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Recombinant Human IL-33 Protein

R&D Systems | Catalog # 3625-IL

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human IL-33 Protein (3625-IL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

O95760.1

Applications

Bioactivity
View all IL-33 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-33 protein
Ser112-Thr270

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser112

Predicted Molecular Mass

18-21 kDa

Activity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.
The ED50 for this effect is 0.06-0.24 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.

Reviewed Applications

Read 6 reviews rated 4.7 using 3625-IL in the following applications:

Scientific Data Images for Recombinant Human IL-33 Protein

Recombinant Human IL-33 Protein Bioactivity

Recombinant Human IL-33 Protein Bioactivity

Recombinant Human IL-33 (Catalog # 3625‑IL) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 0.06-0.24 ng/mL.
Recombinant Human IL-33 Protein SDS-PAGE

Recombinant Human IL-33 Protein SDS-PAGE

1 μg/lane of Recombinant Human IL-33 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 3625-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 3625-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
Reconstitution Reconstitute at 100-500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1‑3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF‑HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL‑33 was identified based on sequence and structural homology with IL‑1 family cytokines (3). DVS 27, NF‑HEV, and IL‑33 share 100% amino acid sequence identity. IL‑33 is constitutively expressed in smooth muscle and airway epithelia. It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL‑1 alpha or IL‑1 beta stimulation (1, 3). Similar to IL‑1, IL‑33 can be cleaved in vitro by caspase‑1, generating an N‑terminal fragment that is slightly shorter than the C‑terminal fragment (3, 4). The N‑terminal portion of full length IL‑33 contains a predicted bipartite nuclear localization sequence and a homeodomain‑like helix‑turn‑helix DNA binding domain. By immunofluorescence, full length IL‑33 localizes to the nucleus in HUVECs and transfectants (2). The C‑terminal fragment, corresponding to mature IL‑33, binds and triggers signaling through mast cell IL‑1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5‑7). A ternary signaling complex is formed by the subsequent association of IL‑33 and ST2L with IL‑1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL‑33 in vitro induces IL‑5 and IL‑13 secretion (3). In vivo administration of mature IL‑33 promotes increased production of IL‑5, IL‑13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature human IL‑33 share
52‑58% aa sequence identity with mouse and rat IL‑33. Human IL‑33 shares less than 20% aa sequence identity with other IL‑1 family proteins.

References

  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.

Long Name

Interleukin 33

Alternate Names

C9orf26, DVS27, IL33, NF-HEV

Entrez Gene IDs

90865 (Human); 77125 (Mouse)

Gene Symbol

IL33

UniProt

O95760.1

Additional IL-33 Products

Product Documents for Recombinant Human IL-33 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 3625-IL (with carrier)
Download SDS 3625-IL/CF (carrier free)

Product Specific Notices for Recombinant Human IL-33 Protein

For research use only

Citations for Recombinant Human IL-33 Protein

Customer Reviews for Recombinant Human IL-33 Protein (6)

4.7 out of 5
6 Customer Ratings
5 Stars
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Showing  1 - 5 of 6 reviews Showing All
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  • Recombinant Human IL-33 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 12/05/2021
    Recombinant Human IL-33 Protein 3625-IL
  • Recombinant Human IL-33 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 08/12/2021
    Recombinant Human IL-33 Protein 3625-IL
  • Recombinant Human IL-33 Protein
    Name: Anonymous
    Application: ELISA standard
    Verified Customer | Posted 06/09/2020
    We used this protein in an in-house ELISA along with mAb (3625) and pAb (AF3625) to quantify IL-33 in human serum and plasma. This combination could not detect IL-33 in our samples but generated a good standard curve.
    Recombinant Human IL-33 Protein 3625-IL
  • Recombinant Human IL-33 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 09/16/2019
    Recombinant Human IL-33 Protein 3625-IL
  • Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 03/29/2018
    Recombinant Human IL-33 Protein 3625-IL
  • Recombinant Human IL-33 Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 11/09/2017
    AF3625 was used as both the capture and the detection antibody for the sandwich ELISA for IL-33. The immunoassay standard was 3625-IL. Assay had sensitivity of ~1pg/ml. Many healthy human samples were not detectable.
    Recombinant Human IL-33 Protein 3625-IL

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