Recombinant Human IL-33 Protein

Carrier Free

Catalog # Availability Size / Price Qty
3625-IL-010/CF

With Carrier

Catalog # Availability Size / Price Qty
3625-IL-010
Recombinant Human IL-33 Protein Bioactivity
2 Images
Product Details
Citations (47)
FAQs
Reviews (6)

Recombinant Human IL-33 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.06-0.24 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
E. coli-derived human IL-33 protein
Ser112-Thr270
Accession #
N-terminal Sequence
Analysis
Ser112
Predicted Molecular Mass
18 kDa

Product Datasheets

3625-IL (with carrier)

3625-IL/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3625-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3625-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Human IL-33 Protein Bioactivity View Larger

Recombinant Human IL-33 (Catalog # 3625‑IL) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 0.06-0.24 ng/mL.

SDS-PAGE Recombinant Human IL-33 Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human IL-33 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1‑3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF‑HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL‑33 was identified based on sequence and structural homology with IL‑1 family cytokines (3). DVS 27, NF‑HEV, and IL‑33 share 100% amino acid sequence identity. IL‑33 is constitutively expressed in smooth muscle and airway epithelia. It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL‑1 alpha or IL‑1 beta stimulation (1, 3). Similar to IL‑1, IL‑33 can be cleaved in vitro by caspase‑1, generating an N‑terminal fragment that is slightly shorter than the C‑terminal fragment (3, 4). The N‑terminal portion of full length IL‑33 contains a predicted bipartite nuclear localization sequence and a homeodomain‑like helix‑turn‑helix DNA binding domain. By immunofluorescence, full length IL‑33 localizes to the nucleus in HUVECs and transfectants (2). The C‑terminal fragment, corresponding to mature IL‑33, binds and triggers signaling through mast cell IL‑1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5‑7). A ternary signaling complex is formed by the subsequent association of IL‑33 and ST2L with IL‑1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL‑33 in vitro induces IL‑5 and IL‑13 secretion (3). In vivo administration of mature IL‑33 promotes increased production of IL‑5, IL‑13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature human IL‑33 share
52‑58% aa sequence identity with mouse and rat IL‑33. Human IL‑33 shares less than 20% aa sequence identity with other IL‑1 family proteins.

References
  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Long Name
Interleukin 33
Entrez Gene IDs
90865 (Human); 77125 (Mouse)
Alternate Names
C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2

Citations for Recombinant Human IL-33 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

47 Citations: Showing 1 - 10
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  1. The Merkel Cell Polyomavirus T-Antigens and IL-33/ST2-IL1RAcP Axis: Possible Role in Merkel Cell Carcinoma
    Authors: K Rasheed, U Moens, B Policastro, JI Johnsen, V Koljonen, H Sihto, WO Lui, B Sveinbjørn
    International Journal of Molecular Sciences, 2022;23(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Temporal Quantitative Phosphoproteomics Profiling of Interleukin-33 Signaling Network Reveals Unique Modulators of Monocyte Activation
    Authors: DAB Rex, Y Subbannayy, PK Modi, A Palollathi, L Gopalakris, YP Bhandary, TSK Prasad, SM Pinto
    Cells, 2022;11(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. IL-33 and Superantigenic Activation of Human Lung Mast Cells Induce the Release of Angiogenic and Lymphangiogenic Factors
    Authors: L Cristinzia, R Poto, G Criscuolo, AL Ferrara, MR Galdiero, L Modestino, S Loffredo, A de Paulis, G Marone, G Spadaro, G Varricchi
    Cells, 2021;10(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Exogenous oxidative stress suppresses IL-33 -driven proliferation programming in group 2 innate lymphoid cells
    Authors: C Zheng, Z Lu, H Wu, L Cui, J Bi, X Wan
    International immunopharmacology, 2021;95(0):107541.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. p38 MAPK signalling regulates cytokine production in IL-33 stimulated Type 2 Innate Lymphoid cells
    Authors: T Petrova, J Pesic, K Pardali, M Gaestel, JSC Arthur
    Sci Rep, 2020;10(1):3479.
    Species: Human
    Sample Types: Cells
    Applications: Cell Culture
  6. Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis
    Authors: Y Imoto, T Takabayash, M Sakashita, Y Kato, K Yoshida, M Kidoguchi, K Koyama, N Adachi, Y Kimura, K Ogi, Y Ito, M Kanno, M Okamoto, N Narita, S Fujieda
    Biomolecules, 2020;10(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  7. TGF-&beta secreted by human umbilical cord blood-derived mesenchymal stem cells ameliorates atopic dermatitis by inhibiting secretion of TNF-&alpha and IgE
    Authors: HH Park, S Lee, Y Yu, SM Yoo, SY Baek, N Jung, KW Seo, KS Kang
    Stem Cells, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. IL-33/ST2 axis promotes glioblastoma cell invasion by accumulating tenascin-C
    Authors: JF Zhang, T Tao, K Wang, GX Zhang, Y Yan, HR Lin, Y Li, MW Guan, JJ Yu, XD Wang
    Sci Rep, 2019;9(1):20276.
    Species: Human
    Sample Types: cell culture
    Applications: Whole Cells
  9. Interleukin-33 delays recovery of mucosal inflammation via downregulation of homeostatic ABCG5/8 in the colon
    Authors: Y Mishima, H Sonoyama, S Ishihara, N Oshima, I Moriyama, K Kawashima, Y Kinoshita
    Lab. Invest., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. TNF-α and IL-17A induce the expression of lympho-epithelial Kazal-type inhibitor in epidermal keratinocytes
    Authors: S Sugihara, S Sugimoto, K Tachibana, M Kobashi, H Nomura, T Miyake, Y Hirai, O Yamasaki, S Morizane
    J. Dermatol. Sci., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  11. c-Kit-positive ILC2s exhibit an ILC3-like signature that may contribute to IL-17-mediated pathologies
    Authors: JH Bernink, Y Ohne, MBM Teunissen, J Wang, J Wu, L Krabbendam, C Guntermann, R Volckmann, J Koster, S van Tol, I Ramirez, Y Shrestha, MA de Rie, H Spits, X Romero Ros, AA Humbles
    Nat. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  12. The role of IL-36? and its regulation in eosinophilic inflammation in allergic rhinitis
    Authors: X Qin, M Liu, S Zhang, C Wang, T Zhang
    Cytokine, 2019;117(0):84-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Expression of c-Kit discriminates between two functionally distinct subsets of human type 2 innate lymphoid cells
    Authors: T Hochdörfer, C Winkler, K Pardali, J Mjösberg
    Eur. J. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. IL-33 Initiates Vascular Remodelling in Hypoxic Pulmonary Hypertension by up-Regulating HIF-1? and VEGF Expression in Vascular Endothelial Cells
    Authors: J Liu, W Wang, L Wang, S Chen, B Tian, K Huang, CJ Corrigan, S Ying, W Wang, C Wang
    EBioMedicine, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. IL-33 down-regulates CLDN1 expression through the ERK/STAT3 pathway in keratinocytes
    Authors: WI Ryu, H Lee, HC Bae, J Jeon, HJ Ryu, J Kim, JH Kim, JW Son, J Kim, Y Imai, K Yamanishi, SH Jeong, SW Son
    J. Dermatol. Sci., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  16. Soluble ST2 suppresses IL-5 production by human basophilic KU812 cells, induced by epithelial cell-derived IL-33
    Authors: K Matsumoto, H Kouzaki, H Kikuoka, T Kato, I Tojima, S Shimizu, T Shimizu
    Allergol Int, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Seasonal variation in circulating group 2 innate lymphoid cells in mugwort-allergic asthmatics during and outside pollen season
    Authors: Q Miao, Y Wang, YG Liu, YX Ren, H Guan, Z Li, W Xu, L Xiang
    Allergy Asthma Clin Immunol, 2018;14(0):6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. IL?33 restricts invasion and adhesion of trophoblast cell line JEG3 by downregulation of integrin ?4?1 and CD62L
    Authors: XH Wang, W Liu, DX Fan, WT Hu, MQ Li, XY Zhu, LP Jin
    Mol Med Rep, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. IL-33 blockade suppresses tumor growth of human lung cancer through direct and indirect pathways in a preclinical model
    Authors: K Wang, S Shan, Z Yang, X Gu, Y Wang, C Wang, T Ren
    Oncotarget, 2017;8(40):68571-68582.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. A functional DC cross talk promotes human ILC homeostasis in humanized mice
    Authors: S Lopez-Last, G Masse-Rans, O Fiquet, S Darche, N Serafini, Y Li, M Dusséaux, H Strick-Mar, JP Di Santo
    Blood Adv, 2017;1(10):601-614.
    Species: Human
    Sample Types: Whole Cells
  21. IL-33 promotes colon cancer cell stemness via JNK activation and macrophage recruitment
    Authors: M Fang, Y Li, K Huang, S Qi, J Zhang, W Zgodzinski, M Majewski, G Wallner, S Gozdz, P Macek, A Kowalik, M Pasiarski, E Grywalska, L Vatan, N Nagarsheth, W Li, L Zhao, I Kryczek, G Wang, Z Wang, W Zou, L Wang
    Cancer Res, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. SP and IL-33 together markedly enhance TNF synthesis and secretion from human mast cells mediated by the interaction of their receptors
    Authors: A Taracanova, M Alevizos, A Karagkouni, Z Weng, E Norwitz, P Conti, SE Leeman, TC Theoharide
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
  23. Interleukin-33 modulates inflammation in endometriosis
    Authors: JE Miller, SP Monsanto, SH Ahn, K Khalaj, AT Fazleabas, SL Young, BA Lessey, M Koti, C Tayade
    Sci Rep, 2017;7(1):17903.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. IL?33 enhances glioma cell migration and invasion by upregulation of MMP2 and MMP9 via the ST2-NF-?B pathway
    Authors: JF Zhang, P Wang, YJ Yan, Y Li, MW Guan, JJ Yu, XD Wang
    Oncol. Rep., 2017;38(4):2033-2042.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Effector and regulatory dendritic cells display distinct patterns of miRNA expression
    Authors: V Lombardi, S Luce, H Moussu, L Morizur, C Gueguen, C Neukirch, S Chollet-Ma, L Mascarell, M Aubier, V Baron-Bodo, P Moingeon
    Immun Inflamm Dis, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis
    Authors: D Díaz-Jimén, L Núñez, M De la Fuen, K Dubois-Cam, H Sepúlveda, M Montecino, A Torres-Riq, P García-Gon, J Chnaiderma, A Vossenkamp, TT MacDonald, D Simian, MJ González, JA Cidlowski, R Quera, MA Hermoso
    Sci Rep, 2017;7(1):10180.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  27. Functional IL-33 receptors are expressed in early progenitor stages of allergy-related granulocytes
    Immunology, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation
    Sci Rep, 2016;6(0):25171.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells
    Sci Rep, 2016;6(0):36795.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs
    Authors: SM Bal, JH Bernink, M Nagasawa, J Groot, MM Shikhagaie, K Golebski, CM van Drunen, R Lutter, RE Jonkers, P Hombrink, M Bruchard, J Villaudy, JM Munneke, W Fokkens, JS Erjefält, H Spits, XR Ros
    Nat Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Soluble IL-33 receptor sST2 inhibits colorectal cancer malignant growth by modifying the tumour microenvironment
    Nat Commun, 2016;7(0):13589.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production.
    Authors: Bu X, Zhang T, Wang C, Ren T, Wen Z
    J Transl Med, 2015;13(0):381.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins alpha-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators.
    Authors: Kempuraj D, Thangavel R, Yang E, Pattani S, Zaheer S, Santillan D, Santillan M, Zaheer A
    PLoS ONE, 2015;10(8):e0135776.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. IL-33 activates tumor stroma to promote intestinal polyposis.
    Authors: Maywald R, Doerner S, Pastorelli L, De Salvo C, Benton S, Dawson E, Lanza D, Berger N, Markowitz S, Lenz H, Nadeau J, Pizarro T, Heaney J
    Proc Natl Acad Sci U S A, 2015;112(19):E2487-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Interleukin-33/ST2 axis promotes epithelial cell transformation and breast tumorigenesis via upregulation of COT activity.
    Authors: Kim J, Lim S, Kim G, Yun H, Ahn S, Choi H
    Oncogene, 2015;34(38):4928-38.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  36. Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients.
    Authors: Teunissen M, Munneke J, Bernink J, Spuls P, Res P, Te Velde A, Cheuk S, Brouwer M, Menting S, Eidsmo L, Spits H, Hazenberg M, Mjosberg J
    J Invest Dermatol, 2014;134(9):2351-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. BCR-ABL-induced deregulation of the IL-33/ST2 pathway in CD34+ progenitors from chronic myeloid leukemia patients.
    Authors: Levescot A, Flamant S, Basbous S, Jacomet F, Feraud O, Anne Bourgeois E, Bonnet M, Giraud C, Roy L, Barra A, Chomel J, Turhan A, Guilhot F, Girard J, Gombert J, Herbelin A
    Cancer Res, 2014;74(10):2669-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. The alarmin concept applied to human renal transplantation: evidence for a differential implication of HMGB1 and IL-33.
    Authors: Thierry A, Giraud S, Robin A, Barra A, Bridoux F, Ameteau V, Hauet T, Girard J, Touchard G, Gombert J, Herbelin A
    PLoS ONE, 2014;9(2):e88742.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  39. Eosinophils regulate peripheral B cell numbers in both mice and humans.
    Authors: Wong, Tina W, Doyle, Alfred D, Lee, James J, Jelinek, Diane F
    J Immunol, 2014;192(8):3548-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Interleukin-33 induces urokinase in human endothelial cells--possible impact on angiogenesis.
    Authors: Stojkovic S, Kaun C, Heinz M, Krychtiuk K, Rauscher S, Lemberger C, de Martin R, Groger M, Petzelbauer P, Huk I, Huber K, Wojta J, Demyanets S
    J Thromb Haemost, 2014;12(6):948-57.
  41. Anti-inflammatory and anti-allergic activities of Pentaherb formula, Moutan Cortex (Danpi) and gallic acid.
    Authors: Liu K, Hu S, Chan B, Wat E, Lau C, Hon K, Fung K, Leung P, Hui P, Lam C, Wong C
    Molecules, 2013;18(3):2483-500.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  42. Muramyl dipeptide mediated activation of human bronchial epithelial cells interacting with basophils: a novel mechanism of airway inflammation.
    Authors: Qiu H, Wong C, Chu I, Hu S, Lam C
    Clin Exp Immunol, 2013;172(1):81-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. Daily exposure to dust alters innate immunity.
    Authors: Sahlander K, Larsson K, Palmberg L
    PLoS ONE, 2012;7(2):e31646.
    Species: Human
    Sample Types: Whole Blood
    Applications: Bioassay
  44. Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33.
    J. Exp. Med., 2012;209(8):1505-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  45. Serum neurotensin (NT) is increased in psoriasis and NT induces vascular endothelial growth factor release from human mast cells.
    Authors: Vasiadi M, Therianou A, Alysandratos KD, Katsarou-Katsari A, Petrakopoulou T, Theoharides A, Papadavid E, Stavrianeas N, Antoniou C, Kalogeromitros D, Theoharides TC
    Br. J. Dermatol., 2012;166(6):1349-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  46. A novel IL-1 family cytokine, IL-33, potently activates human eosinophils.
    Authors: Cherry WB, Yoon J, Bartemes KR, Iijima K, Kita H
    J. Allergy Clin. Immunol., 2008;121(6):1484-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  47. Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
    Authors: Hong YS, Moon S, Moon SJ, Joo YB, Joo Y, Jeon C, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK
    J Korean Med Sci, 0;26(9):1132-9.

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Reviews for Recombinant Human IL-33 Protein

Average Rating: 4.7 (Based on 6 Reviews)

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Recombinant Human IL-33 Protein
By Anonymous on 12/05/2021
Application: Cell Proliferation
Reason for Rating: Human SMCs were tested for proliferation in response to IL-33.

Recombinant Human IL-33 Protein
By Anonymous on 08/12/2021
Application: In vitro bioactivity in cell culture

Recombinant Human IL-33 Protein
By Anonymous on 06/09/2020
Application: ELISA standard

We used this protein in an in-house ELISA along with mAb (3625) and pAb (AF3625) to quantify IL-33 in human serum and plasma. This combination could not detect IL-33 in our samples but generated a good standard curve.


Recombinant Human IL-33 Protein
By Anonymous on 09/16/2019
Application: In vitro bioactivity in cell culture

By Anonymous on 03/29/2018
Application: In vitro bioactivity in cell culture

Recombinant Human IL-33 Protein
By Anonymous on 11/09/2017
Application: Immunoassay Standard

AF3625 was used as both the capture and the detection antibody for the sandwich ELISA for IL-33. The immunoassay standard was 3625-IL. Assay had sensitivity of ~1pg/ml. Many healthy human samples were not detectable.