IL-21 (Interleukin-21) is member of the family of cytokines that utilize the common gamma chain as a receptor subunit. This subunit is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells. It exerts its biological effects through a heterodimeric receptor complex of the common gamma chain and the IL-21-specific IL-21 R. IL-21 supports Tfh cell development, immunoglobulin affinity maturation, plasma cell differentiation, B cell memory responses, and the migration of dendritic cells to draining lymph nodes. It costimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization. It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells. In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation.
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Recombinant Human IL-21 Protein, CF
R&D Systems | Catalog # BT-021
Analyzed by SEC-MALS
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Key Product Details
- R&D Systems E. coli-derived Recombinant Human IL-21 Protein (BT-021)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Bioactivity
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Product Specifications
Source
E. coli-derived human IL-21 protein
Gln32-Ser162 with a N-terminal Met
Gln32-Ser162 with a N-terminal Met
Purity
>95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
15 kDa
SDS-PAGE
15-17 kDa, under reducing conditions.
Activity
Measured in a cell proliferation assay using B9 mouse hybridoma cells.
The ED50 for this effect is 5.00-50.0 ng/mL.
The ED50 for this effect is 5.00-50.0 ng/mL.
Reviewed Applications
Read 1 review rated 5 using BT-021 in the following applications:
Scientific Data Images for Recombinant Human IL-21 Protein, CF
Recombinant Human IL‑21 Protein SEC-MALS.
Recombinant Human IL‑21 (Catalog # BT-021) has a molecular weight (MW) of 17.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.
Recombinant Human IL‑21 Protein Bioactivity.
Recombinant Human IL-21 induces cell proliferation of B9 mouse hybridoma cells with an ED50 of 5.00-50.0 ng/mL.
Recombinant Human IL‑21 Protein SDS-PAGE.
2 μg/lane of Recombinant Human IL‑21 Protein (Catalog # BT-021) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 15-17 kDa, under reducing conditions.Formulation, Preparation, and Storage
BT-021
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. |
| Reconstitution | Reconstitute the 10 μg size at 100 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: IL-21
Long Name
Interleukin 21
Alternate Names
CVID11, IL21, Za11
Gene Symbol
IL21
UniProt
Additional IL-21 Products
Product Documents for Recombinant Human IL-21 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human IL-21 Protein, CF
For research use only
Customer Reviews for Recombinant Human IL-21 Protein, CF (1)
5 out of 5
1 Customer Rating
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Application: Cell ProliferationSpecies: HumanVerified Customer | Posted 04/14/2026IL-21 induced lymphocyte proliferationIL-21 induced lymphocyte proliferation
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