Recombinant Human IL-2 R beta Protein

Formulations:
Catalog # Availability Size / Price Qty
224-2B-005
224-2B-025
Product Details
Citations (1)
FAQs
Reviews

Recombinant Human IL-2 R beta Protein Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 1.0-3.0 µg/mL in the presence of 4.0 ng/mL of recombinant human IL-15.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-2 R beta protein
Ala27-Asp239
Accession #
N-terminal Sequence
Analysis
Ala27
Predicted Molecular Mass
25 kDa
SDS-PAGE
29 kDa and 31 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

224-2B

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

224-2B/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-2 R beta

Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. Individual beta chains and alpha chains exhibit low affinity IL-2 binding and the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain have been shown to be required for IL-15 mediated signaling.

IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. A soluble IL-2 R beta (IL-2 sR beta ) has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 R beta. At present, the function of IL-2 sR beta is unclear. Recombinant human IL-2 sR beta binds IL-2 with low affinity and is not an effective IL-2 antagonist on cells displaying the high or intermediate affinity IL-2 signaling receptors. Nevertheless, IL-2 sR beta binds IL-15 with sufficient affinity to neutralize IL-15 biological activities.

Long Name
Interleukin 2 Receptor beta
Entrez Gene IDs
3560 (Human); 16185 (Mouse)
Alternate Names
CD122 antigen; CD122; high affinity IL-2 receptor beta subunit; High affinity IL-2 receptor subunit beta; IL-15 R beta; IL-2 R beta; IL-2 receptor subunit beta; IL2R beta; IL-2R subunit beta; IL2RB; IL-2Rb; interleukin 15 receptor, beta; interleukin 2 receptor, beta; interleukin-2 receptor subunit beta; P70-75; p75

Citation for Recombinant Human IL-2 R beta Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Highly potent anti-CD20-RLI immunocytokine targeting established human B lymphoma in SCID mouse.
    Authors: Vincent M, Teppaz G, Lajoie L, Sole V, Bessard A, Maillasson M, Loisel S, Bechard D, Clemenceau B, Thibault G, Garrigue-Antar L, Jacques Y, Quemener A
    MAbs, 2014;6(4):1026-37.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

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