleo G, Roger T, Sekaly R, Calandra T
    J Virol, 2015;89(7):3819-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha.
    Authors: Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P
    Eur J Immunol, 2015;45(4):1092-102.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Type I IFNs and IL-18 regulate the antiviral response of primary human gammadelta T cells against dendritic cells infected with Dengue virus.
    Authors: Tsai C, Liong K, Gunalan M, Li N, Lim D, Fisher D, Macary P, Leo Y, Wong S, Puan K, Wong S
    J Immunol, 2015;194(8):3890-900.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Activation of toll like receptor 4 attenuates GABA synthesis and postsynaptic GABA receptor activities in the spinal dorsal horn via releasing interleukin-1 beta.
    Authors: Yan X, Jiang E, Weng H
    J Neuroinflammation, 2015;12(0):222.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  14. Pro-Inflammatory Cytokine IL-1beta Up-Regulates CXC Chemokine Receptor 4 via Notch and ERK Signaling Pathways in Tongue Squamous Cell Carcinoma.
    Authors: Sun Y, Zhu D, Wang G, Wang D, Zhou H, Liu X, Jiang M, Liao L, Zhou Z, Hu J
    PLoS ONE, 2015;10(7):e0132677.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells.
    Authors: Sugita S, Kamao H, Iwasaki Y, Okamoto S, Hashiguchi T, Iseki K, Hayashi N, Mandai M, Takahashi M
    Invest Ophthalmol Vis Sci, 2015;56(2):1051-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Cholera toxin, and the related nontoxic adjuvants mmCT and dmLT, promote human Th17 responses via cyclic AMP-protein kinase A and inflammasome-dependent IL-1 signaling.
    Authors: Larena M, Holmgren J, Lebens M, Terrinoni M, Lundgren A
    J Immunol, 2015;194(8):3829-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. IL-1alpha released from damaged epithelial cells is sufficient and essential to trigger inflammatory responses in human lung fibroblasts.
    Authors: Suwara M, Green N, Borthwick L, Mann J, Mayer-Barber K, Barron L, Corris P, Farrow S, Wynn T, Fisher A, Mann D
    Mucosal Immunol, 2014;7(3):684-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Interleukin-1beta-induced Wnt5a enhances human corneal endothelial cell migration through regulation of Cdc42 and RhoA.
    Authors: Lee J, Heur M
    Mol Cell Biol, 2014;34(18):3535-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Interleukin-33 induces urokinase in human endothelial cells--possible impact on angiogenesis.
    Authors: Stojkovic S, Kaun C, Heinz M, Krychtiuk K, Rauscher S, Lemberger C, de Martin R, Groger M, Petzelbauer P, Huk I, Huber K, Wojta J, Demyanets S
    J Thromb Haemost, 2014;12(6):948-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. IL-18 regulates melanoma VLA-4 integrin activation through a Hierarchized sequence of inflammatory factors.
    Authors: Valcarcel M, Carrascal T, Crende O, Vidal-Vanaclocha F
    J Invest Dermatol, 2014;134(2):470-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Detailed mechanistic analysis of gevokizumab, an allosteric anti-IL-1beta antibody with differential receptor-modulating properties.
    Authors: Issafras H, Corbin J, Goldfine I, Roell M
    J Pharmacol Exp Ther, 2014;348(1):202-15.
    Species: N/A
    Sample Types: Protein
    Applications: Binding Assay
  22. Interleukin-6 mediates epithelial-stromal interactions and promotes gastric tumorigenesis.
    Authors: Kinoshita H, Hirata Y, Nakagawa H, Sakamoto K, Hayakawa Y, Takahashi R, Nakata W, Sakitani K, Serizawa T, Hikiba Y, Akanuma M, Shibata W, Maeda S, Koike K
    PLoS ONE, 2013;8(4):e60914.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  23. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
    Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
    PLoS Biol, 2012;10(9):e1001395.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Persistent Helicobacter pylori Specific Th17 Responses in Patients with Past H. pylori Infection Are Associated with Elevated Gastric Mucosal IL-1β.
    Authors: Serelli-Lee V, Ling KL, Ho C, Yeong LH, Lim GK, Ho B, Wong SB
    PLoS ONE, 2012;7(6):e39199.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  25. Regulating human Th17 cells via differential expression of IL-1 receptor.
    Authors: Lee WW, Kang SW, Choi J
    Blood, 2010;115(3):530-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
  27. Mechanisms underlying the nociceptive responses induced by platelet-activating factor (PAF) in the rat paw.
    Authors: Marotta DM, Costa R, Motta EM, Fernandes ES, Medeiros R, Quintao NL, Campos MM, Calixto JB
    Biochem. Pharmacol., 2009;77(7):1223-35.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  28. Interleukin-1 mediates the anorexic and febrile actions of galanin-like Peptide.
    Authors: Man PS, Lawrence CB
    Endocrinology, 2008;149(11):5791-802.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  29. P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
    Authors: Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC
    Br. J. Pharmacol., 2007;151(1):115-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Tumour necrosis factor-alpha (TNF-alpha) increases nuclear factor kappaB (NFkappaB) activity in and interleukin-8 (IL-8) release from bovine mammary epithelial cells.
    Authors: Fitzgerald DC, Meade KG, McEvoy AN, Lillis L, Murphy EP, Machugh DE, Baird AW
    Vet. Immunol. Immunopathol., 2007;116(1):59-68.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
    Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
    Int. J. Cancer, 2006;119(10):2313-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. HIV-1-infected and/or immune activated macrophages regulate astrocyte SDF-1 production through IL-1beta.
    Authors: Peng H, Erdmann N, Whitney N, Dou H, Gorantla S, Gendelman HE, Ghorpade A, Zheng J
    Glia, 2006;54(6):619-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. The response of human epithelial cells to TNF involves an inducible autocrine cascade.
    Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK
    Cell, 2006;124(6):1225-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Low concentration of interleukin-1beta induces FLICE-inhibitory protein-mediated beta-cell proliferation in human pancreatic islets.
    Authors: Maedler K, Schumann DM, Sauter N, Ellingsgaard H, Bosco D, Baertschiger R, Iwakura Y, Oberholzer J, Wollheim CB, Gauthier BR, Donath MY
    Diabetes, 2006;55(10):2713-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. IL-22 increases the innate immunity of tissues.
    Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
    Immunity, 2004;21(2):241-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Proline-rich tyrosine kinase 2 and Src kinase signaling transduce monosodium urate crystal-induced nitric oxide production and matrix metalloproteinase 3 expression in chondrocytes.
    Authors: Liu R, Liote F, Rose DM, Merz D, Terkeltaub R
    Arthritis Rheum., 2004;50(1):247-58.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  37. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
    Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
    J. Immunol., 2004;173(5):3482-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Interleukin-1 inhibits voltage-dependent P/Q-type Ca2+ channel associated with the inhibition of the rise of intracellular free Ca2+ concentration and catecholamine release in adrenal chromaffin cells.
    Authors: Morita K, Miyasako T, Kitayama S, Dohi T
    Biochim. Biophys. Acta, 2004;1673(3):160-9.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  39. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004;284(1):99-106.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  40. The effect of inflammatory cytokines on secretion of macrophage colony-stimulating factor and monocyte chemoattractant protein-1 in human granulosa cells.
    Authors: Kawano Y, Fukuda J, Itoh H, Takai N, Nasu K, Miyakawa I
    Am. J. Reprod. Immunol., 2004;52(2):124-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Interleukin-8 production in tuberculous pleurisy: role of mesothelial cells stimulated by cytokine network involving tumour necrosis factor-alpha and interleukin-1 beta.
    Authors: Park JS, Kim YS, Jee YK, Myong NH, Lee KY
    Scand. J. Immunol., 2003;57(5):463-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  42. Fas and Fas-associated death domain protein regulate monocyte chemoattractant protein-1 expression by human smooth muscle cells through caspase- and calpain-dependent release of interleukin-1alpha.
    Authors: Schaub FJ, Liles WC, Ferri N, Sayson K, Seifert RA, Bowen-Pope DF
    Circ. Res., 2003;93(6):515-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. Activated platelets mediate inflammatory signaling by regulated interleukin 1beta synthesis.
    Authors: Lindemann S, Tolley ND, Dixon DA, McIntyre TM, Prescott SM, Zimmerman GA, Weyrich AS
    J. Cell Biol., 2001;154(3):485-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Interleukin-12 induces sustained activation of multiple host inflammatory mediator systems in chimpanzees.
    Authors: Lauw FN, Dekkers PE, te Velde AA, Speelman P, Levi M, Kurimoto M, Hack CE, Van Deventer SJ, van der Poll T
    J. Infect. Dis., 1999;179(3):646-52.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)

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Reviews for Recombinant Human IL-1ra/IL-1F3 Protein

Average Rating: 5 (Based on 3 Reviews)

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