Recombinant Human IL-33 Protein

  (29 citations)
(2 Reviews)
    
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Product Details
Citations (29)
FAQs
Reviews
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.06-0.24 ng/mL.
    Optimal dilutions should be determined by each laboratory for each application.
  • Source
    E. coli-derived human IL-33 protein
    Ser112-Thr270
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser112
  • Predicted Molecular Mass
    18 kDa
Product Datasheets

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Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3625-IL
 
3625-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human IL-33 (Catalog #
3625‑IL) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 0.06-0.24 ng/mL.
1 μg/lane of Recombinant Human IL-33 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.
Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1‑3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF‑HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL‑33 was identified based on sequence and structural homology with IL‑1 family cytokines (3). DVS 27, NF‑HEV, and IL‑33 share 100% amino acid sequence identity. IL‑33 is constitutively expressed in smooth muscle and airway epithelia. It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL‑1 alpha or IL‑1 beta stimulation (1, 3). Similar to IL‑1, IL‑33 can be cleaved in vitro by caspase‑1, generating an N‑terminal fragment that is slightly shorter than the C‑terminal fragment (3, 4). The N‑terminal portion of full length IL‑33 contains a predicted bipartite nuclear localization sequence and a homeodomain‑like helix‑turn‑helix DNA binding domain. By immunofluorescence, full length IL‑33 localizes to the nucleus in HUVECs and transfectants (2). The C‑terminal fragment, corresponding to mature IL‑33, binds and triggers signaling through mast cell IL‑1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5‑7). A ternary signaling complex is formed by the subsequent association of IL‑33 and ST2L with IL‑1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL‑33 in vitro induces IL‑5 and IL‑13 secretion (3). In vivo administration of mature IL‑33 promotes increased production of IL‑5, IL‑13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature human IL‑33 share
52‑58% aa sequence identity with mouse and rat IL‑33. Human IL‑33 shares less than 20% aa sequence identity with other IL‑1 family proteins.

  • References:
    1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
    2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
    3. Schmitz, J. et al. (2005) Immunity 23:479.
    4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
    5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
    6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. 95:6930.
    7. Dinarello, C.A. (2005) Immunity 23:461.
    8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  • Long Name:
    Interleukin 33
  • Entrez Gene IDs:
    90865 (Human); 77125 (Mouse)
  • Alternate Names:
    C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Seasonal variation in circulating group 2 innate lymphoid cells in mugwort-allergic asthmatics during and outside pollen season
    Authors: Q Miao, Y Wang, YG Liu, YX Ren, H Guan, Z Li, W Xu, L Xiang
    Allergy Asthma Clin Immunol, 2018;14(0):6.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Interleukin-33 modulates inflammation in endometriosis
    Authors: JE Miller, SP Monsanto, SH Ahn, K Khalaj, AT Fazleabas, SL Young, BA Lessey, M Koti, C Tayade
    Sci Rep, 2017;7(1):17903.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. IL-33 blockade suppresses tumor growth of human lung cancer through direct and indirect pathways in a preclinical model
    Authors: K Wang, S Shan, Z Yang, X Gu, Y Wang, C Wang, T Ren
    Oncotarget, 2017;8(40):68571-68582.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. IL?33 enhances glioma cell migration and invasion by upregulation of MMP2 and MMP9 via the ST2-NF-?B pathway
    Authors: JF Zhang, P Wang, YJ Yan, Y Li, MW Guan, JJ Yu, XD Wang
    Oncol. Rep., 2017;38(4):2033-2042.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis
    Authors: D Díaz-Jimén, L Núñez, M De la Fuen, K Dubois-Cam, H Sepúlveda, M Montecino, A Torres-Riq, P García-Gon, J Chnaiderma, A Vossenkamp, TT MacDonald, D Simian, MJ González, JA Cidlowski, R Quera, MA Hermoso
    Sci Rep, 2017;7(1):10180.
    Species: Human
    Sample Type: Whole Tissue
    Application: Bioassay
  6. SP and IL-33 together markedly enhance TNF synthesis and secretion from human mast cells mediated by the interaction of their receptors
    Authors: A Taracanova, M Alevizos, A Karagkouni, Z Weng, E Norwitz, P Conti, SE Leeman, TC Theoharide
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
  7. IL?33 restricts invasion and adhesion of trophoblast cell line JEG3 by downregulation of integrin ?4?1 and CD62L
    Authors: XH Wang, W Liu, DX Fan, WT Hu, MQ Li, XY Zhu, LP Jin
    Mol Med Rep, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. IL-33 promotes colon cancer cell stemness via JNK activation and macrophage recruitment
    Authors: M Fang, Y Li, K Huang, S Qi, J Zhang, W Zgodzinski, M Majewski, G Wallner, S Gozdz, P Macek, A Kowalik, M Pasiarski, E Grywalska, L Vatan, N Nagarsheth, W Li, L Zhao, I Kryczek, G Wang, Z Wang, W Zou, L Wang
    Cancer Res, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Functional IL-33 receptors are expressed in early progenitor stages of allergy-related granulocytes
    Immunology, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Soluble IL-33 receptor sST2 inhibits colorectal cancer malignant growth by modifying the tumour microenvironment
    Nat Commun, 2016;7(0):13589.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells
    Sci Rep, 2016;6(0):36795.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation
    Sci Rep, 2016;6(0):25171.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs
    Authors: SM Bal, JH Bernink, M Nagasawa, J Groot, MM Shikhagaie, K Golebski, CM van Drunen, R Lutter, RE Jonkers, P Hombrink, M Bruchard, J Villaudy, JM Munneke, W Fokkens, JS Erjefält, H Spits, XR Ros
    Nat Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. IL-33 activates tumor stroma to promote intestinal polyposis.
    Authors: Maywald R, Doerner S, Pastorelli L, De Salvo C, Benton S, Dawson E, Lanza D, Berger N, Markowitz S, Lenz H, Nadeau J, Pizarro T, Heaney J
    Proc Natl Acad Sci U S A, 2015;112(19):E2487-96.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins alpha-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators.
    Authors: Kempuraj D, Thangavel R, Yang E, Pattani S, Zaheer S, Santillan D, Santillan M, Zaheer A
    PLoS ONE, 2015;10(8):e0135776.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production.
    Authors: Bu X, Zhang T, Wang C, Ren T, Wen Z
    J Transl Med, 2015;13(0):381.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Interleukin-33/ST2 axis promotes epithelial cell transformation and breast tumorigenesis via upregulation of COT activity.
    Authors: Kim J, Lim S, Kim G, Yun H, Ahn S, Choi H
    Oncogene, 2015;34(38):4928-38.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  18. Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients.
    Authors: Teunissen M, Munneke J, Bernink J, Spuls P, Res P, Te Velde A, Cheuk S, Brouwer M, Menting S, Eidsmo L, Spits H, Hazenberg M, Mjosberg J
    J Invest Dermatol, 2014;134(9):2351-60.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. BCR-ABL-induced deregulation of the IL-33/ST2 pathway in CD34+ progenitors from chronic myeloid leukemia patients.
    Authors: Levescot A, Flamant S, Basbous S, Jacomet F, Feraud O, Anne Bourgeois E, Bonnet M, Giraud C, Roy L, Barra A, Chomel J, Turhan A, Guilhot F, Girard J, Gombert J, Herbelin A
    Cancer Res, 2014;74(10):2669-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  20. Interleukin-33 induces urokinase in human endothelial cells--possible impact on angiogenesis.
    Authors: Stojkovic S, Kaun C, Heinz M, Krychtiuk K, Rauscher S, Lemberger C, de Martin R, Groger M, Petzelbauer P, Huk I, Huber K, Wojta J, Demyanets S
    J Thromb Haemost, 2014;12(6):948-57.
  21. The alarmin concept applied to human renal transplantation: evidence for a differential implication of HMGB1 and IL-33.
    Authors: Thierry A, Giraud S, Robin A, Barra A, Bridoux F, Ameteau V, Hauet T, Girard J, Touchard G, Gombert J, Herbelin A
    PLoS ONE, 2014;9(2):e88742.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Eosinophils regulate peripheral B cell numbers in both mice and humans.
    Authors: Wong, Tina W, Doyle, Alfred D, Lee, James J, Jelinek, Diane F
    J Immunol, 2014;192(8):3548-58.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. Muramyl dipeptide mediated activation of human bronchial epithelial cells interacting with basophils: a novel mechanism of airway inflammation.
    Authors: Qiu H, Wong C, Chu I, Hu S, Lam C
    Clin Exp Immunol, 2013;172(1):81-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. Anti-inflammatory and anti-allergic activities of Pentaherb formula, Moutan Cortex (Danpi) and gallic acid.
    Authors: Liu K, Hu S, Chan B, Wat E, Lau C, Hon K, Fung K, Leung P, Hui P, Lam C, Wong C
    Molecules, 2013;18(3):2483-500.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  25. Daily exposure to dust alters innate immunity.
    Authors: Sahlander K, Larsson K, Palmberg L
    PLoS ONE, 2012;7(2):e31646.
    Species: Human
    Sample Type: Whole Blood
    Application: Bioassay
  26. Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33.
    J. Exp. Med., 2012;209(8):1505-17.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  27. Serum neurotensin (NT) is increased in psoriasis and NT induces vascular endothelial growth factor release from human mast cells.
    Authors: Vasiadi M, Therianou A, Alysandratos KD, Katsarou-Katsari A, Petrakopoulou T, Theoharides A, Papadavid E, Stavrianeas N, Antoniou C, Kalogeromitros D, Theoharides TC
    Br. J. Dermatol., 2012;166(6):1349-52.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  28. A novel IL-1 family cytokine, IL-33, potently activates human eosinophils.
    Authors: Cherry WB, Yoon J, Bartemes KR, Iijima K, Kita H
    J. Allergy Clin. Immunol., 2008;121(6):1484-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  29. Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
    Authors: Hong YS, Moon S, Moon SJ, Joo YB, Joo Y, Jeon C, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK
    J Korean Med Sci, 0;26(9):1132-9.
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Average Rating: 4.5 (Based on 2 reviews)

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We have 2 reviews tested in 2 applications: In vitro bioactivity in cell culture, Immunoassay Standard.

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In vitro bioactivity in cell culture Recombinant Human IL-33 Protein 3625-IL-010 Array 3625-IL
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 In vitro bioactivity in cell culture Anonymous 03/29/2018
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In vitro bioactivity in cell culture Recombinant Human IL-33 Protein 3625-IL-010
In vitro bioactivity in cell culture: Recombinant Human IL-33 Protein [3625-IL-010].

Summary

Application In vitro bioactivity in cell culture
Immunoassay Standard Recombinant Human IL-33 Protein 3625-IL Array 3625-IL
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 Immunoassay Standard Anonymous 11/09/2017
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Immunoassay Standard Recombinant Human IL-33 Protein 3625-IL
Immunoassay Standard: Recombinant Human IL-33 Protein [3625-IL].

Summary

Application Immunoassay Standard

Other Experimental Details

Other Experimental Details AF3625 was used as both the capture and the detection antibody for the sandwich ELISA for IL-33. The immunoassay standard was 3625-IL. Assay had sensitivity of ~1pg/ml. Many healthy human samples were not detectable.

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